91 results about "Enterococcus fecalis" patented technology

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention belongs to the technical field of biology, and provides enterococcus faecalis, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.29087. The enterococcus faecalis is characterized in that the enterococcus faecalis can secrete bacteriocin for inhibiting vibrio parahaemolyticus, staphylococcus aureus and escherichia coli. The invention also provides the enterococcus faecalis and application thereof in an aquatic product fermented feed and a laying hen premix, and the enterococcus faecalis can effectively improve the utilization rate of the feed and the growth and production performance of animals.

The invention belongs to the technical field of microorganisms, and discloses a Lactobacillus paracasei B20 for inhibiting melanin from a plateau area and application of the Lactobacillus paracasei B20, the Lactobacillus paracasei B20 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32434, and the preservation date is October 31, 2024. Metabolite of the lactobacillus paracasei B20 comprises phenylalanine, p-hydroxycinnamic acid and the like, has strong tyrosinase inhibition effect and anti-ultraviolet ability, and can significantly reduce generation of melanin in skin. In addition, the lactobacillus paracasei B20 also has relatively strong oxidation resistance, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body. In addition, the lactobacillus paracasei B20 has a relatively obvious growth inhibition effect on skin conditioned pathogens such as staphylococcus aureus and enterococcus faecalis.

The invention relates to the technical field of microorganisms, in particular to enterococcus faecalis EF-1 capable of reducing blood lipid, a microbial preparation and application of the enterococcus faecalis EF-1. The enterococcus faecalis EF-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 27, 2024, and the preservation number is CGMCC No.32097. The enterococcus faecalis EF-1 is named as enterococcus faecalis EF-1. The enterococcus faecalis EF-1 has the functions of: (1) relieving the weight increase speed of animals, reducing body weight gain, and preventing, relieving or treating obesity; (2) the contents of triglyceride, cholesterol and low-density lipoprotein cholesterol in liver and serum are reduced; (3) the liver function is protected, and the contents of ALT and AST in serum and liver are reduced; (4) liver lipid deposition and lesion are relieved; (5) relieving adipose tissue hypertrophy; (6) preventing, relieving or treating metabolic syndrome; (7) reducing blood sugar, improving insulin resistance and relieving diabetes mellitus; 8, increasing the content of short-chain fatty acid; and (9) regulating intestinal flora.

The invention discloses a production process of hermetia illucens-soybean meal fermented feed and application of the hermetia illucens-soybean meal fermented feed in preparation of rana nigromaculata feed, and belongs to the field of insect resource utilization. The preparation method comprises the following steps: preparing a bacterial liquid from a composite fermentation bacterial agent, compounding adult black soldier fly larvae into pulp, compounding the pulp with soybean meal to obtain a mixture, adding the bacterial liquid into the mixture, and carrying out microbial fermentation to prepare the black soldier fly-soybean meal fermented feed. Wherein the compound fermentation bacterial agent comprises bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, saccharomyces cerevisiae and enterococcus faecalis. According to the black soldier fly-soybean meal fermented feed produced by the invention, the processing and storage cost of black soldier fly larvae is reduced to the greatest extent, and when the black soldier fly-soybean meal fermented feed is applied to a compound feed of rana nigromaculata, the use amount of fish meal in an aquatic feed can be reduced, and the black soldier fly-soybean meal fermented feed can effectively promote growth, improve intestinal health, improve internal immunity and disease resistance and effectively prevent and control diseases; and fishery medicine application is reduced.

A strain of Enterococcus faecalis, a composite bacterial agent, and application thereof, which relates to the field of microbiology. The Enterococcus faecalis strain was deposited on Oct. 13, 2022, at the Guangdong Microbial Culture Collection Center (GDMCC) with the deposit number GDMCC No. 62869. Both the Enterococcus faecalis strain and the composite bacterial agent can effectively treat intestinal disorders and inflammatory bowel disease in felines and can be used in the preparation of related pharmaceuticals.

