35 results about "Enterococcus fecalis" patented technology

The invention relates to the technical field of biological breeding, and discloses a composite microecological preparation for adjusting lamb intestinal flora and relieving weaning stress, the composite microecological preparation comprises, by weight, 10-40 parts of enterococcus faecalis powder, 10-40 parts of bacillus subtilis powder and 10-40 parts of saccharomycetes powder, the enterococcus faecalis powder is prepared through a gradient acid-resistant pre-domestication system, and the bacillus subtilis powder is prepared through a gradient acid-resistant pre-domestication system. The bacillus subtilis powder is prepared through an oxygen-controlled induced spore strengthening process, the saccharomycetes powder is prepared through a beta-glucan strengthening reactor, and the three kinds of bacterium powder are mixed under the sterile condition to obtain the bacillus subtilis powder, the saccharomycetes powder and the beta-glucan strengthening reactor. The tolerance, activity and stability of the strain are improved, the controllable and repeatable preparation process of the compound microecological preparation is ensured, and a reliable technical basis is provided for regulating intestinal flora of weaned lambs and relieving weaning stress.

An object of the present invention is to provide a new lytic agent capable of lysing Enterococcus bacteria. Provided is a lytic agent for Enterococcus bacteria, consisting of a lytic enzyme or an active fragment thereof, the lytic enzyme comprising: (a) the amino acid sequence shown in SEQ ID NO: 1, (b) an amino acid sequence in which one or multiple amino acids are added, deleted, and / or substituted in the amino acid sequence shown in SEQ ID NO: 1, or (c) an amino acid sequence having a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 1.

The present application provides a kind of complex probiotic paste, which combines Enterococcus faecalis, Saccharomyces cerevisiae and vitamin B 12 Producing lactic acid bacteria of canine source, adds prebiotics, flavoring substances and auxiliary substances such as paste matrix preparation.In the paste, the viable count of Enterococcus faecalis, Saccharomyces cerevisiae and lactic acid bacteria is 1-2:1-2:1-8;Paste matrix includes fructooligosaccharide 0.5-3%, psyllium husk powder 0.1-2%, goat milk powder 2-8%, chicken liver powder 1-5%, potassium sorbate 0.5-2%, xanthan gum 1-4%, and the balance is water.The above-mentioned complex probiotic paste can be stably stored for 56 days at 4 DEG C and normal temperature dry place, has good storage stability, good safety, and can adjust intestinal flora and increase the content of intestinal lactic acid bacteria.The above-mentioned complex probiotic paste has good palatability and oral tolerance for pets such as dogs and cats, and has good therapeutic effect on DSS-induced chronic enteritis diarrhea and vitamin B 12 Deficiency.

The present application relates to the technical field of microbial feed additive, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the following steps: taking Lactobacillus plantarum and / or Enterococcus faecalis as a bacterial strain to activate and expand culture, taking agricultural and sideline product waste as a substrate to obtain mature fermentation liquor through deep liquid fermentation; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide to obtain a semi-dry granule core through granulation and low-temperature pre-drying; and coating the semi-dry granule core with a κ-carrageenan / konjac glucomannan / plant sterol ester composite gel, and drying and packaging to obtain a finished product.The double essential oil, chitosan and theabrownin jointly form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; the porous silicon dioxide actively maintains low water activity of the granule to prevent the lactic acid bacteria from recovering in advance; and the product has high viable count, strong heat and acid resistance, and excellent storage stability.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The application belongs to the technical field of biotechnology, and provides application of an engineered Enterococcus faecalis capable of expressing human phenylalanine hydroxylase in a medicine for treating phenylketonuria, a plasmid with a human-derived phenylalanine hydroxylase gene after codon optimization of the Enterococcus faecalis is introduced into the Enterococcus faecalis, so that the human phenylalanine hydroxylase is expressed in the intestinal tract. Experimental results show that the human phenylalanine hydroxylase is successfully expressed in the Enterococcus faecalis through genetic engineering technology; the engineered Enterococcus faecalis can significantly metabolize phenylalanine in an in-vitro culture medium system; meanwhile, the engineered Enterococcus faecalis can significantly treat phenylketonuria, and can reduce plasma phenylalanine of a phenylketonuria suckling mouse by 46.8% after 4 weeks of treatment. It is indicated that the engineered Enterococcus faecalis has an outstanding therapeutic effect in preventing and / or treating phenylketonuria.

