Liquid bacterial liquid for fermenting liquid piglet feed, and preparation method and application of piglet liquid fermented feed

A liquid feed, liquid fermentation technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc., can solve the problems of early weaning losing expected value, etc., to enhance the immune function and growth rate of piglets. Speed ​​up and kill pathogenic microorganisms

Inactive Publication Date: 2020-07-31
博益德(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] As the weaning age of piglets continues to advance, they are facing the challenge of "earl

Method used

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  • Liquid bacterial liquid for fermenting liquid piglet feed, and preparation method and application of piglet liquid fermented feed
  • Liquid bacterial liquid for fermenting liquid piglet feed, and preparation method and application of piglet liquid fermented feed
  • Liquid bacterial liquid for fermenting liquid piglet feed, and preparation method and application of piglet liquid fermented feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Screening of fermentation strains

[0031] The present invention screens target strains from 9 strains in the strain bank owned by Boyide (Beijing) Biotechnology Co., Ltd. The strains can be divided into 4 strains of Bacillus according to their categories, namely Bacillus licheniformis BFC190102 (CGMCC NO.18911) marked as B1, Bacillus subtilis BFC190103 (CGMCC NO.18912) marked as B2, Bacillus subtilis BFC1601 marked as B3, Bacillus amyloliquefaciens BFC190306 is marked as B4; 5 strains of lactic acid bacteria are marked as R1 for Lactobacillus plantarum BFC190204 (CGMCC NO.18915), R2 for Enterococcus faecalis BFC190205 (CGMCC NO.18916), R3 for Bacillus coagulans BFC190307, Lactobacillus soybean meal BFC1607 is marked as R4, and Lactobacillus fermentum BFC1609 is marked as R5.

[0032] 1) Screening of Bacillus strains

[0033] Spot the activated spores on starch medium (0.5g of beef extract, 1g of peptone, 0.5g of sodium chloride, 0.2g of soluble starch, 100mL of w...

Embodiment 2

[0084] A method for preparing liquid fermented feed for piglets, comprising the following methods,

[0085] (1) The preparation of the liquid bacterium liquid that is used for fermenting piglet liquid feed, it is to be combined by spore liquid and lactic acid bacterium liquid; It is a mixed bacterial solution of Lactobacillus plantarum and Enterococcus faecalis. The specific operation is to culture lactic acid bacteria: Lactobacillus plantarum and Enterococcus faecalis with MRS respectively based on a 40°C incubator for 36 hours, and Bacillus: Bacillus licheniformis and Bacillus subtilis respectively with LB culture based on a 37°C shaker at 180r / min, Cultivate for 18 hours, mix the obtained Lactobacillus plantarum liquid and the Enterococcus faecalis liquid in a volume ratio of 1:2 (V / V) to obtain a lactic acid bacteria liquid, and mix the Bacillus licheniformis liquid and the Bacillus subtilis liquid in a volume ratio of 1 : 1 (V / V) mixing to obtain the spore liquid, the sp...

Embodiment 3

[0092] Piglet liquid fermented feed made with the method of embodiment 2, feeding 7 days old-15 days old piglets, altogether 24, divide two treatment groups, each treatment group repeats three times, and the control group feeds full-price powder ( The full-price powder was provided by Tianjin Mufeng Feed Co., Ltd.), and the liquid fermented feed of piglets in the test group replaced 50% of the full-price powder.

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Abstract

The invention provides a liquid bacterial liquid for fermenting a piglet liquid feed, and a preparation method and application of the piglet liquid fermented feed. The liquid bacterial liquid for fermenting the piglet liquid feed comprises a bacillus liquid and a lactic acid bacteria liquid; the bacillus liquid is a mixed bacteria liquid of bacillus licheniformis and bacillus subtilis; and the lactic acid bacteria liquid is a mixed bacteria liquid of lactobacillus plantarum and enterococcus faecalis. The piglet liquid fermented feed is a feed fermented by adopting the liquid bacterial liquid for fermenting the piglet liquid feed. The liquid bacterial liquid provided by the invention can improve the utilization rate of the feed, improve the flavor of the feed and improve the palatability, and after the feed is fermented, the contents of reducing sugar and lactic acid are obviously increased, the probiotic number is obviously increased, and the feed has acid fragrance. The piglet liquidfermented feed contains a large amount of probiotics, and is mainly used for enhancing the immune function of the piglets, killing pathogenic microorganisms and preventing diseases such as diarrhea ofthe piglets by fermenting to generate lactic acid and secrete some antibacterial substances.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to the technical field of microbial fermented feed, in particular to a preparation method and application of a liquid bacterial solution for fermenting liquid piglet feed and piglet liquid fermented feed. Background technique [0002] As the weaning age of piglets continues to advance, they are facing the challenge of "early weaning syndrome". If it is not effectively solved, early weaning will lose its expected value. For a long time, people have been used to adding antibiotics to enhance the immunity of piglets, so as to control various diseases such as diarrhea. After extensive and long-term use of antibiotics, the negative effects of antibiotics are increasing. Today, when food safety is of great concern, people all over the world are banning antibiotics one after another. How to improve the utilization rate of feed, improve the flavor of feed, improve palatability, preven...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K50/30A23K50/60C12N1/20C12R1/25C12R1/125C12R1/10C12R1/01
CPCA23K10/12A23K50/30A23K50/60C12N1/20A23V2400/169
Inventor 张孟阳刘世杰陈达于继英任婉丽张妮妮
Owner 博益德(北京)生物科技有限公司
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