131 results about "Enterococcus fecalis" patented technology

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and/or Escherichia coli and/or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and/or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The present invention provides a microbial fermented composite antibiotic-free feed containing natural antibacterial components. The feed comprises a fermented product based on the united segmented fermentation of aerobic-anaerobic composite strains, composite natural antibacterial components, a non-plant source protein/polypeptide compound nutrient supplement agent, and an additive comprising atleast a soybean protein amino acid/polypeptide compound chelate. The composite strains comprises at least lactic acid bacteria, bacillus subtilis, yeast selected from candida utilis or saccharomyces cerevisiae, and enterococcus faecalis. The fermented product based on the united segmented fermentation is prepared by carrying out secondary fermentation on fermentation raw materials through the aerobic-anaerobic composite strains. The combination of the microbial fermented feed and the natural antibacterial components is beneficial to the balance of intestinal flora of animals, and can prevent the dysbacteriosis of digestive tracts, improve the immunity functions of animal organisms, reduce or replace the use of feed antibiotics, and significantly improve the digestion and absorption efficiency of the feed and the meat quality of animals.

The invention discloses a special complex microbial agent for harmless treatment of livestock and poultry excrements. The special complex microbial agent is prepared from the following raw materials in parts by weight: 0.02-0.04 part of bacillus mucilaginosus, 0.01-0.03 part of bacillus licheniformis, 0.01-0.03 part of streptomyces jingyangensis whey powder, 0.01-0.02 part of azotobacter vinelandii, 0.04-0.08 part of aspergillus spore powder, 0.01-0.03 part of streptococcus thermophilus, 0.01-0.02 part of enterococcus faecalis, 0.02-0.04 part of antimicrobial peptide thallus, 80.0-120.0 parts of dried silkworm dropping, 2.0-6.0 parts of peanut cakes, 2.0-3.0 parts of bran, 7.0-8.0 parts of starch, 3.0-3.5 parts of molasses, 2.0-3.0 parts of monocalcium phosphate, and 1.0-1.4 parts of urea. Used for harmless treatment of livestock and poultry excrements, the special complex microbial agent has the advantages that the fermentation time is short, the quality of a fertilizer is high, the active rate of microorganisms in soil can be activated, the temperature permeability of air in soil can be increased, and the special complex microbial agent has an effect of preventing and controlling various fungal diseases and bacterial diseases, as well as effects of increasing the yield and improving the quality while providing complete nutrients for the crop, thus having better economic benefits, social benefits and ecological benefits.

The invention discloses a universal feed starter composed of the following raw materials by weight: 20-30 parts of soybean meal, 20-30 parts of corn, 20-30 parts of straw, 10-20 parts of fish meal, 10-20 parts of bran, 5-10 parts of sugar, 5-10 parts of Omega-3 fatty acid, 100 parts of multidimensional composite enzyme bacteria and 50-100 parts of water; the multidimensional composite enzyme bacteria contains 5-10 parts of bcillus subtilis, 5-1 parts of bacillus licheniformis, 10-20 parts of acetobacter aceti, 10-20 parts of lactobacillus amylovorus, 5-10 parts of candida, 10-20 parts of protease, 5-10 parts of geotrichum candidum, and balance of enterococcus faecalis. The efficient feed starter prepared by fermentation of various raw materials by the multidimensional composite enzyme bacteria can be widely used in breeding of ruminant animals, swine, poultry, aquatics and the like. The efficient feed starter fermentation agent belongs to a micro ecological preparation, does not contain any chemical agents and hormones, and is used in the production of green livestock products and aquatic products beneficial to human health.

The invention provides isolated polypeptide and nucleic acid sequences derived from Enterococcus faecalis that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.

The invention relates to a pig feed additive with efficacies of protecting intestinal tracts and improving a food calling function. The pig feed additive is characterized by being prepared from the following raw materials in parts by weight: 0.5-0.9 part of glycine zinc, 0.6-1.2 parts of beta-mannase, 0.5-1 part of yeast powder, 0.5-0.8 part of bifidobacterium bifidum probiotic powder, 0.5-0.8 part of enterococcus faecalis powder, 8-12 parts of dry white wine grain stillage and 9-12 parts of a food calling composition, wherein the composition is prepared from the following raw materials in parts by weight in a mixing manner: 10-12 parts of robinia flowers, 9-12 parts of orange peel and 1-3 parts of rapeseeds. The additive is capable of effectively adjusting the balance of intestinal flora, increasing the palatability, improving the growth performance, restraining and sterilizing bacteria, preventing and treating diarrhea and dysentery, obviously improving the animal immunity and the production performance, and reducing the ammonia-nitrogen emission to the environment.

