Simultaneous saccharification and fermentation hydrogen-producing reactor accompanied with enzyme recycling and experimental method thereof

Abstract

The invention relates to a simultaneous saccharification and fermentation hydrogen-producing reactor accompanied with enzyme recycling. The reactor comprises an enzymolysis unit, a hydrogen-producing unit, an enzyme desorption unit and an enzyme adsorption unit, wherein the enzymolysis unit is located in the hydrogen-producing unit, and is communicated with the hydrogen-producing unit; the lower end of the enzymolysis unit is connected with a feed inlet in the upper end of the enzyme desorption unit; the bottom part of the hydrogen-producing unit is communicated with the top part of the enzyme adsorption unit through a first support pipeline; a discharge hole in the bottom part of the enzyme desorption unit is communicated with the top part of the enzyme adsorption unit through a second support pipeline. The invention also discloses an experimental method of the simultaneous saccharification and fermentation hydrogen-producing reactor accompanied with enzyme recycling. According to the simultaneous saccharification and fermentation hydrogen-producing reactor accompanied with enzyme recycling and the experimental method of the simultaneous saccharification and fermentation hydrogen-producing reactor accompanied with enzyme recycling provided by the invention, cellulase is recycled after a hydrogen-producing process is finished, and the cellulase subjected to enzymatic hydrolysis can be recycled, so that the residual activity of the cellulase is effectively utilized, the sugar yield of unit enzyme is improved, the dosage of the cellulase is further reduced, the process cost can be greatly reduced, and the industrialization production of straw biomass energy conversion is promoted.

Description

technical field [0001] The invention belongs to the technical field of rural energy in agricultural engineering, and in particular relates to a synchronous saccharification and fermentation hydrogen production reactor equipped with enzyme recycling and an experimental method thereof. Background technique [0002] The energy shortage and environmental pollution caused by excessive use of fossil energy make it urgent to produce and use clean energy. Hydrogen energy has a high combustion density, clean and pollution-free, and is considered an attractive alternative energy source. Among various hydrogen production methods, biological hydrogen production using microorganisms to produce renewable energy at normal temperature and pressure has become a research hotspot at home and abroad. [0003] Biological hydrogen production mainly includes hydrogen production by photolysis of water, hydrogen production by photofermentation and hydrogen production by dark fermentation. Among the...

AI Technical Summary

Problems solved by technology

Because if the cellulosic biomass and photosynthetic bacteria are placed in the same reactor, the cellulosic biomass will block the light, which is not conducive to the bacteria receiving light energy, which in turn affects the photosynthesis of photosynthetic bacteria, resulting in low hydrogen production efficiency

[0005] Cellulase plays an indispensable role in the process of biological hydrogen production. It can convert cellulose into fermentable sugar for bacterial fermentation, but due to its high cost, it increases the cost of hydrogen production, which is an obstacle One of the main reasons for the industrial operation of biological hydrogen production

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