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Plant lactobacillus and application thereof in ensilage of warm-season forage

A kind of Lactobacillus plantarum, warm-season technology, applied to Lactobacillus plantarum and its application field in warm-season pasture silage, can solve the problem of no biological additives and the like, achieve no environmental pollution, increase lactic acid content, and improve fermentation quality Effect

Inactive Publication Date: 2016-05-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of additive has been used in silage such as leguminous pasture and corn stalks, but there is still no similar biological additive suitable for warm-season pasture.

Method used

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  • Plant lactobacillus and application thereof in ensilage of warm-season forage
  • Plant lactobacillus and application thereof in ensilage of warm-season forage
  • Plant lactobacillus and application thereof in ensilage of warm-season forage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation and Identification of Lactobacillus plantarum LP1

[0028] Take 20g of natural wild grass silage in Hebei area, add 180mL of distilled water, shake it with a shaker for 30min, 10 -1 -10 -6 Gradient dilution. Take 1mL from each gradient dilution solution into a petri dish, add 10-15mL sterilized MRS solid medium, shake well, wait for it to cool and solidify, let it stand for anaerobic culture for 48h, pick the colony shape, size, For single colonies with obvious differences in color and gloss, streak repeatedly until pure colonies are obtained.

[0029] The isolated and screened strains were identified and analyzed comprehensively based on their cell morphology, physiological and biochemical characteristics, and 16SrDNA gene sequence. The strain was identified as Lactobacillus plantarum and named LP1, which is a new strain of Lactobacillus plantarum.

[0030] The biological characteristics of the strain LP1 are as follows: the LP1 strain grows wel...

Embodiment 2

[0036] Embodiment 2: Growth assay of plant lactobacillus (Lactobacillus plantarum) LP1 under different pH, temperature and salt concentration environmental conditions

[0037] Use 1mol / L hydrochloric acid and 1mol / L sodium hydroxide to adjust the initial pH value of the MRS medium to 3.0, 3.5, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0 respectively, and cultivate plant milk at a constant temperature of 37°C Rod LP124h, without acid adjustment during the cultivation process, the light absorption value at 600nm (OD600) was measured with a spectrophotometer, and the results are shown in Table 2. The results showed that Lactobacillus plantarum LP1 could grow under the condition of initial pH3.5-pH8.0.

[0038] The activated Lactobacillus plantarum LP1 was inoculated in the MRS liquid medium, cultured at 10°C, 15°C, 20°C, 30°C, 40°C and 45°C for 24 hours, and the light absorption at 600nm was measured with a spectrophotometer Value (OD600), the results are shown in Table 2. The results show th...

Embodiment 3

[0044] Embodiment 3: Plant Lactobacillus (Lactobacillus plantarum) LP1 and cellulase are used as biological additives to prepare warm-season grass silage

[0045] 1. Raw material preparation:

[0046] (1) Forage preparation: Taking elephant grass (Pennisetum purpureum Schum.) and multi-flowered ryegrass (Lolium multiflorum Lam.) as examples, the pasture is mowed from the jointing stage to the earing stage to ensure that the raw materials are clean and free of impurities such as soil and manure. As short as 2-3cm.

[0047](2) Preparation of biological additives: Lactobacillus plantarum LP1 and cellulase were activated by adding water (without disinfectant) for 20 minutes.

[0048] 2. Preparation of warm-season forage silage

[0049] Spray the activated Lactobacillus plantarum LP1 and cellulase on the treated grass, and the amount of Lactobacillus plantarum LP1 added is not less than 10 9 CFU / kg fresh grass, cellulase not less than 100U / kg fresh grass, mix well and fill immed...

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Abstract

The invention discloses a plant lactobacillus and an application thereof in the ensilage of warm-season forage, and belongs to the field of processing and storage of forage. The provided strain (LP1) is named as Lactobacillus plantarum. The preservation number of the strain in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No. 10473. The plant lactobacillus is separated from natural herbage silage and is a novel strain of plant lactobacillus. The biological additive prepared from the plant lactobacillus and cellulase can prominently improve the fermentation quality of ensilage of warm-season forage, has the advantages of high efficiency and no pollution to the environment, does not have any adverse influence on livestock, can be easily mastered during the addition process, and can be widely used in the field of warm-season forage silage preparation.

Description

technical field [0001] The invention belongs to the field of forage feed processing and storage, in particular to a plant lactobacillus and its application in warm-season forage silage. Background technique [0002] With the development of animal husbandry in our country, the demand for animal feed is getting higher and higher. Warm-season forages, such as elephant grass and sudan grass, are mostly native to Africa. They have high yield and nutritional value, high digestibility and good palatability. Cattle, sheep, and horses are all fond of eating them. They are very important sources of animal feed in my country. [0003] Warm-season pastures are mostly planted in southern my country, where it is rainy in summer, so it is difficult to prepare hay. Silage can solve the problem that hay is not suitable for preparation in the rainy season and in humid and rainy areas, and at the same time reduce the loss of leaves, rain, mildew and other losses caused by hay preparation; Si...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K20/189C12R1/25
CPCC12N1/205C12R2001/25
Inventor 玉柱张庆张英俊杨富裕
Owner CHINA AGRI UNIV
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