The invention relates to a composite microbial inoculant and an evaluation method for the antibacterial effect thereof. The method optimizes the in-vitro culture mode of the microbial agent compounded by aerobic bacteria and anaerobic bacteria by simulating the intestinal environment of probiotics in an animal body, and adopts a facultative anaerobic culture method for the microbial compound agent, namely, the outer layer of a sealing film of a triangular flask is wrapped with four layers of preservative films, and the neck of the triangular flask is tightened by a rubber band. The facultative anaerobic environment of aerobic bacillus and facultative anaerobic enterococcus faecalis is considered in the microbial composite inoculant, in the subsequent oxford cup bacteriostasis evaluation experiment, the experiment result with the accurate bacteriostasis effect and good parallelism can be obtained, and the action effect of bacillus and enterococcus faecalis composite bacteria can be efficiently and scientifically evaluated.

The invention relates to the technical field of biological breeding, and discloses a composite microecological preparation for adjusting lamb intestinal flora and relieving weaning stress, the composite microecological preparation comprises, by weight, 10-40 parts of enterococcus faecalis powder, 10-40 parts of bacillus subtilis powder and 10-40 parts of saccharomycetes powder, the enterococcus faecalis powder is prepared through a gradient acid-resistant pre-domestication system, and the bacillus subtilis powder is prepared through a gradient acid-resistant pre-domestication system. The bacillus subtilis powder is prepared through an oxygen-controlled induced spore strengthening process, the saccharomycetes powder is prepared through a beta-glucan strengthening reactor, and the three kinds of bacterium powder are mixed under the sterile condition to obtain the bacillus subtilis powder, the saccharomycetes powder and the beta-glucan strengthening reactor. The tolerance, activity and stability of the strain are improved, the controllable and repeatable preparation process of the compound microecological preparation is ensured, and a reliable technical basis is provided for regulating intestinal flora of weaned lambs and relieving weaning stress.

An object of the present invention is to provide a new lytic agent capable of lysing Enterococcus bacteria. Provided is a lytic agent for Enterococcus bacteria, consisting of a lytic enzyme or an active fragment thereof, the lytic enzyme comprising: (a) the amino acid sequence shown in SEQ ID NO: 1, (b) an amino acid sequence in which one or multiple amino acids are added, deleted, and / or substituted in the amino acid sequence shown in SEQ ID NO: 1, or (c) an amino acid sequence having a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 1.

The present application provides a kind of complex probiotic paste, which combines Enterococcus faecalis, Saccharomyces cerevisiae and vitamin B 12 Producing lactic acid bacteria of canine source, adds prebiotics, flavoring substances and auxiliary substances such as paste matrix preparation.In the paste, the viable count of Enterococcus faecalis, Saccharomyces cerevisiae and lactic acid bacteria is 1-2:1-2:1-8;Paste matrix includes fructooligosaccharide 0.5-3%, psyllium husk powder 0.1-2%, goat milk powder 2-8%, chicken liver powder 1-5%, potassium sorbate 0.5-2%, xanthan gum 1-4%, and the balance is water.The above-mentioned complex probiotic paste can be stably stored for 56 days at 4 DEG C and normal temperature dry place, has good storage stability, good safety, and can adjust intestinal flora and increase the content of intestinal lactic acid bacteria.The above-mentioned complex probiotic paste has good palatability and oral tolerance for pets such as dogs and cats, and has good therapeutic effect on DSS-induced chronic enteritis diarrhea and vitamin B 12 Deficiency.

