Method for improving fermentation yield in production of neomycin sulfate and fermentation device

Abstract

The invention discloses a method and a fermentation device for increasing the production and fermentation yield of neomycin sulfate, and the method comprises the following steps: S1, inoculating sandy soil spores to a slant culture medium, and culturing to obtain a slant spore suspension; s2, inoculating the slant spore suspension into a seed tank filled with a seed culture medium, and culturing to obtain a seed solution; s3, inoculating the seed solution into a fermentation tank filled with a fermentation basal culture medium, controlling stable fermentation, then determining reducing sugar and ammoniacal nitrogen, supplementing sugar and an ammonium sulfate solution according to the determined values, preparing resin, sterilizing and cooling for later use, and in the fermentation process, performing fermentation for 2-3 hours; adding a certain amount of resin as a supplementary material to the fermentation tank instead of the ammonium sulfate solution at regular intervals; s4, after fermentation is completed, discharging fermentation liquor from the fermentation tank, and separating out resin; s5, measuring the total titer generated by fermentation; according to the method, neomycin and other substances generated in fermentation liquor are adsorbed through resin, and the yield is increased.

Description

technical field [0001] The invention relates to the technical field of neomycin sulfate fermentation, in particular to a method and a fermentation device for improving the production and fermentation yield of neomycin sulfate. Background technique [0002] Neomycin produced by fermentation of Streptomyces freundii has a strong antibacterial effect on most Gram-negative bacteria such as Escherichia coli, Proteus, Salmonella and Pasteurella multocida. It has the advantages of strong antibacterial ability, wide range of action, less dosage, low price, no side effects, no allergic reaction when taken orally, high safety, and is not easy to produce drug resistance and cross-resistance, so it has become a commonly used veterinary drug at home and abroad. [0003] During the fermentation process of neomycin, it is easy to produce substances that are toxic to the production bacteria, such as end products and intermediate metabolites, so that the metabolism of the production bacteria...

AI Technical Summary

Problems solved by technology

[0003] During the fermentation process of neomycin, it is easy to produce substances that are toxic to the production bacteria, such as end products and intermediate metabolites, so that the metabolism of the production bacteria is subject to feedback inhibition, thereby limiting the fermentation output and keeping the cost high

At present, in the fermentation process of gentamicin and punamycin, there are patents for adding resin to solid-liquid two-phase fermentation to increase the potency. However, the resin is directly poured into the fermentation broth, and a certain amount of water needs to be put in every once in a while. Resin, when it is necessary to separate the resin in the fermentation broth, it is also necessary to use a screen to separate the resin in the fermentation broth. Therefore, during the entire fermentation process, the process of adding and separating the resin is complicated and time-consuming, which affects the entire fermentation efficiency.

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