The invention relates to an anti-stress disease-prevention piglet feed comprising the following components in parts by weight: 60-68 parts of corn flour, 5-8 parts of potato four, 3-6 parts of yellow mealworm powder, 2-5 parts of fish meal, 3-5 parts of bone meal, 2-4 parts of enterococcus faecalis, 0.5-1 part of selenium yeast, 5-8 parts of dry sweet potato vine, 8-15 parts of grape skins, 1-3 parts of citric acid, 1-3 parts of anhydrous dextrose and 2-5 parts of Chinese medicinal herb powder. Compared with the prior art, the components and the dosages are reasonably collocated to integrate the synergistic action among the components, improve the palatability of the feed, improve the food intake of piglets and the utilization ratio of the feed, reduce the breeding cost and enhance the immunologic function of the piglets at the same time, so that diseases are reduced, adverse stress responses to the outside of swine herds are reduced, the swine herds are promoted to grow quickly and healthily, the excrement off odor is reduced, and the anti-stress disease-prevention piglet feed is a green piglet feed adaptive to intensive pig production.

The invention relates to a pig-feed additive with a function of improving the immunologic function. The pig-feed additive comprises the following materials with specific proportion: glucose oxidase, alpha-hydracrylic acid, lactobacillus bulgaricus powder, enterococcus faecalis powder, egg yolk immunoglobulin, zinc glycinate, dry-beer vinasse, poplar flowers, orange peel, hawthorn, pine needle powder, ginkgo leaves, folium cortex eucommiae, alfalfa, purple perilla and the like. The pig-feed additive has the effects of invigorating the stomach and promoting digestion, clearing heat and removing toxicity, killing insects and resisting viruses, and improving the immunologic function for pigs, and has strong disease-resisting effect.

The invention discloses a compound micro-ecological preparation for aquaculture, and belongs to the field of micro-ecological preparations for aquaculture technologies. The compound micro-ecological preparation is prepared from compound microorganisms, a complex enzyme system and composite immunity enhancing factors, wherein the compound microorganisms comprise enterococcus faecalis, enterococcusfaecium, bacillus subtilis and saccharomyces cerevisiae, the complex enzyme system comprises protease, cellulase and lipase, and the composite immunity enhancing factors comprise mannan oligosaccharide, beta-glucan, chitosan oligosaccharide and peptidoglycan. The compound micro-ecological preparation has the advantages that the health problems of intestines and stomach in the existing breeding process can be effectively solved, the immunity and the disease-resistant and anti-stress capabilities can be enhanced, and green and healthy breeding can be protected.

The invention provides a liquid bacterial liquid for fermenting a piglet liquid feed, and a preparation method and application of the piglet liquid fermented feed. The liquid bacterial liquid for fermenting the piglet liquid feed comprises a bacillus liquid and a lactic acid bacteria liquid; the bacillus liquid is a mixed bacteria liquid of bacillus licheniformis and bacillus subtilis; and the lactic acid bacteria liquid is a mixed bacteria liquid of lactobacillus plantarum and enterococcus faecalis. The piglet liquid fermented feed is a feed fermented by adopting the liquid bacterial liquid for fermenting the piglet liquid feed. The liquid bacterial liquid provided by the invention can improve the utilization rate of the feed, improve the flavor of the feed and improve the palatability, and after the feed is fermented, the contents of reducing sugar and lactic acid are obviously increased, the probiotic number is obviously increased, and the feed has acid fragrance. The piglet liquidfermented feed contains a large amount of probiotics, and is mainly used for enhancing the immune function of the piglets, killing pathogenic microorganisms and preventing diseases such as diarrhea ofthe piglets by fermenting to generate lactic acid and secrete some antibacterial substances.

The invention discloses an Enterococcus faecalis strain and its application in bioelectricity production. The strain is characterized in that it is Enterococcus faecalis Z5, which is preserved by China Center for Type Culture Collection (called CCTCC for short) on November 6, 2012 and has a preservation number of CCTCC NO: M2012445. When the strain is inoculated into a microbial fuel cell, it can degrade organic matters and generate electricity simultaneously, but probably does not transmit electrons by secreting its electron transmission intermediates. The Enterococcus faecalis Z5 strain has very strong electrochemical activity, and also can use multiple types of organic matters as the sole carbon source for electricity generation. Based on the above characteristics, the strain has very good application prospects in environmental pollution remediation and bio-energy recovery.

