Lactobacillus plantarum CV10D1 and application thereof

A technology of Lactobacillus plantarum and microorganisms, applied in the field of microorganisms, can solve problems such as the inability to meet the quality requirements of silage, and achieve the effects of improving nutritional value, increasing protein content and improving quality

Active Publication Date: 2019-10-15
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the traditional silage method in northern Vietnam cannot meet the quality requirements of silage, and

Method used

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  • Lactobacillus plantarum CV10D1 and application thereof
  • Lactobacillus plantarum CV10D1 and application thereof
  • Lactobacillus plantarum CV10D1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1:

[0048] Isolation, culture and physical and chemical characteristics of Lactobacillus plantarum CV10D1.

[0049] The Lactobacillus plantarum CV10D1 involved in this example was selected from the silage elephant grass samples of farmers in Hanoi, Vietnam.

[0050] (1) Morphological characteristics of Lactobacillus plantarum CV10D1:

[0051] One strain of Lactobacillus plantarum was screened from Viet Nam silage elephant grass through separation and purification, and its strain was numbered CV10D1. CV10D1 in MRS medium (by mass ratio: 10 g beef extract, 10 g peptone, 5 g yeast powder, 20 g glucose, Tween 80 1mL, K 2 HPO 4 2 g, sodium acetate 5 g, ammonium citrate 2 g, MgSO 4 ﹒ 7H2O 0.58 g, MnSO 4 ﹒ 4H 2 O 0.25 g, agar 15g, add ultrapure water to 1L, pH 6.4 ~ 6.6) After 48 hours of cultivation on the plate, colonies with a diameter of 0.5 ~ 1.5 mm can be formed, the colonies are round, the colony surface is raised, the edges are neat and moist. The color is milky w...

Example Embodiment

[0056] Example 2:

[0057] The inhibitory effect of the live bacteria of Lactobacillus plantarum CV10D1 on the growth of Fusarium and Aspergillus:

[0058] (1) The inhibitory effect of live Lactobacillus plantarum CV10D1 bacteria on the growth of Fusarium and Aspergillus: Adopt double-layer plate confrontation culture method, count the number of CV10D1 colonies with a hemocytometer, and dilute the culture solution to 10 with sterile water. 1 , 10 2 , 10 3 , 10 4 , 10 5 CFU / mL, take 100 µL of the culture medium and mix with 20 ml MRS medium, invert the plate, and incubate at 34 ℃ for 48 h. Pour into PDA agar medium (200 g potato, 20 g glucose, 20 g agar), insert 6 mm indicator bacteria clumps, culture in a 28 ℃ constant temperature incubator for 4 days, and measure the diameter of the bacteria by the cross method. When the number of bacteria in the plate is 10 1 At CFU / mL, Lactobacillus plantarum CV10D1 has a certain inhibitory effect on both fungi. When the number of bacteria in th...

Example Embodiment

[0076] Example 3:

[0077] The application of Lactobacillus plantarum CV10D1 and chitosan in elephant grass silage in northern Vietnam.

[0078] (1) Sample preparation

[0079] Preparation of lactic acid bacteria fermentation stock: inoculate Lactobacillus plantarum CV10D1 into MRS liquid medium (inoculation amount is 5%), activate and cultivate at 34 ℃ for 12 h, and transfer to fresh MRS liquid medium with 5% inoculation amount Incubate at 34 ℃ for 24 h, and dilute or concentrate the fermentation stock to a concentration of 10 8 CFU / mL, placed in a refrigerator at 4 ℃ for later use

[0080] Preparation of chitosan solution: take 1 g of chitosan (degree of deacetylation ≥ 90%, molecular weight = 5×10 4 ) Dissolve in 100 mL 1% citric acid to prepare a 1% concentration chitosan solution.

[0081] Preparation of spoilage mold: The isolated indicator bacteria, such as Penicillium, are cultured on a PDA plate medium at 28°C for 2-7 days until spores are formed. Rinse the plate with physiol...

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Abstract

The invention discloses lactobacillus plantarum CV10D1 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 20, 2018, the preservation number is CGMCC No.15466, and the address of the preservation unit is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing. The invention further provides an application of the lactobacillus plantarum CV10D1. The application comprises the restraining effect of the lactobacillus plantarum CV10D1 viable bacteria and fermentation liquor thereof to the growth of aspergillus and fusarium, and the effect of amixture of the lactobacillus plantarum CV10D1 fermentation liquor and chitosan to be used as an additive of pennisetum purpureum silage for jointly restraining the growth of rot fungi in the silage process of pennisetum purpureum, and more technical choices are provided for increasing the quality of the pennisetum purpureum silage through microorganisms.

Description

technical field [0001] The invention relates to plantaractobacillus CV10D1 and its application, in particular to plantaractobacillus CV10D1 and its application in elephant grass silage in northern Vietnam, belonging to the technical field of microorganisms. Background technique [0002] Elephant grass is one of the main livestock feeds in Vietnam. It has high yield, strong adaptability, easy planting, good palatability and high nutritional value. It is an excellent forage grass in Vietnam's animal husbandry, especially the dairy industry. However, there are obvious seasonal differences in the supply of elephant grass. The growth is vigorous in spring and summer, and the yield is high; in autumn and winter, the growth is slow and the yield is low. Therefore, the production of high-quality elephant grass silage can balance the supply fluctuations in forage livestock production throughout the year, which is very important for the stable growth of Vietnam's dairy industry. Elep...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18A23K30/15C12R1/25
CPCA23K30/15A23K30/18C12N1/20C12N1/205C12R2001/25
Inventor 孙群崔雅倩武越河李玺洋余洋周智威
Owner SICHUAN UNIV
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