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Lactobacillus plantarum CV10D1 and application thereof

A technology of Lactobacillus plantarum and microorganisms, applied in the field of microorganisms, can solve problems such as the inability to meet the quality requirements of silage, and achieve the effects of improving nutritional value, increasing protein content and improving quality

Active Publication Date: 2019-10-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the traditional silage method in northern Vietnam cannot meet the quality requirements of silage, and it is necessary to develop silage preparations that can effectively inhibit the spoilage fungus of Elephant grass

Method used

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  • Lactobacillus plantarum CV10D1 and application thereof
  • Lactobacillus plantarum CV10D1 and application thereof
  • Lactobacillus plantarum CV10D1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Isolation, culture and physicochemical characteristics of Lactobacillus plantarum CV10D1.

[0049] The Lactobacillus plantarum CV10D1 involved in this example was screened from silage elephant grass samples of farmers in Hanoi, Vietnam.

[0050] (1) Morphological characteristics of Lactobacillus plantarum CV10D1:

[0051] A strain of Lactobacillus plantarum was screened from Viet Nam Silage Elephant Grass by separation and purification, and its strain number was CV10D1. CV10D1 was cultured in MRS medium (according to mass ratio: beef extract 10 g, peptone 10 g, yeast powder 5 g, glucose 20 g, Tween 80 1 mL, K 2 HPO 4 2 g, sodium acetate 5 g, ammonium citrate 2 g, MgSO 4 ﹒ 7H2O 0.58 g, MnSO 4 ﹒ 4H 2 (O 0.25 g, 15 g of agar, add ultrapure water to 1 L, pH6.4-6.6) after culturing on a plate for 48 hours, a colony with a diameter of 0.5-1.5 mm can be formed, the colony is round, the surface of the colony is raised, the edge is neat, moist, The color is milky white ...

Embodiment 2

[0057] Inhibitory effect of live Lactobacillus plantarum CV10D1 on the growth of Fusarium and Aspergillus:

[0058] (1) Inhibitory effect of live Lactobacillus plantarum CV10D1 on the growth of Fusarium and Aspergillus: The double-layer plate confrontation culture method was used to count the number of CV10D1 colonies with a hemocytometer, and the culture solution was diluted to 10 with sterile water. 1 、10 2 、10 3 、10 4 、10 5 CFU / mL, mix 100 µL of the culture solution with 20 ml of MRS medium, invert the plate, and culture at 34 °C for 48 h. Pour into PDA agar medium (potato 200 g, glucose 20 g, agar 20 g), insert 6 mm block of indicator bacteria, culture in a constant temperature incubator at 28 °C for 4 days, and measure the diameter of the bacteria by the cross method. When the number of bacteria in the plate is 10 1 When CFU / mL, Lactobacillus plantarum CV10D1 has a certain inhibitory effect on the two fungi, when the number of bacteria in the plate is 10 2 At the ti...

Embodiment 3

[0077] Application of Lactobacillus plantarum CV10D1 with chitosan in elephant grass silage in northern Vietnam.

[0078] (1) Sample preparation

[0079] Preparation of lactic acid bacteria fermentation stock solution: Lactobacillus plantarum CV10D1 was inoculated into MRS liquid medium (5% inoculum), activated and cultivated at 34 °C for 12 h, and then transferred to fresh MRS liquid medium with 5% inoculum cultured at 34 °C for 24 h, and the fermentation stock solution was diluted or concentrated to a concentration of 10 8 CFU / mL, put in 4 ℃ refrigerator for later use

[0080] Preparation of chitosan solution: Take 1 g of chitosan (deacetylation degree ≥ 90%, molecular weight = 5×10 4 ) was dissolved in 100 mL of 1% citric acid to prepare a 1% chitosan solution.

[0081] Preparation of spoilage molds: The isolated indicator bacteria, such as Penicillium, are cultured on PDA plate medium at 28°C for 2-7 days until spores are formed. Rinse the plate with normal saline, col...

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Abstract

The invention discloses lactobacillus plantarum CV10D1 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 20, 2018, the preservation number is CGMCC No.15466, and the address of the preservation unit is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing. The invention further provides an application of the lactobacillus plantarum CV10D1. The application comprises the restraining effect of the lactobacillus plantarum CV10D1 viable bacteria and fermentation liquor thereof to the growth of aspergillus and fusarium, and the effect of amixture of the lactobacillus plantarum CV10D1 fermentation liquor and chitosan to be used as an additive of pennisetum purpureum silage for jointly restraining the growth of rot fungi in the silage process of pennisetum purpureum, and more technical choices are provided for increasing the quality of the pennisetum purpureum silage through microorganisms.

Description

technical field [0001] The invention relates to plantaractobacillus CV10D1 and its application, in particular to plantaractobacillus CV10D1 and its application in elephant grass silage in northern Vietnam, belonging to the technical field of microorganisms. Background technique [0002] Elephant grass is one of the main livestock feeds in Vietnam. It has high yield, strong adaptability, easy planting, good palatability and high nutritional value. It is an excellent forage grass in Vietnam's animal husbandry, especially the dairy industry. However, there are obvious seasonal differences in the supply of elephant grass. The growth is vigorous in spring and summer, and the yield is high; in autumn and winter, the growth is slow and the yield is low. Therefore, the production of high-quality elephant grass silage can balance the supply fluctuations in forage livestock production throughout the year, which is very important for the stable growth of Vietnam's dairy industry. Elep...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18A23K30/15C12R1/25
CPCA23K30/15A23K30/18C12N1/20C12N1/205C12R2001/25
Inventor 孙群崔雅倩武越河李玺洋余洋周智威
Owner SICHUAN UNIV
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