Fermentation device and method for efficiently fermenting and producing bacteriocin

A fermentation device and a bacteriocin technology are applied in the field of fermentation separation coupling process for producing bacteriocin, which can solve the problems of inability to influence the yield of Nisin, difficulty in realizing industrialized production, and high separation cost, so as to relieve the feedback inhibition of bacterial production and metabolism, Improve application efficiency and simplify the effect of downstream purification processes

Active Publication Date: 2017-05-31
昆山博青生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Pongtharang ku T et al. [PONGTHARANGKU T, Appl.Microbiol.Biot echnol., 2007,74:555-562] studied the production process of nisin coupled with microbial fermentation and membrane separation and adsorption separation. The results showed that compared with the uncoupled process, the coupled The process increases the titer of nisin from 1897IU / mL to 7445IU / mL, the effect is very significant, but the process is complicated, the cost of separation is high, and it is difficult to realize industrial production
There is a foam separation method in the Nisin extraction method. In this method, 0.1% Tween-80 is first added to the fermentation broth, and then aerated from the bottom of the fermentation broth to generate a large number of bubbles. Nisin itself has the nature of a surfactant, so it is accompanied by foam. After being blown out, collecting and breaking the foam, Nisin was successfully collected, but this method is carried out separately from the fermentation and purification separation process, which does not have a positive impact on the Nisin yield

Method used

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  • Fermentation device and method for efficiently fermenting and producing bacteriocin
  • Fermentation device and method for efficiently fermenting and producing bacteriocin
  • Fermentation device and method for efficiently fermenting and producing bacteriocin

Examples

Experimental program
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Effect test

Embodiment 1

[0075] use figure 1 with figure 2 s installation. Wherein, the fermentation broth circulation device is not used.

[0076] The foam liquid separation device comprises a 2-leaf oblique impeller at the upper end of the stirring shaft, the width of the blade surface is 0.5cm, and the inclination angle is α10°. This embodiment is based on the food and fermentation industry, volume 26 (5): 19-21 literature The reported production of nisin by fermentation of Lactococcus lactis is an example. The culture medium is: water is used as the carrier, and each 1L of culture medium includes the following components by weight:

[0077]

[0078]

[0079] According to the above ratio, 3 L of culture medium was prepared and placed in a 5 L fermenter 1, and the height of the liquid level of the fermented liquid was adjusted to be 1 cm below the bubble liquid separation device.

[0080] Pour the antifoaming agent polyoxypropylene oxide ethylene glyceryl ether with a weight concentration ...

Embodiment 2

[0091] use figure 1 with figure 2 s installation.

[0092] Take Food and Fermentation Industry, Vol. 26(5): 19-21 as an example of the fermentative production of nisin by Lactococcus lactis.

[0093] The difference from Example 1 is that the fermenter is a 50L stainless steel in-situ sterilized fermenter, the oblique impeller of the bubble liquid separation device is 3 leaves, the leaf width is 10cm, the inclination angle is 25°, and the height is adjusted to be above the fermentation liquid surface 3cm.

[0094] With water as the carrier, according to each L containing polyoxyethylene lauryl ether (Brij35) 5g, K 2 HPO 4 10g, sodium acetate 8g, MgSO 4 7H 2 0 2g, ammonium citrate 8g, sucrose 100g, peptone 10g, yeast powder 10g, Tween-80 3mL ratio to prepare 35L of culture medium, put it in a 50L glass fermentation tank, and the height of the bubble liquid separation device is above the surface of the fermentation liquid 3cm.

[0095] And pour 10% volume of antifoaming...

Embodiment 3

[0108] Adopt the method identical with embodiment 1.

[0109] Taking the food and fermentation industry, volume 26 (5): 19-21, Lactococcus lactis fermented to produce nisin as an example, the difference from Example 1 is that the fermenter is a 500L stainless steel in-situ sterilization fermentation Tank, the oblique impeller of the foam liquid separation device is 6 leaves, the width of the blade surface is 15cm, the inclination angle is 65°, the height is adjusted to 5cm above the fermentation liquid surface, other structures are the same as in Example 1, and its operation steps are:

[0110] With water as the carrier, according to each L containing polyoxyethylene monostearate (Myrj52) 3g, K 2 HPO 4 5g, sodium acetate 4g, MgSO 4 7H 2 0 1g, 4g of ammonium citrate, 40g of sucrose, 8g of peptone, 8g of yeast powder, Tween-80 2mL proportioned and prepared medium 350L, installed in a 500L fermenter, the height of the bubble liquid separation device is 5cm above the surface ...

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Abstract

The invention discloses a fermentation device and method for efficiently fermenting and producing bacteriocin. The fermentation device comprises a fermentation tank, a foam-liquid separation device and a foam recycling device, wherein the foam-liquid separation device is arranged in the fermentation tank; an inlet of the foam recycling device is connected with a foam outlet at the top of the fermentation tank; a vacuum hole of the foam recycling device is communicated with a vacuum generation device; a gas inlet is formed in the bottom of the fermentation tank. The method for fermenting and producing the bacteriocin comprises the following steps: filling a culture medium containing a foaming catalyst into the fermentation tank; pouring a de-foaming agent into a collection tank; inoculating bacteria into the fermentation tank; ventilating and adjusting the pH (Potential of Hydrogen) to be 6 to 7, and fermenting; starting the vacuum generation device and collecting foam at the upper part of the fermentation tank into the collection tank; decomposing the foam into liquid and collecting a fermentation product from liquid in the collection tank. By adopting the fermentation device and method disclosed by the invention, the feedback inhibition effect, caused by the product, on bacterium production and metabolism, can be effectively eliminated in time, so that a target product is efficiently expressed.

Description

technical field [0001] The invention relates to a bacteriocin fermentation and purification separation process, in particular to a fermentation separation coupling process for producing bacteriocin. Background technique [0002] Bacteriocins, a class of antibacterial proteins or polypeptides produced by microorganisms, can inhibit Gram-positive bacteria, Gram-negative bacteria and fungi. Bacteriocins have been widely used in aquatic products, food, medicine, biological pesticides and other industries due to their advantages of not polluting the environment, not causing drug resistance of pathogenic bacteria, and enhancing host immunity. For example, nisin (English name Nisin), also known as nisin or nisin, is a small molecule polypeptide. Molecular formula is C 143 h 228 N 42 o 37 S 7 , consisting of 34 amino acid residues, with a molecular weight of about 3500Da. It is considered as an efficient, natural biological food preservative and antibacterial agent. The firs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/21C12M1/02C12P19/04C12R1/01C12R1/125
CPCC12M27/08C12M29/18C12M41/02C12P19/04
Inventor 叶贵子黄青山
Owner 昆山博青生物科技有限公司
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