The invention discloses a broiler feed additive containing chlorella and application of the broiler feed additive. The additive contains chlorella; the feed also contains one or more of lactobacillus johnsonii, enterococcus faecalis, saccharomyces cerevisiae and bacillus amyloliquefaciens. By adding the additive disclosed by the invention into daily ration, the intestinal tissue form can be improved by regulating antioxidant and immune functions, so that the growth performance of the broiler chicken can be remarkably improved. In the additive, lactobacillus johnsonii BS15 and enterococcus faecalis YP-2 are combined for use, and saccharomyces cerevisiae WHY-7 and bacillus amyloliquefaciens TL are combined for use, so that the average weight of the broiler chicken is increased, the spleen index after LPS stimulation is reduced, and synergistic interaction is achieved.

The invention belongs to the technical field of microorganisms, and in particular relates to enterococcus faecalis ZJUIDS-C010 with calcium enrichment capacity and application of the enterococcus faecalis ZJUIDS-C010. The invention discloses enterococcus faecalis ZJUIDS-C010, and the preservation number of the enterococcus faecalis ZJUIDS-C010 is CGMCC (China General Microbiological Culture Collection Center) NO. 34467. The invention also provides an application of the enterococcus faecalis ZJUIDS-C010 in increasing the utilization rate of calcium / enriching calcium at the same time. The enterococcus faecalis ZJUIDS-C010 can be used for preparing a product for promoting calcium absorption of intestinal tracts of pets.

The invention provides enterococcus faecalis bacteriophage vBEfaS-1017 and application thereof, the enterococcus faecalis bacteriophage vBEfaS-1017 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.46182, and the preservation time is September 6, 2024. The bacteriophage vBEfaS-1017 is high in specificity, short in incubation period and ultrahigh in stability, and has activity under the conditions of extreme pH, high temperature and ultraviolet radiation; by evaluating the in-vitro bactericidal efficacy of the bacteriophage vBEfaS-1017, it is determined that the bacteriophage vBEfaS-1017 can effectively remove a biological membrane formed by host bacteria Ef 10-17, the removal rate is as high as 90%, and the bacteriophage vBEfaS-1017 is expected to become a scavenger for solving the biological membrane. Further experiments prove that the bacteriophage vBEfaS-1017 has in-vivo and in-vitro safety. Through combined use of the bacteriophage and different antibiotics, it is found that combined use of the bacteriophage vBEfaS-1017 and the antibiotic levofloxacin can effectively treat bacteremia of mice, the treatment time after infection can be prolonged by using the bacteriophage in advance, and the bacteriophage has a good application prospect in the aspect of preventing and treating enterococcus faecalis.

The invention discloses an enterococcus faecalis bacteriophage microencapsulated microsphere, and belongs to the technical field of bioengineering. The microencapsulated microsphere comprises a core material and a composite wall material, wherein the core material is enterococcus faecalis bacteriophage; the composite wall material is composed of a sodium alginate solution, a gelatin solution, a glycerin solution, a chitosan solution and a calcium chloride solution. The invention further discloses a preparation method of the microencapsulated microsphere. The preparation method comprises the steps of raw material pretreatment, mixing of a composite wall material and bacteriophage, step-by-step calcium chloride solidification, chitosan film coating and the like. The enterococcus faecalis bacteriophage microencapsulated microspheres can be used for preparing livestock and poultry breeding prevention and control preparations or feeds, the problem that free bacteriophages are poor in acid resistance and heat resistance is solved, and an efficient scheme is provided for prevention and control of enterococcus faecalis.

