Composite microbial inoculant capable of efficiently producing methane by grease anaerobic degradation, and preparation method and application thereof

Abstract

The invention provides a composite microbial inoculant capable of efficiently producing methane by grease anaerobic degradation, which comprises the following strains: Anaerovibrio lipolytica DSM 3074, Syntrophomonas erecta DSM 16215, Syntrophomonas bryantii DSM 3014A, Syntrophomonas palmitatica DSM 18709, Desulfovibrio vulgaris DSM 644, Methanospirillum hungatei DSM 13809, Methanobacterium formicicum DSM 1535, Methanobrevibacter smithii DSM861, and Methanosaeta concilii DSM 2139T. The invention also provides a method for preparing the microbial inoculant and application of the microbial inoculant in producing methane by grease anaerobic degradation. The composite microbial inoculant can obviously shorten the start time, enhance the biogas fermentation efficiency and enhance the stability of the fermentation process. The composite microbial inoculant is suitable for different scales of fermentation systems, and has favorable market value.

Description

technical field [0001] The invention relates to the technical field of energy utilization of waste edible oil, in particular to a compound bacterial agent for efficient anaerobic degradation of oil and methane production, as well as a preparation method and application thereof. technical background [0002] Waste edible oils (WEOs) are non-edible animal and vegetable oils produced in the production and operation activities of the catering industry and food processing industry. It is reported that the amount of discarded edible oils and fats in my country accounts for 10% of the total consumption of edible oils. , each year produces . If the untreated waste water containing waste edible oil is directly discharged into the sewer, it will not only cause waste of resources, but also seriously pollute the environment. In particular, due to the rising prices of fossil energy such as petroleum and coal in recent years, the biological conversion of waste oil into new energy has...

AI Technical Summary

Problems solved by technology

Therefore, aiming at the problems of low fermentation inhibition and conversion efficiency in the anaerobic digestion process of oily wastewater and the anaerobic digestion of waste oil as a carbon source, a composite bacterium that efficiently degrades waste edible oil and produces methane has been invented, which can Improve the conversion rate of long-chain fatty acids into volatile acids, thereby increasing the overall efficiency and rate of oil conversion into methane

[0004] At present, there is no compound bacterial agent specifically for the anaerobic degradation of waste edible oil to produce methane. Conventional compound bacterial agents have poor gas production efficiency when using waste edible oil to produce methane.

CN201510037310.5 announced the processing of composite bacteria rich in oil and fat raw materials, but the gas production efficiency of the composite bacteria is not high, and the time required for the cumulative methane production to reach 80% of the final methane production is at least 36 days, and the gas production cycle is too long. economical