The invention relates to a (6R, 7R)-3-[(acetyloxy) methyl]-7-[alpha-(N, N'-dicyclohexyl amidine thio)-acetylamino]-8-oxo-5-thia-1-azabicyclo [4, 2, 0]-oct-2-ene-2-carboxylic acid, a crystal thereof, and a preparation method and application thereof. Under alkaline conditions, 7-ACA reacts with bromoacetyl bromide to obtain a 7-bromo acetamino-3-[(acetyloxy) methyl]-8-oxo-5-thia-1-azabicyclo [4.2.0] oct-2-ene-2 formic acid intermediate A; and the intermediate A reacts with N, N '- dicyclohexyl thiourea to obtain the compound provided by the invention. The compound is further refined to obtain the crystal with high purity and good stability of the compound provided by the invention. The compound and the crystal thereof have antibacterial effect on Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae and Enterococcus faecalis, and are potential antimicrobial drugs with promising application prospect.

The invention relates to a lamb fourth stomach glycoprotein extraction, separation and purification method and application thereof. The lamb fourth stomach glycoprotein extraction, separation and purification method comprises the following steps that a pretreated fresh lamb fourth stomach is degreased, and ultrasonic-assisted extraction is carried out on a sodium chloride solution; centrifuging is carried out to take a supernatant; vacuum concentration and alcohol precipitation are carried out; free protein is removed with a Sevage reagent; freeze-drying is carried out to obtain crude glycoprotein powder; the glycoprotein is obtained through gel and anion exchange column chromatography separation and purification, the yield is 1.67%, the total sugar content is 70.24%, the protein content is 16.39%, and the uronic acid content is 4.12%; and purifying is carried out by HPLC to obtain the high-purity lamb fourth stomach glycoprotein, and the purity reaches 99.894%. In-vitro probiotic activity experiments prove that the lamb fourth stomach glycoprotein has the effect of promoting the growth of intestinal probiotics of bifidobacterium adolescentis, lactobacillus delbrueckii subspecies bulgaricus and enterococcus faecalis. The lamb fourth stomach glycoprotein extraction, separation and purification method has the advantages of simple process, convenience in operation, high extraction rate, high purity, stable property, no need of using a large amount of toxic solvents, low cost, easiness in popularization and application and the like.

The invention provides a traditional Chinese medicine composition for young poultry, a liquid-state fermented traditional Chinese medicine preparation and a preparation method thereof. The compositionis prepared from 30-38 parts of Astragalus membranaceus, 22-28 parts of rhizoma atractylodis macrocephalae, 20-25 parts of poria cocos, 22-28 parts of hawthorn and 18-25 parts of dandelions. The preparation comprises a composition and probiotics, wherein the probiotics are compound bacteria of clostridium butyricum, bacillus licheniformis and enterococcus faecalis. The preparation method comprises the step that the traditional Chinese medicine composition is prepared through probiotic fermentation. The traditional Chinese medicine preparation not only can increase the number of lactobacillusand bifidobacterium in intestinal tracts of young poultry to reduce the number of escherichia coli in the intestinal tracts and reduce the occurrence rate of diarrhea of the young poultry, but also can avoid the problem of high death and culling rate of the young poultry caused by abuse of antibiotics.

The invention provides a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, and relates to the field of fermented traditional Chinese medicine preparations. The fermented traditional Chinese medicine microecological preparation is prepared by fermenting a traditional Chinese medicine composition and compound microorganisms by a modern fermentation technology. The traditional Chinese medicine composition is prepared from six traditional Chinese medicinal materials including radix astragali, liquorice roots, radix rubiae, purslane herb, fructus alpiniae oxyphyllae and poplar flowers, and the compound microorganisms comprise clostridium butyricum, bacillus subtilis, lactobacillus acidophilus, bacillus licheniformis and enterococcusfaecalis. Different microorganisms are reasonably proportioned, the number of beneficial microorganisms in intestinal tracts of piglets can be increased, intestinal mucosa repair is facilitated, meanwhile, traditional Chinese medicinal materials are fermented through compound microorganisms, effective components and active substances of the traditional Chinese medicinal materials can be extractedand utilized to the maximum extent, and the absorption rate of the piglets is increased. The fermented traditional Chinese medicine microecological preparation can effectively prevent and treat pigletdiarrhea and significantly reduce mortality, has a positive effect on weight gain of the piglets, and promotes growth and development of the piglets.