The utility model discloses mixing equipment for preparing enterococcus faecalis, which comprises a tank body, the top of a tank cover is rotatably connected with a rotating plate, one side of the top of the rotating plate is connected with a driving mechanism which is convenient for the rotating plate to rotate, the top of the rotating plate is provided with a feeding mechanism which is convenient for adding auxiliary materials, and the inner side of the tank body is provided with a stirring mechanism which is convenient for uniformly mixing the auxiliary materials. According to the utility model, auxiliary materials can be conveniently added into the tank body in a closed environment through the arranged feeding mechanism, the arranged driving mechanism can drive the feeding mechanism to rotate, so that the auxiliary materials can be more uniformly added, and the arranged stirring mechanism can efficiently stir and uniformly mix the auxiliary materials, so that the auxiliary materials can be more uniformly added through the structure. According to the enterococcus faecalis culture equipment, the situation that many infectious microbes enter the culture equipment through air exchange to damage the culture environment can be effectively avoided, the survival rate of the enterococcus faecalis is increased, meanwhile, auxiliary materials can be added more uniformly, and the overall growth condition of the enterococcus faecalis is guaranteed.

The present application relates to the technical field of microbial feed additive, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the following steps: taking Lactobacillus plantarum and / or Enterococcus faecalis as a bacterial strain to activate and expand culture, taking agricultural and sideline product waste as a substrate to obtain mature fermentation liquor through deep liquid fermentation; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide to obtain a semi-dry granule core through granulation and low-temperature pre-drying; and coating the semi-dry granule core with a κ-carrageenan / konjac glucomannan / plant sterol ester composite gel, and drying and packaging to obtain a finished product.The double essential oil, chitosan and theabrownin jointly form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; the porous silicon dioxide actively maintains low water activity of the granule to prevent the lactic acid bacteria from recovering in advance; and the product has high viable count, strong heat and acid resistance, and excellent storage stability.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The invention provides an enterococcus faecalis FS and application thereof. The enterococcus faecalis FS is preserved in the Guangdong Microbial Culture Collection Center on May 12, 2025, and the preservation number of the enterococcus faecalis FS is GDMCC (China General Microbiological Culture Collection Center) 66318. The novel enterococcus faecalis FS is separated from the intestinal tract of healthy adult largemouth bass, has good acid and alkali resistance, cholate resistance, acid production and antibacterial capacity, is good in biological safety and has no toxic or side effect on fishes. When the enterococcus faecalis FS provided by the invention is added into juvenile fish functional feed as a core component, not only can the growth performance and disease resistance of largemouth bass be improved, but also the use of antibiotics can be reduced, the environmental pollution can be reduced, the aquaculture industry can be promoted to develop towards a high-efficiency, environment-friendly and sustainable direction, and the enterococcus faecalis FS has important application value and market prospect.

The invention relates to the technical field of textile detection, in particular to a method for detecting enterococcus faecalis in textiles, which comprises the following steps: S1, sample preparation: selecting a weighing method, a multi-point sampling elution method or a cotton swab smearing method according to the types of the textiles to prepare a sample homogenate; step S2, dilution and inoculation: performing 10-time series dilution on the sample homogenate, selecting a sample with 2-3 continuous dilution degrees, inoculating the sample to a bile aesculin sodium azide agar culture medium, and culturing at 36 + / -1 DEG C for 24-48 hours. According to the method, the detection technology of enterococcus faecalis in the textile is innovatively adopted, and the pollution condition of enterococcus faecalis in the textile is effectively monitored; the method has the following remarkable advantages that operation is easy and convenient, cost is low, complex instruments and equipment are not needed, the method is suitable for conventional detection of a primary laboratory, and the technology has outstanding originality and practicability in the aspect of improving the biological safety prevention and control capacity of the textile.

The invention discloses enterococcus faecalis for degrading uric acid in an anaerobic environment and application of the enterococcus faecalis, and belongs to the technical field of microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center of Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing on February 19, 2025, and the preservation number is CGMCC No.33565. The enterococcus faecalis with the uric acid reducing effect is obtained by separating and culturing healthy human intestinal tracts, and the enterococcus faecalis can directly metabolize uric acid in an anaerobic environment and has an excellent uric acid degrading effect.

