High plant cell wall degradation activity rumen fungi, and applications thereof in feed ensiling
A technology of plant cells and fungi, applied in the preservation method of animal feed raw materials, animal feed, application, etc., can solve problems that have not been widely used in production, and achieve the effect of improving fermentation quality and degradation rate
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[0055] In addition, the embodiment of the present invention discloses a method for preparing a combined enzyme for plant cell wall degradation, including the steps of fermenting and culturing Piromyces sp. CN6 and purifying the enzyme in the fermentation broth.
[0056] The invention also discloses a method for preparing crop straw silage, which includes the step of adding Piromyces sp.CN6 bacterium or culture solution in the silage project.
[0057]In a preferred embodiment, add Piromyces sp.CN6 thalline 10 5 TFU / g or the number of fungi in the culture solution >10 6 TFU ml -1 .
[0058] In a preferred embodiment, the method for preparing silage further includes the step of fermenting and culturing Piromyces sp. CN6 before silage.
[0059] In a preferred embodiment, the crop stalks are corn stalks.
Embodiment 1
[0061] Example 1 Isolation and identification of rumen fungi with high plant cell wall degrading enzyme activity and analysis of enzymatic characteristics
[0062] 1.1 Materials and methods
[0063] The source of the strain: the rumen content was taken from the animal husbandry teaching base of Northwest Agriculture and Forestry University, three Xinong Saanen dairy goats equipped with permanent rumen fistula and in good body condition, the experimental animals were fed once at 08:30 and 17:30 respectively , the ratio of dietary concentrate to crude food was 3:7, free drinking water, dietary composition and nutritional level are shown in Table 1. Collection of rumen contents: 2 hours after morning feeding of dairy goats, the rumen contents are collected from the fistula through a negative pressure device, and filled with sterilized and filled with CO 2 Put the anaerobic bottle into a thermos bottle at 39°C and quickly bring it back to the laboratory for isolation and culture ...
Embodiment 2
[0107] Example 2 Effects of Piromyces sp.CN6 on the Fermentation Quality, Nutrients and In Vitro Digestibility of Whole Plant Corn Silage
[0108] 2.1 Materials and methods
[0109] Silage raw material: Whole-plant corn (Zhongyuan Dan 32) cultivated in the Animal Husbandry Teaching Base of Northwest A&F University, mowed at the milky stage, with a water content of about 64%, crushed to 1-3cm. The composition of the whole plant corn is shown in Table 4.
[0110] Table 4 Nutrient composition of whole corn
[0111]
[0112] Composite enzyme preparation: special additive for silage produced by Japan Snow Brand Seed Company, solid dosage form, containing cellulase and xylanase, the activities are 90FPU / g and 6000IU / g respectively.
[0113] Fungal inoculant: strain Piromyces sp.CN6 is a rumen anaerobic fungus with high plant cell wall degrading enzyme activity isolated from Xinong Shaneng dairy goats in the laboratory. 6 TFUml -1 .
[0114] The experiment adopted a single fa...
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