The application discloses a tetracycline-degrading bacterium Enterococcus faecalis SH01 and application thereof. The bacterium is separated from livestock and poultry manure and is identified as Enterococcus faecalis (Escherichia coli) Enterococcus faecalis ), and under optimal conditions for degrading TC, i.e., a temperature of 29.67 DEG C, a pH of 7.21, an inoculation amount of 1.01%, and an initial concentration of tetracycline of 39.18 mg / L ‑1 , the degradation rate can be as high as 86.04%, and the bacterium has great practical value.

The invention relates to the technical field of microbial feed additives, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the steps that lactobacillus plantarum and / or enterococcus faecalis are / is used as strains for activation and expanding culture, agricultural and sideline product waste is used as a substrate for liquid-state deep fermentation, and mature fermentation liquor is obtained; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide, granulating, and pre-drying at low temperature to obtain a semi-dry particle core; and coating with kappa-carrageenan / konjac glucomannan / phytosterol ester composite gel, drying and packaging to obtain a finished product. The double essential oils, the chitosan and the theabrownin are combined to form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; porous silicon dioxide actively maintains low water activity of particles to prevent lactic acid bacteria from being recovered in advance; the product has the advantages of high viable count, strong heat and acid resistance and excellent storage stability.

The invention discloses a compound probiotic preparation for relieving heat stress of sows and application of the compound probiotic preparation, and belongs to the technical field of animal feed. The compound probiotic preparation comprises clostridium butyricum, bacillus subtilis, bacillus licheniformis and enterococcus faecalis, wherein the final concentration of the clostridium butyricum in the feed is 1 * 10 < 6 > CFU / g, and the final concentration of the bacillus subtilis, the final concentration of the bacillus licheniformis and the final concentration of the enterococcus faecalis in the feed is 5 * 10 < 6 > CFU / g. The preparation provided by the invention can effectively reduce the stress hormone level and oxidative damage index in the blood of the sow under the heat stress condition, improve the activity of antioxidant enzyme, improve the feed intake and lactation performance in the lactation period, and reduce the backfat loss. Meanwhile, through the vertical transfer effect of the sows and the piglets, the intestinal morphology of the offspring piglets is remarkably improved, the intestinal barrier function and the antioxidant capacity are enhanced, and the weaning weight and the average daily gain of the piglets are improved, so that the negative effects of heat stress on the reproductive performance of the sows and the growth of the piglets are comprehensively relieved. The preparation is green and safe, and has important application value.

The invention relates to a genetically engineered bacterium for producing (-)-limonene, (-)-carvol and (-)-carvone and application of the genetically engineered bacterium. According to the method, endogenous ATP-citrate lyase genes ACL and CarYB genes are knocked out, endogenous mevalonic acid pathway key genes HMGR, ACCT and IDI are overexpressed, a mevalonic acid kinase gene MmMK sourced from methanosarcina martensii and mevalonic acid pathway key genes EfMvaS and EfMvaE sourced from enterococcus faecalis are introduced, and the (-)-limonene, (-)-carvol and (-)-carvone are synthesized into the genetically engineered strain. The method is realized in a platform strain for regulating and controlling the balance of the cofactor NADPHP. And the yield of the (-)-limonene in a bioreactor of 500-mL reaches 608.5 mg / L. The yield of the (-)-carveol reaches 667.6 mg / L when the (-)-carveol is fermented in a 500-mL bioreactor. The fermentation yield of the recombinant strain for producing the (-)-carvone in a 500-mL bioreactor reaches 67.1 mg / L.

An immunomodulatory material and its preparation method. Said material comprises fermented rice bran and heat-killed Enterococcus faecalis, which concentration is not less than 5x 10 9 / g. Said material is prepared by cultivation of E. faecalis in a culture medium containing fermented rice bran and followed heat-sterilization.

The present invention provides combinations of microbial flora selected from the group consisting of Phascolactobacterium faecium, Phascolactobacterium succinatus, Dialister succinatus, Dialister propionysifaciens, Bifidobacterium bifidum, and Lactobacillus intestinalis for use in succinate consumption, processes for producing such combinations, their compositions, and uses.