The present invention discloses a novel antimicrobial peptide and its application. The present invention optimizes the structure of Epi-1, cuts off the non-α regions at both ends, and obtains a target antimicrobial peptide with a 16-amino acid sequence by replacing individual amino acids, thereby obtaining a novel antimicrobial polypeptide F-3 with a shorter sequence, higher antimicrobial activity, and better biocompatibility. A nucleic acid molecule encoding the antimicrobial peptide and an expression vector or recombinant cell containing the nucleic acid molecule are also provided. In vitro verification found that compared with the parent peptide, the derived peptide F-3 has higher antimicrobial activity or a higher bactericidal rate, and the minimum inhibitory concentration of F-3 is less than that of the parent peptide Epi-1; F-3 can also effectively inhibit or eliminate the formation of Enterococcus faecalis biofilm, and has low toxicity and good biocompatibility, and can be used to prepare drugs for preventing and treating diseases caused by Enterococcus faecalis infection; and the shorter amino acid sequence reduces the synthesis cost, and has good application prospects.

The application belongs to the technical field of biotechnology, and provides application of an engineered Enterococcus faecalis capable of expressing human phenylalanine hydroxylase in a medicine for treating phenylketonuria, a plasmid with a human-derived phenylalanine hydroxylase gene after codon optimization of the Enterococcus faecalis is introduced into the Enterococcus faecalis, so that the human phenylalanine hydroxylase is expressed in the intestinal tract. Experimental results show that the human phenylalanine hydroxylase is successfully expressed in the Enterococcus faecalis through genetic engineering technology; the engineered Enterococcus faecalis can significantly metabolize phenylalanine in an in-vitro culture medium system; meanwhile, the engineered Enterococcus faecalis can significantly treat phenylketonuria, and can reduce plasma phenylalanine of a phenylketonuria suckling mouse by 46.8% after 4 weeks of treatment. It is indicated that the engineered Enterococcus faecalis has an outstanding therapeutic effect in preventing and / or treating phenylketonuria.

The invention discloses a broiler feed additive containing chlorella and application of the broiler feed additive. The additive contains chlorella; the feed also contains one or more of lactobacillus johnsonii, enterococcus faecalis, saccharomyces cerevisiae and bacillus amyloliquefaciens. By adding the additive disclosed by the invention into daily ration, the intestinal tissue form can be improved by regulating antioxidant and immune functions, so that the growth performance of the broiler chicken can be remarkably improved. In the additive, lactobacillus johnsonii BS15 and enterococcus faecalis YP-2 are combined for use, and saccharomyces cerevisiae WHY-7 and bacillus amyloliquefaciens TL are combined for use, so that the average weight of the broiler chicken is increased, the spleen index after LPS stimulation is reduced, and synergistic interaction is achieved.

The invention belongs to the technical field of microorganisms, and in particular relates to enterococcus faecalis ZJUIDS-C010 with calcium enrichment capacity and application of the enterococcus faecalis ZJUIDS-C010. The invention discloses enterococcus faecalis ZJUIDS-C010, and the preservation number of the enterococcus faecalis ZJUIDS-C010 is CGMCC (China General Microbiological Culture Collection Center) NO. 34467. The invention also provides an application of the enterococcus faecalis ZJUIDS-C010 in increasing the utilization rate of calcium / enriching calcium at the same time. The enterococcus faecalis ZJUIDS-C010 can be used for preparing a product for promoting calcium absorption of intestinal tracts of pets.

The invention provides enterococcus faecalis bacteriophage vBEfaS-1017 and application thereof, the enterococcus faecalis bacteriophage vBEfaS-1017 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.46182, and the preservation time is September 6, 2024. The bacteriophage vBEfaS-1017 is high in specificity, short in incubation period and ultrahigh in stability, and has activity under the conditions of extreme pH, high temperature and ultraviolet radiation; by evaluating the in-vitro bactericidal efficacy of the bacteriophage vBEfaS-1017, it is determined that the bacteriophage vBEfaS-1017 can effectively remove a biological membrane formed by host bacteria Ef 10-17, the removal rate is as high as 90%, and the bacteriophage vBEfaS-1017 is expected to become a scavenger for solving the biological membrane. Further experiments prove that the bacteriophage vBEfaS-1017 has in-vivo and in-vitro safety. Through combined use of the bacteriophage and different antibiotics, it is found that combined use of the bacteriophage vBEfaS-1017 and the antibiotic levofloxacin can effectively treat bacteremia of mice, the treatment time after infection can be prolonged by using the bacteriophage in advance, and the bacteriophage has a good application prospect in the aspect of preventing and treating enterococcus faecalis.

