8results about How to "Increase lactic acid content" patented technology

The invention discloses capsaicin-tolerant lactobacillus plantarum and application thereof in pickled pepper fermentation, and belongs to the technical field of microorganisms. The lactobacillus plantarum MYL-6 provided by the invention is preserved in the China Center for Type Culture Collection on August 10, 2023, and the preservation number of the lactobacillus plantarum MYL-6 is CCTCC (China Center for Type Culture Collection) M 20231407. According to the invention, a fusion strain MYL-6 is constructed through inactivation of parents and protoplast fusion. Experimental results prove that the prepared lactobacillus plantarum strain MYL-6 not only has the capacity of producing lactic acid at high yield, but also has the characteristic of capsaicin tolerance, after the lactobacillus plantarum provided by the invention is used for fermenting pickled peppers, the lactic acid content is increased, the acid fragrance is improved, the fermentation period of the pickled peppers is shortened, the quality of the pickled peppers can be improved, and the production cost is reduced. The method has huge application potential in the pickled pepper manufacturing industry.

The invention relates to the technical field of biology, in particular to a method for solid state fermentation of bagasse by using white rot fungi, which comprises the following steps: (1) activation and propagation of strains: pouring the white rot fungi into a PDA culture medium for activation and propagation; (2) raw material preparation: air-drying fresh bagasse, adding water to adjust the water content of the bagasse to 60-70%, and adjusting the pH to 4-6 with glacial acetic acid; and (3) preparing the bagasse feed, namely mixing the white rot fungi subjected to expanding culture according to 1-5% of the weight of an air-dried substance with bagasse of which the initial pH is adjusted to be 4-6, adding 1-5% of soluble sugar, and fermenting for 10-45 days to obtain the fermented bagasse feed. According to the white rot fungus fermented bagasse prepared by the method disclosed by the invention, the hemicellulose content of the bagasse can be reduced to 26.62%-22.45%, the lignin content can be reduced to 10.67%-8.66%, the crude protein content of the bagasse can be increased to 1.61%-2.12%, the lactic acid content can be increased to 26.57 mg / L-181.69 mg / L, and high-quality feed application of the bagasse can be realized.

The application discloses a preparation method and application of low-protein probiotic fermented feed, relates to the technical field of fermented feed and low-protein daily ration, and comprises the following steps: S1, preparing a low-protein fermented base material, wherein the low-protein fermented base material comprises, in mass parts, 45-70 parts of cereal raw materials, 6-22 parts of plant protein by-products, 8-28 parts of bran fiber raw materials, 1-6 parts of fermentable sugar sources, 0.2-2.0 parts of mineral buffer components and 0.15-2.50 parts of amino acid balancing components, and the crude protein content of the low-protein fermented base material is controlled to be 10.0-16.5 wt% on a dry basis; and S2, performing steam conditioning or wet heat curing treatment on the low-protein fermented base material after being crushed and mixed to obtain a pretreated base material. Through the combination of the low-protein base material design and the amino acid balancing components, the crude protein content of the product is stably controlled to be 10.0-16.5 wt%, and meanwhile, the limiting amino acids such as lysine, methionine, threonine, tryptophan, valine and isoleucine are supplemented, so that the problem of amino acid mode imbalance caused by the reduction of protein raw materials is avoided.

The present application provides a bacillus licheniformis strain HSCY3018, which is preserved in China Center for Type Culture Collection, and the preservation number is CCTCCM20221831, and the preservation date is November 28, 2022. The present application also provides the application of the HSCY3018 strain and a microbial inoculum thereof in food vinegar brewing. The HSCY3018 has high amylase and protease activities in food vinegar brewing, and can significantly improve the raw material utilization rate of food vinegar brewing. Compared with a control group, the starch utilization rate is increased by 58.50%, the lactic acid content in organic acid is increased by 72.95%, the total amount of amino acid is increased by 66.18%, the reducing sugar content is increased by 43.96%, and the total ester content is increased by 275.74%.

The application discloses a kind of low-protein probiotic fermented feed and preparation method thereof, it is related to animal feed and biological fermentation feed technical field, it is prepared from the following raw materials: energy class raw material 45-70 parts, plant protein raw material 8-25 parts, fiber carrier 8-25 parts, fermentable sugar source 1-8 parts, amino acid balance component 0.40-3.00 parts, mineral buffer component 0.20-2.00 parts, complex enzyme preparation 0.05-0.60 parts, probiotic fermentation inoculum 0.05-1.50 parts and water 18-45 parts;Low-protein formula design is combined with probiotic fermentation process, by controlling the crude protein mass content of final product is 12.0-17.5%, and cooperate with lysine, methionine, threonine, tryptophan and other limiting amino acid supplement, make product in reducing protein raw material dosage, still maintain key amino acid supply.

The invention relates to the field of biological fermentation feed, and particularly discloses a compound enzyme preparation for fermenting rice bran and feed rich in rice bran, application and preparation of the fermented feed. The complex enzyme preparation is composed of cellulase, beta-mannase and beta-glucosidase, rice bran and biomass materials rich in the rice bran are fermented through bacteria and enzymes in a synergistic mode, antinutritional factors of the rice bran or biomass rich in the rice bran are reduced, the nutritional value of the rice bran or fermented feed rich in the biomass rich in the rice bran is increased, and resource waste is reduced. The fermented rice bran feed and the preparation method thereof have a wide application prospect.

The present application belongs to the field of microbial application and feed processing technology, and discloses a bacillus subtilis, named bacillus subtilis YT1, which is preserved in China Center for Type Culture Collection, located at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, with a preservation number of CCTCC NO: M 20242767 and a preservation date of December 10, 2024. The present application also discloses a microbial preparation and the application of the bacillus subtilis or the microbial preparation in the processing of silage. The present application first simultaneously possesses high efficient lignocellulose degradation ability, excellent fermentation promotion characteristics, significant antioxidant activity and good probiotic characteristics in a strain of bacillus subtilis. This characteristic enables a single bacterial agent to achieve multiple goals such as fiber degradation, fermentation promotion, oxidation protection, which traditionally requires multiple compounded bacterial agents, and fundamentally overcomes the problems of poor compatibility between strains, unstable synergistic effect and high application cost of existing compounded additives.

This invention provides a method for preparing a high-protein bamboo shoot shell feed additive, relating to the field of animal feed preparation technology. The high-protein bamboo shoot shell feed additive is prepared by drying and pulverizing bamboo shoot shells, fermenting them with a liquefied Serratia marcescens bacterial solution containing chrysanthemum powder, followed by sterilization and enzymatic hydrolysis, and finally adding lactic acid bacteria for silage. This invention overcomes the shortcomings of existing technologies, effectively increasing the protein content of bamboo shoot shells while allowing them to be used as a feed additive, enhancing animal immunity, and comprehensively improving the economic benefits of its use.