The present application relates to the technical field of bioengineering, and particularly relates to a recombinant Saccharomyces cerevisiae and a construction method and application thereof, comprising the following steps: step one, using the whole genome of Enterococcus faecalis as a template, a recombinant strain SC1-1 is constructed; step two, using the recombinant strain SC1-1 as a primary strain, L-lactate dehydrogenase CYB2 gene of Saccharomyces cerevisiae is knocked out to obtain a recombinant strain SC2; step three, using the recombinant strain SC2 as a primary strain, branched-chain-2-oxo acid decarboxylase THI3 gene of Saccharomyces cerevisiae is knocked out to obtain a recombinant strain SC3; step four, using the whole genome of Saccharomyces cerevisiae as a template, a recombinant strain SC4 is constructed. The present application innovatively optimizes the source of L-lactate dehydrogenase L-LDH, knocks out L-lactate dehydrogenase CYB2 and branched-chain-2-oxo acid decarboxylase THI3, overexpresses pyruvate kinase CDC19 and H(+)-ATPase PMA2, and makes the L-lactic acid production of Saccharomyces cerevisiae increase to 33.45 g / L.

The invention provides cat-source enterococcus faecalis ZYpet-013 with cholesterol lowering activity and application of the cat-source enterococcus faecalis ZYpet-013. The cat-source enterococcus faecalis ZYpet-013 is derived from intestinal tracts of healthy pet cats, and the preservation number of the cat-source enterococcus faecalis ZYpet-013 is CGMCC No. 32009. The enterococcus faecalis ZYpet-013 is used for preparing products for degrading cholesterol, a cat food spraying composition stably loading the strain and a low-temperature spraying process are developed in a matched mode, the cat food with the cholesterol reducing function is prepared, through daily feeding, the levels of serum total cholesterol and triglyceride of pet cats are safely and effectively reduced, blood fat metabolism is improved, and the cholesterol reducing function is achieved. The requirements of industrial production and shelf life stability are met.

The present invention consists of a process for removing impurities from hard water. Using chemical agents, it removes impurities by mixing a chemical preparation and employing decantation in the first stage of impurity removal. Subsequent validation of the results allows for the removal of other types of impurities present in the water, such as arsenic, cadmium, cyanide, copper, chromium, mercury, nickel, lead, and zinc. Finally, it removes contaminants and microorganisms from the following group: fats and oils, suspended solids, organic carbon, total nitrogen, total phosphorus, worm eggs, E. coli, and fecal enterococci. The resulting sediments are used as biofertilizers, and the treated water can be discharged into conventional municipal sewer systems or, if necessary, treated with ozone (O3) for human consumption.

The invention discloses a biosynthesis method of alpha-lipoic acid, an engineering strain and a preparation method of the engineering strain. The construction of the engineering strain comprises the following steps: transforming lipocaprylamide enzyme Lpa of enterococcus faecalis, constructing the lipocaprylamide enzyme Lpa to an arabinose promoter or a Cold temperature-sensitive promoter, and then replacing the lipocaprylamide enzyme Lpa to a LacZ gene and an lpxM gene of escherichia coli through RED recombination; or the genes of LipD, lplA, lipA and lpA which are subjected to gene code modification are expressed in the saccharomyces cerevisiae through a pESC vector. The engineering strain is cultured to biologically synthesize alpha-lipoic acid, so that the fermentation cost is further reduced, cyclic synthesis is realized, and the yield is greatly increased.

A compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, for use in the treatment of a patient infected with pathogenic organisms; wherein X and Y are each independently selected from C, N, O and S, provided at least one of X and Y is N, O or S; wherein R1 and R2 are each independently selected from an optionally substituted C1-C8 alkyl, C1-C8 alkenyl, aryl, heteroaryl or alkylaryl group; and wherein Z is selected from H, —CN, —NO2, —NR3R4, —NR5(CO)R6; C1-C4 alkyl, C1-C4 alkoxy, —(CO)WR12, hydroxy, amino, thiol, chloro, fluoro, CF3, CHF2 or CH2F groups. The compounds may be effective in treating patients / animals infected with parasites selected from Schistosoma, Haemonchus, Eimeria, Echinococcus, Dirofilaria, Fasciola or Plasmodium parasites. Additionally or alternatively, the compounds of formula (I) may be effective in treating patients infected with pathogenic bacteria selected from S. aureus, MRSA and Enterococcus faecalis.