A lytic agent for Enterococcus bacteria which includes a lytic enzyme or an active fragment thereof is provided. The lytic enzyme includes (a) the amino acid sequence shown in SEQ ID NO: 1, (b) an amino acid sequence in which one or multiple amino acids are added, deleted, and / or substituted in the amino acid sequence shown in SEQ ID NO: 1, or (c) an amino acid sequence having a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 1.

The invention discloses an enterococcus faecalis bacteriophage microencapsulated microsphere, and belongs to the technical field of bioengineering. The microencapsulated microsphere comprises a core material and a composite wall material, wherein the core material is enterococcus faecalis bacteriophage; the composite wall material is composed of a sodium alginate solution, a gelatin solution, a glycerin solution, a chitosan solution and a calcium chloride solution. The invention further discloses a preparation method of the microencapsulated microsphere. The preparation method comprises the steps of raw material pretreatment, mixing of a composite wall material and bacteriophage, step-by-step calcium chloride solidification, chitosan film coating and the like. The enterococcus faecalis bacteriophage microencapsulated microspheres can be used for preparing livestock and poultry breeding prevention and control preparations or feeds, the problem that free bacteriophages are poor in acid resistance and heat resistance is solved, and an efficient scheme is provided for prevention and control of enterococcus faecalis.

The invention provides a feed additive as well as a preparation method and application thereof, and belongs to the technical field of fermented feeds. The feed additive provided by the invention comprises a mixed bacterial agent, acid protease and a base material, and the mixed bacterial agent comprises bacillus subtilis YY-18 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.33097, saccharomyces cerevisiae YY-21 with the preservation number of CGMCC No.33100 and enterococcus faecalis YY-20 with the preservation number of CGMCC No.33099. According to the invention, the specific mixed bacterial agent is adopted to cooperate with the acid protease and the base material for mixed fermentation, so that the soluble protein content, the small peptide content and the viable count in the feed additive are improved, and the prepared feed additive is good in nutrition safety and high in digestible utilization rate. According to the fermented feed provided by the invention, by adding the feed additive containing the mixed microbial agent, the production performance of animals can be improved, the breeding cost is reduced, and the feed utilization rate is increased.

The application discloses a tetracycline-degrading bacterium Enterococcus faecalis SH01 and application thereof. The bacterium is separated from livestock and poultry manure and is identified as Enterococcus faecalis (Escherichia coli) Enterococcus faecalis ), and under optimal conditions for degrading TC, i.e., a temperature of 29.67 DEG C, a pH of 7.21, an inoculation amount of 1.01%, and an initial concentration of tetracycline of 39.18 mg / L ‑1 , the degradation rate can be as high as 86.04%, and the bacterium has great practical value.

The invention relates to the technical field of microbial feed additives, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the steps that lactobacillus plantarum and / or enterococcus faecalis are / is used as strains for activation and expanding culture, agricultural and sideline product waste is used as a substrate for liquid-state deep fermentation, and mature fermentation liquor is obtained; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide, granulating, and pre-drying at low temperature to obtain a semi-dry particle core; and coating with kappa-carrageenan / konjac glucomannan / phytosterol ester composite gel, drying and packaging to obtain a finished product. The double essential oils, the chitosan and the theabrownin are combined to form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; porous silicon dioxide actively maintains low water activity of particles to prevent lactic acid bacteria from being recovered in advance; the product has the advantages of high viable count, strong heat and acid resistance and excellent storage stability.

