This invention belongs to the field of
molecular biology and relates to the application of ZmMIE2
protein in regulating maize kernel quality traits. The
amino acid sequence of the ZmMIE2
protein is shown in SEQ ID NO.2, and the
nucleotide sequence of the
gene encoding the ZmMIE2
protein is shown in SEQ ID NO.1. This invention discovers that the ZmMIE2 protein can regulate maize kernel quality traits, thereby reducing the concentration of certain nutrients in maize kernels. ZmMIE2
Gene overexpression leads to larger kernels, increased 100-kernel weight, and increased soluble
sugar content, which can be used to increase corn yield. ZmMIE2
Gene mutations can increase the
protein content of kernels, and this invention lays the theoretical foundation for creating new high-protein maize varieties. This discovery not only provides a scientific basis for understanding the development mechanism of kernels, but also provides theoretical guidance for the genetic improvement of maize.