Preparation method and application of cation-modified glycoprotein type microorganism flocculating agent

A technology of microbial flocculant and glycoprotein, which is applied in the field of water treatment, can solve the problems of environmental secondary pollution, etc., and achieve the effect of simplifying the adding process, reducing the content, and simplifying the adding process

Active Publication Date: 2015-12-09
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Based on the problems existing in the prior art, the present invention provides a method for preparing a cationic modified glycoprotein microbial flocculant, which improves the activity of the glycoprotein microbial flocculant and solves the need for using the glycoprotein microbial flocculant in the prior art Coagulation aids cause secondary pollution of the environment by coagulation aids
[0004] Another object of the present invention is to provide an application of a glycoprotein microbial flocculant modified by the above method to solve the problem that the existing microbial flocculant water treatment process will cause secondary pollution to the environment

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] This embodiment provides a preparation method of a cationic modified glycoprotein microbial flocculant, the method comprising the following steps:

[0032] Step 1, preparing glycoprotein microbial flocculant:

[0033] Step 1.1, prepare seed solution:

[0034] Pick a ring of bacteria from the preservation slant of Klebsiella NY1 strain and insert it into beef extract peptone liquid medium for culture to obtain OD 600nm Klebsiella NY1 seed solution of 1.82 ± 0.08;

[0035] Step 1.2, Klebsiella NY1 fermentation to produce flocculant:

[0036] 100 mL of fermentation culture was sterilized at 121° C. for 30 min. Under a sterile environment, draw 2 mL of Klebsiella NY1 seed solution and add it to the fermentation medium. Under the conditions of 30°C and 160r / min, shake and cultivate at constant temperature for 72 hours to obtain the crude product of glycoprotein microbial flocculant;

[0037] Step 1.3, the purification method of the crude product of glycoprotein microbia...

Embodiment 2

[0064] This embodiment provides a preparation method of a cationic modified glycoprotein microbial flocculant, the method comprising the following steps:

[0065] Step 1, preparing glycoprotein microbial flocculant:

[0066] The specific content of step 1 is basically the same as that of Example 1, except that the stabilizing agent in step 1.3 is potassium chloride.

[0067] Step 2, cationic modification of glycoprotein microbial flocculant:

[0068] Mix 0.02mol of 3-chloro-2-hydroxypropyltrimethylammonium chloride (CTA) with NaOH solution with a concentration of 0.5mol / L at a molar ratio of 1:1.6, let it react for 10min, then add 10g of NY1 bacteria The flocculant produced is pure. The mixture was stirred at room temperature for 0.5 h, put into an incubator and allowed to react at 60° C. for 1 h to obtain a mixture of cation-modified glycoprotein microbial flocculants.

[0069] Step 3, purification of cationized glycoprotein microbial flocculant:

[0070] Completely disso...

Embodiment 3

[0086] This embodiment provides a preparation method of a cationic modified glycoprotein microbial flocculant, the method comprising the following steps:

[0087] Step 1, preparing glycoprotein microbial flocculant:

[0088] The specific content of step 1 is basically the same as that of Example 1, except that the stabilizing agent in step 1.3 is sodium sulfate.

[0089] Step 2, cationic modification of glycoprotein microbial flocculant:

[0090] Mix 0.02mol of 3-chloro-2-hydroxypropyltrimethylammonium chloride (CTA) with 1mol / L NaOH solution at a molar ratio of 1:0.8, let it react for 10min, then add 10gNY1 bacteria Produce pure flocculant. The mixture was stirred at room temperature for 2 hours, put into a thermostat to react at 60° C. for 1 hour, and a mixture of cation-modified glycoprotein microbial flocculants was obtained.

[0091] Step 3, purification of cationized glycoprotein microbial flocculant:

[0092] Completely dissolve the mixture obtained in the above ste...

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Abstract

The invention provides a preparation method and application of a cation-modified glycoprotein type microorganism flocculating agent. The preparation method comprises the following steps: mixing 3-chlorine-2-hydroxypropyl PDMC with a NaOH solution for reaction, adding a glycoprotein type microorganism flocculating agent for mixing, stirring under room temperature for 0.5-2 hours, and reacting under the temperature of 60-90 DEG C for 1-3 hours, thus obtaining the cation-modified glycoprotein type microorganism flocculating agent after purification. The flocculating agent is applied to removal of kaoline from wastewater. The cation-modified glycoprotein type microorganism flocculating agent prepared by the preparation method can effectively cationize a microorganism flocculating agent loaded with negative electricity, so that a flocculating aid used in a microorganism flocculating agent use process is omitted. In the use process, other flocculating aids are not needed, so that suspensions in kaoline simulation wastewater can be removed; therefore the adding process of the flocculating agent is simplified. The method disclosed by the invention finally achieves the effect of simplifying the flocculating agent adding technology, so that the content of inorganic flocculating acid substances in settled sludge is reduced, and secondary pollution caused by the settled sludge to the environment is alleviated.

Description

technical field [0001] The invention belongs to the field of water treatment and relates to a microbial flocculant, in particular to a preparation method and application of a cationic modified glycoprotein microbial flocculant. Background technique [0002] Microbial flocculant is a kind of flocculant researched and developed in the late 1980s. It is a kind of metabolites produced by microorganisms with flocculant activity, mainly including glycoprotein, polysaccharide, protein, cellulose and DNA, and bacteria with flocculant activity. body etc. It has been widely studied because of its advantages of high efficiency, non-toxicity, no secondary pollution and wide range of uses. However, the currently produced microbial flocculants are all negatively charged, and the Zeta potential is between -50mV and -80mV. Normally, Ca is required to use 2+ , Mg 2+ Cationic or polyaluminum chloride can be used as a coagulant to improve its activity. When the kaolin in water is 2.5-5g / L...

Claims

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Application Information

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IPC IPC(8): C02F3/34
Inventor 聂麦茜聂红云
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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