The invention discloses a compound probiotic preparation for relieving heat stress of sows and application of the compound probiotic preparation, and belongs to the technical field of animal feed. The compound probiotic preparation comprises clostridium butyricum, bacillus subtilis, bacillus licheniformis and enterococcus faecalis, wherein the final concentration of the clostridium butyricum in the feed is 1 * 10 < 6 > CFU / g, and the final concentration of the bacillus subtilis, the final concentration of the bacillus licheniformis and the final concentration of the enterococcus faecalis in the feed is 5 * 10 < 6 > CFU / g. The preparation provided by the invention can effectively reduce the stress hormone level and oxidative damage index in the blood of the sow under the heat stress condition, improve the activity of antioxidant enzyme, improve the feed intake and lactation performance in the lactation period, and reduce the backfat loss. Meanwhile, through the vertical transfer effect of the sows and the piglets, the intestinal morphology of the offspring piglets is remarkably improved, the intestinal barrier function and the antioxidant capacity are enhanced, and the weaning weight and the average daily gain of the piglets are improved, so that the negative effects of heat stress on the reproductive performance of the sows and the growth of the piglets are comprehensively relieved. The preparation is green and safe, and has important application value.

The invention discloses an anti-aging and neuroprotective effect of enterococcus faecalis SI-FC-01 based on caenorhabditis elegans, and belongs to the technical field of microbial application. The enterococcus faecalis SI-FC-01 separated from human intestinal flora is preserved in the China Center for Type Culture Collection (Wuhan University), and the preservation number of the enterococcus faecalis SI-FC-01 is CCTCC NO: M 2024091. The invention has the following beneficial effects: the study shows that the strain as a food supplement has good anti-aging, life-prolonging and neuroprotective effects on caenorhabditis elegans, is safe and free of toxic and side effects on caenorhabditis elegans, and can be used for preparing or developing anti-aging, neuroprotective and antibacterial products so as to be applied to delaying senescence, prolonging life and protecting neurological diseases. The strain has good practical application value, and an excellent basic strain is provided for further research and development of microbial anti-aging products.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to traditional Chinese medicine extract gel for promoting skin wound healing and resisting infection and a composition. The traditional Chinese medicine extract gel is prepared from a traditional Chinese medicine extract and a gel matrix according to a certain mass ratio, wherein the traditional Chinese medicine extract is a water extract of rhizoma bletillae and radix notoginseng. The traditional Chinese medicine extract gel can be independently used or further combined with a phage composition consisting of a phage composition such as a pseudomonas aeruginosa phage, a staphylococcus aureus phage, an escherichia coli phage and an enterococcus faecalis phage for use, and is used for developing products for promoting skin wound healing and resisting infection. The method can also be used for developing antibacterial products aiming at mixed bacteria such as pseudomonas aeruginosa, staphylococcus aureus, escherichia coli and enterococcus faecalis, and replacing antibiotics to be used for preventing and treating diseases caused by the mixed bacteria.

The invention relates to a genetically engineered bacterium for producing (-)-limonene, (-)-carvol and (-)-carvone and application of the genetically engineered bacterium. According to the method, endogenous ATP-citrate lyase genes ACL and CarYB genes are knocked out, endogenous mevalonic acid pathway key genes HMGR, ACCT and IDI are overexpressed, a mevalonic acid kinase gene MmMK sourced from methanosarcina martensii and mevalonic acid pathway key genes EfMvaS and EfMvaE sourced from enterococcus faecalis are introduced, and the (-)-limonene, (-)-carvol and (-)-carvone are synthesized into the genetically engineered strain. The method is realized in a platform strain for regulating and controlling the balance of the cofactor NADPHP. And the yield of the (-)-limonene in a bioreactor of 500-mL reaches 608.5 mg / L. The yield of the (-)-carveol reaches 667.6 mg / L when the (-)-carveol is fermented in a 500-mL bioreactor. The fermentation yield of the recombinant strain for producing the (-)-carvone in a 500-mL bioreactor reaches 67.1 mg / L.