Polysialic acid fermentation culture medium and method for producing polysialic acid through escherichia coli fermentation

Abstract

The invention provides a polysialic acid fermentation culture medium and a method for producing polysialic acid through escherichia coli fermentation. The polysialic acid fermentation culture medium is a total synthesis fermentation culture medium; compound amino acid is creatively used as a nitrogen source of the fermentation culture medium; problems like escherichia coli apoptosis in advance inthe fermentation process due to the facts that traditional natural organic matters like corn steep liquor dry powder is high in lactic acid content, and metabolic pressure is applied to the escherichia coli in the fermentation process, especially in the large-scale fermentation process are avoided; in addition, the compound amino acid culture medium can improve a material conversion rate and prevent metabolic abnormality of the escherichia coli; and finally, the yield of the polysialic acid can be increased after the production scale is enlarged, and good process consistency is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, relates to a polysialic acid fermentation medium and an application thereof, in particular to a polysialic acid fermentation medium and a method for producing polysialic acid by Escherichia coli fermentation. Background technique [0002] Sialic acid is a derivative of 9-carbon sugar, the most common of which is N-acetylneuraminic acid (Neu5Ac). Neu5Ac is involved in multiple physiological processes such as cell recognition, signal transduction, tumorigenesis, and fertilization. It can also regulate the anti-inflammatory activity of IgG, enhance infant immunity, affect the integrity, permeability, and activity of nerve cells, and promote infant brain development. . [0003] Polysialic acid PSA is a linear homopolymer of N-acetylneuraminic acid connected by α-2,8 and (or) α-2,9 glycosidic bonds, and the final product sialic acid can be obtained after acid hydrolysis. At present, ...

AI Technical Summary

Problems solved by technology

At present, most of the media used for fermentation and production of PSA are semi-synthetic media, the fermentation yield is limited, the synthesis efficiency and material conversion rate are low, and the fluctuation between batches is large, especially after the scale is scaled up. The fluctuation is more obvious, which limits the application of N-acetylneuraminic acid

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