The invention discloses a method and a
fermentation device for increasing the production and
fermentation yield of
neomycin sulfate, and the method comprises the following steps: S1, inoculating sandy soil spores to a slant culture medium, and culturing to obtain a slant
spore suspension; s2, inoculating the slant
spore suspension into a seed tank filled with a seed culture medium, and culturing to obtain a seed solution; s3, inoculating the seed solution into a
fermentation tank filled with a fermentation basal culture medium, controlling stable fermentation, then determining
reducing sugar and
ammoniacal nitrogen, supplementing
sugar and an
ammonium sulfate solution according to the determined values, preparing resin, sterilizing and cooling for later use, and in the fermentation process, performing fermentation for 2-3 hours; adding a certain amount of resin as a supplementary material to the fermentation tank instead of the
ammonium sulfate solution at regular intervals; s4, after fermentation is completed, discharging fermentation liquor from the fermentation tank, and separating out resin; s5, measuring the total
titer generated by fermentation; according to the method,
neomycin and other substances generated in fermentation liquor are adsorbed through resin, and the yield is increased.