The invention relates to molecular glue based on protein in a DUF11 structural domain of enterococcus faecalis. The invention also relates to a nanoparticle vaccine composition formed by the molecular glue, and the immunogen is covalently displayed on the surface of the nanoparticle by the molecular glue.

The invention discloses a material with probiotics coated with a tea polyphenol-based complex, a preparation method of the material and application of the material in relieving intestinal injury induced by nano-plastics, and relates to the technical field of material science. The material for coating the probiotics with the tea polyphenol-based complex comprises a core material and a shell material at least partially coating the surface of the core material, the core material comprises enterococcus faecalis, the enterococcus faecalis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the enterococcus faecalis is CGMCC 1.10682; the shell material comprises a complex of tea polyphenol and ferric ions. The material of the tea polyphenol-based complex coated probiotics is not limited by specific nano plastic types, has the advantages of being green, safe, edible and universal, and can effectively prevent the nano plastic from damaging intestinal barriers by promoting in-situ aging and agglomeration of the nano plastic in intestinal tracts, so that the nano plastic is discharged out of the body along with excrement, and intestinal tract protection is achieved.

The invention belongs to the field of resource development and utilization of oxgall-resistant bezoar transformed bacteria, and relates to enterococcus faecalis, a xanthogenic bacteria combination, a microbial inoculum, application of the microbial inoculum and a method for cultivating bezoar. The new strain enterococcus faecalis WLK2401JZ-07 is obtained through screening, and compared with other yellow-causing bacteria, the new strain enterococcus faecalis WLK2401JZ-07 has the advantages in calculus bovis cultivation, especially in the aspects of beta-glucuronidase activity in bile in the cultivation process, the survival number of bacterial colonies, the capability of being converted and combined with bilirubin and cholic acid and the like. Enterococcus faecalis and other bile-resistant microorganisms (such as escherichia coli, bacillus, lactobacillus and the like) are combined for use, so that preparation of high-quality in-vivo cultivated calculus bovis can be stably realized, and a synergistic effect is generated. And compared with single strain fermentation, the calcium bilirubin can be efficiently produced, meanwhile, the health of cattle bodies can be guaranteed, and the method has a good application prospect in the aspect of culturing calculus bovis.

The invention provides a nano antibacterial peptide based on a leucine zipper and a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence of the nano antibacterial peptide is as shown in SEQ ID No.1. The antibacterial peptide disclosed by the invention has self-assembly capability, can be assembled to form a compact nanofiber net, has an antibacterial effect on gram-negative bacteria including escherichia coli, pseudomonas aeruginosa and salmonella typhimurium and gram-positive bacteria including staphylococcus aureus, staphylococcus epidermidis and enterococcus faecalis, and meanwhile, has relatively high biocompatibility, so that the antibacterial peptide can be used for preparing the antibacterial peptide. Moreover, the antibacterial peptide disclosed by the invention can stably exert an antibacterial effect under the conditions of serum, acid and alkali. In conclusion, the antibacterial peptide disclosed by the invention is an antibacterial peptide with relatively good antibacterial activity and biocompatibility.

The invention discloses a compound microbial agent for preparing a fermented feed, the fermented feed, a preparation method and application. The compound microbial agent is prepared from lactobacillus fermentation broth freeze-dried powder, enterococcus faecalis fermentation broth freeze-dried powder, bacillus subtilis fermentation broth freeze-dried powder, a clostridium butyricum spore preparation and aspergillus niger spore powder according to a specific weight part ratio. A mixture of complete formula feed powder and bran / rice bran is used as a fermentation substrate, and the fermented feed is prepared through anaerobic fermentation of the microbial inoculum; the fermented feed is used for replacing basic daily ration of live pigs according to the weight ratio of 5-30% for feeding, so that the digestion and absorption rate of the feed and the production performance of the live pigs can be remarkably improved, the intestinal health of the live pigs is improved, the diarrhea occurrence rate is reduced, the immunity is enhanced, and the nitrogen and phosphorus excretions of the live pigs and the production of malodorous substances in a breeding environment are reduced. According to the compound microbial agent, the functions of all strains are complementary, the synergistic effect is remarkable, a fermented feed product is safe and efficient, and an effective technical scheme is provided for healthy breeding of live pigs and sustainable development of animal husbandry.