The invention designs a feed additive for improving quality of eggshells in later laying period and a preparation method thereof and belongs to the field of feed additives. The feed additive is used for solving the problems of quality of eggshells in later laying period and production performance and contains the following raw materials: 15-20 parts of fructo-oligosaccharide, 20-30 parts of fermented folium cortex eucommiae, 15-20 parts of bacillus subtilis, 4-5 parts of enterococcus faecalis, 8-10 parts of sodium bicarbonate, 2-2.5 parts of vitamin C, 1.5-2 parts of zinc, 0.1-0.3 part of copper, 1-1.5 parts of manganese and 10-15 parts of zeolite powder. The invention adopts a compound nutrition technology for comprehensively increasing health level of laying hens and use ratio of calcium, phosphate and the like, promoting anti-stress capacity of the laying hens in later laying period, increasing eggshell thickness and eggshell strength, reducing loss cost of eggshells, relieving thedecreasing of laying rate, increasing egg weight and promoting production performance of laying hens.

The invention relates to application of a substitutive 2,3-didetonindoline compound in preparing bacterial infection preventing and/or treating medicines. Bacteria can be gram-positive bacteria, specifically, enterococcus faecalis and/or staphylococcus aureus. Pharmacodynamic tests show that the substitutive 2,3-didetonindoline compound has an obvious inhibiting function on virulence factors of the gram-positive bacteria, also has a regulating function on innate immune systems of caenorhabditis elegans, and can become a quite excellent candidate medicine for preventing and/or treating bacterial infection.

The invention provides a LAMP (Loop-Mediated Isothermal Amplification) primer group, a micro-fluidic chip and a kit for detecting reproductive tract pathogenic microorganisms, and belongs to the technical field of reproductive tract infection detection. The LAMP primer group comprises a streptococcus agalactiae detection primer group, an enterococcus faecalis primer group, a gardnerella vaginosisprimer group, a Candida albicans primer group and a Chlamydia trachomatis primer group. The above LAMP primer group is fixed on the micro-fluidic chip. The LAMP primer group, the micro-fluidic chip and the kit can quickly, sensitively and accurately detect streptococcus agalactiae, enterococcus faecalis, gardnerella vaginosis, Candida albicans and Chlamydia trachomatis by high repeatability.

The invention relates to the field of microorganisms and fermented feed, in particular to enterococcus faecalis and application thereof. The enterococcus faecalis is specifically (Enterococcus faecalis) PF95, is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 2020852, has the stress resistance such as temperature resistance, salt resistance and acid and alkali resistance, and can effectively inhibit growth or generation of harmful infectious microbes; the content of lactic acid is increased, and meanwhile a large number of probiotics are generated; particularly, the quality of the feed can still be well improved in the unsealing stage when the feed is opened for feeding after fermentation; a lactobacillus agent is added in the perinatal period and the lactation period for feeding, so that the breeding performance and re-breeding performance of sows can be improved, the serum indexes of the sows are optimized, and meanwhile, the growth performance of suckling piglets can be improved; and by adding the mixed lactobacillus agent for feeding, the feed intake and the growth speed of piglets can be effectively increased, and the diarrhea rate and the case fatality rate of the piglets are reduced.

The invention relates to a method for screening and acclimating high-temperature resistant enterococcus faecalis. The comprises the following steps of 1) pretreating enterococcus faecalis lawn; and 2)selecting dominant bacterial colonies from the step 1), and carrying out multiple times of ultimate temperature liquid culture and flat plate bacterial colony screening to obtain high temperature resistant enterococcus faecalis, wherein, in step 2), the ultimate temperature is 49-51 DEG C. Compared with the prior art, heat-resistant individuals are screened by an ultimate temperature culture method and a flat plate bacterial colony screening method, the heat-resistant hereditary property of the individuals is stabilized by an ultimate temperature repeated subculture method, finally a bacterial strain is acclimated to obtain the heat resistance; the survival rate of the bacterial strain in the spray drying production process is improved and the production cost is reduced. The method has simple operation procedure and good repeatability, can greatly improve the survival rate of the enterococcus faecalis spray drying, and promote the production and application of the enterococcus faecalis.