Immobilized biological additive and its preparation method and uses

Abstract

Disclosed is a cigarette use biological addition agent and use thereof, wherein the biological enzyme comprises degraded proteinase, amylase, pectic enzyme, polysaccharide degraded enzyme, cellulose, surface active agent, enzyme activating agent, wall material, emulsifying agent, and cushioning liquid. The preparing process comprises the steps of agitating, immobilizing, and utilizing addition agent.

Description

technical field

[0001] The invention relates to a tobacco additive, more specifically, the invention relates to a tobacco additive prepared by biosolidification technology. Background technique

[0002] As we all know, new tobacco leaves have different degrees of quality defects, and are not suitable for direct use in the manufacture of cigarettes. Therefore, initial curing, re-curing and other treatment processes must be carried out. After rebaking, the internal quality of the tobacco leaves has been significantly improved compared with that of the first-cured tobacco leaves. However, the overall chemical composition of the tobacco leaves after rebaking is not coordinated. Or the biochemical process has not yet occurred, so the tobacco leaves after rebaking must be alcoholized.

[0003] Many components of tobacco leaves will change during the alcoholization process, such as protein, starch, nicotine, etc., undergo transformation and degradation rea...

Examples

Embodiment 1

[0023] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, ethyl stearate 0.5%, potassium tartrate 0.05 %, sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate added to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.

Embodiment 2

[0025] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, behenyl alcohol stearate 0.5%, tartaric acid Potassium 0.05%, sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate added to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.

Embodiment 3

[0027] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, triglyceride 0.5%, potassium tartrate 0.05%, Sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate add up to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.

technical field

[0001] The invention relates to a tobacco additive, more specifically, the invention relates to a tobacco additive prepared by biosolidification technology. Background technique

[0002] As we all know, new tobacco leaves have different degrees of quality defects, and are not suitable for direct use in the manufacture of cigarettes. Therefore, initial curing, re-curing and other treatment processes must be carried out. After rebaking, the internal quality of the tobacco leaves has been significantly improved compared with that of the first-cured tobacco leaves. However, the overall chemical composition of the tobacco leaves after rebaking is not coordinated. Or the biochemical process has not yet occurred, so the tobacco leaves after rebaking must be alcoholized.

[0003] Many components of tobacco leaves will change during the alcoholization process, such as protein, starch, nicotine, etc., undergo transformation and degradation rea...

Examples

Embodiment 1

[0023] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, ethyl stearate 0.5%, potassium tartrate 0.05 %, sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate added to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.

Embodiment 2

[0025] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, behenyl alcohol stearate 0.5%, tartaric acid Potassium 0.05%, sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate added to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.

Embodiment 3

[0027] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, triglyceride 0.5%, potassium tartrate 0.05%, Sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate add up to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.