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Preparation method and application of a cationic modified glycoprotein microbial flocculant

A microbial flocculant and glycoprotein technology, applied in the field of water treatment, can solve problems such as secondary pollution of the environment, and achieve the effect of simplifying the addition process, simplifying the addition process, and reducing the content

Active Publication Date: 2017-03-15
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Based on the problems existing in the prior art, the present invention provides a method for preparing a cationic modified glycoprotein microbial flocculant, which improves the activity of the glycoprotein microbial flocculant and solves the need for using the glycoprotein microbial flocculant in the prior art Coagulation aids cause secondary pollution of the environment by coagulation aids
[0004] Another object of the present invention is to provide an application of a glycoprotein microbial flocculant modified by the above method to solve the problem that the existing microbial flocculant water treatment process will cause secondary pollution to the environment

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] This embodiment provides a preparation method of a cationic modified glycoprotein microbial flocculant, the method comprising the following steps:

[0032] Step 1, preparing glycoprotein microbial flocculant:

[0033] Step 1.1, prepare seed solution:

[0034] Pick a ring of bacteria from the preservation slant of Klebsiella NY1 strain and insert it into beef extract peptone liquid medium for culture to obtain OD 600nm Klebsiella NY1 seed solution of 1.82 ± 0.08;

[0035] Step 1.2, Klebsiella NY1 fermentation to produce flocculant:

[0036] 100 mL of fermentation culture was sterilized at 121° C. for 30 min. Under a sterile environment, draw 2 mL of Klebsiella NY1 seed solution and add it to the fermentation medium. Under the conditions of 30°C and 160r / min, shake and cultivate at constant temperature for 72 hours to obtain the crude product of glycoprotein microbial flocculant;

[0037] Step 1.3, the purification method of the crude product of glycoprotein microbia...

Embodiment 2

[0064] This embodiment provides a preparation method of a cationic modified glycoprotein microbial flocculant, the method comprising the following steps:

[0065] Step 1, preparing glycoprotein microbial flocculant:

[0066] The specific content of step 1 is basically the same as that of Example 1, except that the stabilizing agent in step 1.3 is potassium chloride.

[0067] Step 2, cationic modification of glycoprotein microbial flocculant:

[0068] Mix 0.02mol of 3-chloro-2-hydroxypropyltrimethylammonium chloride (CTA) with NaOH solution with a concentration of 0.5mol / L at a molar ratio of 1:1.6, let it react for 10min, then add 10g of NY1 Pure flocculant produced by bacteria. The mixture was stirred at room temperature for 0.5 h, put into an incubator and allowed to react at 60° C. for 1 h to obtain a mixture of cation-modified glycoprotein microbial flocculants.

[0069] Step 3, purification of cationized glycoprotein microbial flocculant:

[0070] Completely dissolve ...

Embodiment 3

[0086] This embodiment provides a preparation method of a cationic modified glycoprotein microbial flocculant, the method comprising the following steps:

[0087] Step 1, preparing glycoprotein microbial flocculant:

[0088] The specific content of step 1 is basically the same as that of Example 1, except that the stabilizing agent in step 1.3 is sodium sulfate.

[0089] Step 2, cationic modification of glycoprotein microbial flocculant:

[0090] Mix 0.02mol of 3-chloro-2-hydroxypropyltrimethylammonium chloride (CTA) with 1mol / L NaOH solution at a molar ratio of 1:0.8, let it react for 10min, then add 10g of NY1 bacteria The flocculant produced is pure. The mixture was stirred at room temperature for 2 hours, put into a thermostat to react at 60° C. for 1 hour, and a mixture of cation-modified glycoprotein microbial flocculants was obtained.

[0091] Step 3, purification of cationized glycoprotein microbial flocculant:

[0092] Completely dissolve the mixture obtained in t...

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Abstract

The invention provides a preparation method and application of a cationic modified glycoprotein microbial flocculant. The microbial flocculants are mixed, stirred at room temperature for 0.5 to 2 hours, and then reacted at 60 to 90 ° C for 1 to 3 hours. After purification, a cation-modified glycoprotein microbial flocculant is obtained. The flocculant is used to remove kaolin in wastewater. The cationically modified glycoprotein microbial flocculant prepared by the invention can effectively cationize the negatively charged microbial flocculant, thereby eliminating the coagulation aid during the use of the microbial flocculant. No need to add other flocculants during use, it can remove suspended matter in kaolin simulated wastewater, thereby simplifying the process of adding flocculants. The method of the invention finally simplifies the flocculant addition process, reduces the content of inorganic flocculant substances in the settled sludge, and reduces the secondary pollution of the settled sludge to the environment.

Description

technical field [0001] The invention belongs to the field of water treatment and relates to a microbial flocculant, in particular to a preparation method and application of a cationic modified glycoprotein microbial flocculant. Background technique [0002] Microbial flocculant is a kind of flocculant researched and developed in the late 1980s. It is a kind of metabolites produced by microorganisms with flocculant activity, mainly including glycoprotein, polysaccharide, protein, cellulose and DNA, and bacteria with flocculant activity. body etc. It has been widely studied because of its advantages of high efficiency, non-toxicity, no secondary pollution and wide range of uses. However, the currently produced microbial flocculants are all negatively charged, and the Zeta potential is between -50mV and -80mV. Normally, Ca is required to use 2+ , Mg 2+ Cationic or polyaluminum chloride can be used as a coagulant to improve its activity. When the kaolin in water is 2.5-5g / L...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
Inventor 聂麦茜聂红云
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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