Method for mixed fermentation of probiotics containing bacillus coagulans

A technology of Bacillus coagulans and micro-ecological preparations, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve the problems of single strain and large limitations in fermentation

Inactive Publication Date: 2019-03-08
焦作恒辉牧业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of microecological preparations depends on the strains used in fermentation, but in the current microecological products, the strains used for fermentation are rel

Method used

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  • Method for mixed fermentation of probiotics containing bacillus coagulans
  • Method for mixed fermentation of probiotics containing bacillus coagulans
  • Method for mixed fermentation of probiotics containing bacillus coagulans

Examples

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Example Embodiment

[0026] Example 1

[0027] Such as figure 1 As shown, a method of a mixed fermentation probiotic preparation containing Bacillus coagulans includes the following steps:

[0028] The first step is to rejuvenate and activate the strains. Inoculate the required strains of Lactobacillus bulgaricus, Saccharomyces cerevisiae, and Bacillus coagulans on the corresponding solid medium, and place them in a constant temperature incubator for twenty-four hours. Store in the refrigerator at 5℃ for later use;

[0029] The second step is to configure the liquid medium for the seed solution configuration. Firstly, the medium of Lactobacillus bulgaricus, the medium group of Saccharomyces cerevisiae, and the medium of Bacillus coagulans are configured, and then the medium of Lactobacillus bulgaricus, The culture medium group of Saccharomyces cerevisiae and the culture medium of Bacillus coagulans were sterilized in a sterilization pot. After the sterilization was completed, they were put into the ase...

Example Embodiment

[0044] Example 2

[0045] Such as figure 1 As shown, a method of a mixed fermentation probiotic preparation containing Bacillus coagulans includes the following steps:

[0046] The first step is to rejuvenate and activate the strains. Inoculate the required strains of Lactobacillus bulgaricus, Saccharomyces cerevisiae, and Bacillus coagulans on the corresponding solid culture medium, and place them in a constant temperature incubator for 24 hours. Store in the refrigerator at 3℃ for later use;

[0047] The second step is to configure the liquid medium for the seed solution configuration. Firstly, the medium of Lactobacillus bulgaricus, the medium group of Saccharomyces cerevisiae, and the medium of Bacillus coagulans are configured, and then the medium of Lactobacillus bulgaricus, The culture medium group of Saccharomyces cerevisiae and the culture medium of Bacillus coagulans were sterilized in a sterilization pot. After the sterilization was completed, they were put into the asep...

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Abstract

The invention relates to a method for mixed fermentation of probiotics containing bacillus coagulans. The method comprises the following four steps: rejuvenation and activation of a culture, seed solution configuration of a configured liquid medium, secondary culture and solid material production. On the one hand, a processing and production technology is simple, a production process is standard,the production efficiency and quality can be improved effectively, the production cost is reduced, on the other hand, the bacillus coagulans, saccharomyces cerevisiae and lactobacillus bulgaricus arefermented in a mixed manner creatively, the content and palatability of lactic acid are greatly improved, PH of a fermented finished product is between 4.0 and 4.5, and the content of the bacillus coagulans is greater than or equal to 109 CFU/mL; the content of the saccharomyces cerevisiae is greater than or equal to 108 CFU/mL; and the content of lactic acid bacteria is greater than or equal to 108 CFU/mL.

Description

technical field [0001] The invention relates to a mixed fermentation micro-ecological preparation method containing bacillus coagulans, which belongs to the field of biological technology. Background technique [0002] Microecological agents are a class of additives composed of many beneficial microorganisms and their metabolites, which can effectively promote the adjustment of intestinal microecological balance in animals. With the rapid development of animal husbandry, various disadvantages of antibiotics as feed additives are gradually recognized by people. For example, while killing harmful bacteria, it also kills beneficial microorganisms in the intestinal tract, resulting in an imbalance of microbial flora in the intestinal tract. , the drug residues in animal products will seriously affect the treatment and inheritance of human diseases, and directly affect human health. At the same time, it also constitutes a trade barrier for the export of animal products, which bri...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18A23K10/12C12R1/07C12R1/225C12R1/865
CPCA23K10/12C12N1/18C12N1/20
Inventor 曹宇
Owner 焦作恒辉牧业有限公司
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