The invention discloses a pellet feed for laboratory mice and a preparation method of the pellet feed. The feed consists of the following raw materials in percentage by weight: 42-50% of corn, 8-12% of fermented soybean meal, 4-8% of insect protein (hermetia illucens larva powder), 2-4% of maltodextrin, 1-2% of inulin, 0.5-2% of inactivated enterococcus faecalis, 1.5-3% of a vitamin premix, 3-5% of a mineral premix, 0.3-0.8% of xylooligosaccharide, 2-4% of vegetable oil, 1-3% of cellulose and the balance of auxiliary materials. The method has the advantages that heavy metal and micro-plastic pollution are avoided, allergens are removed, and the survival rate of laboratory mice is increased by 20%; active ingredients of the inactivated enterococcus faecalis are reserved through a spraying process, and the number of beneficial bacteria in the intestinal tract is increased by 25-35% compared with a contrast; the feed conversion rate is increased by 21-32%, the weight gain is stable, and the fluctuation of experimental data is reduced.

This application belongs to the field of microbial technology and discloses a low-temperature resistant Lactobacillus and Lactococcus compound inoculant and its application in oat silage. This low-temperature resistant Lactobacillus and Lactococcus compound inoculant includes Lactobacillus helveticus T3, Lactobacillus fermentum 1761, and Enterococcus faecalis CTB. This low-temperature resistant, high-performance Lactobacillus and Lactococcus compound inoculant not only maintains stable and efficient functional activity in extreme environments of 5℃ to -20℃, but also rapidly establishes an acidic environment during silage in natural environments (-20℃ to 17℃), effectively inhibiting protein degradation and putrefactive microbial activity, releasing more nutrients, improving silage stability and preservation, and further enhancing fermentation efficiency. Simultaneously, it has lower costs, good economic viability and prospects for large-scale application, providing a reliable and innovative technical solution for the efficient resource utilization of high-fiber raw materials in high-altitude and cold regions.

The present application belongs to the field of development and utilization of bile-resistant bovine gallstone conversion bacteria resources, and relates to a combination of Enterococcus faecalis and yellow-producing bacteria, a bacterial agent, an application thereof, and a method for cultivating bovine gallstones. A new bacterial species, Enterococcus faecalis WLK2401JZ-07, is screened, which has advantages in cultivating bovine gallstones compared with other yellow-producing bacteria, especially in the aspects of beta-glucuronidase activity in bile, colony survival number, conversion capacity of conjugated bilirubin and conjugated cholic acid during the cultivation process. The combination of Enterococcus faecalis and other bile-resistant microorganisms (such as Escherichia coli, Bacillus, Lactobacillus, etc.) can stably achieve the preparation of high-quality in-vivo cultivated bovine gallstones, and has a synergistic effect. Compared with single-strain fermentation, the method can efficiently produce calcium bilirubinate while ensuring the health of the bovine body, and has a good application prospect in cultivating bovine gallstones.

The invention discloses a horse-derived anaerobic strain for improving athletic ability as well as a screening method and application thereof, and relates to the field of microorganisms. The invention discloses a horse-derived anaerobic strain capable of improving athletic ability, which is preserved in China General Microbiological Culture Collection Center, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 19, 2023, and the preservation number is CGMCC No.28469. The horse-derived anaerobic strain is classified and named as Enterococcus faecalis. The invention also discloses a screening method and application of the horse-derived anaerobic strain. The horse-derived anaerobic strain for improving the athletic ability, the screening method and the application have important guiding significance on development of probiotics of racing horses and additives of microbial feeds.