Lactobacillus amylovorus for producing feruloyl esterase and application of lactobacillus amylovorus

A technology of Lactobacillus amylovora and ferulic acid esterase, which is applied in the biological field and can solve the problems of low yield of ferulic acid esterase and the inability to meet industrial production.

Active Publication Date: 2015-12-16
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yields of ferulic acid esterase of the reported strains are all low, which cannot meet the requirements of industrial production.

Method used

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  • Lactobacillus amylovorus for producing feruloyl esterase and application of lactobacillus amylovorus
  • Lactobacillus amylovorus for producing feruloyl esterase and application of lactobacillus amylovorus
  • Lactobacillus amylovorus for producing feruloyl esterase and application of lactobacillus amylovorus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Screening and identification of physiological and biochemical characteristics of Lactobacillus amylovorus c strain

[0028] The Shandong silage fermented corn feed sample was washed with sterile water and appropriately diluted, spread on the MRS medium agar plate, cultured at 37°C for 24 hours, picked white round colonies negative for catalase, and the microscopic examination was long Bacillus, repeatedly streaked to determine pure colonies, inoculated into MRS medium, microscopic observation of individual morphology and biochemical reaction measurement, and finally screened to obtain a strain of Lactobacillus amylovorus, which was named Lactobacillus amylovorus (Lactobacillus amylovorus) c.

[0029] The above-mentioned Lactobacillus amylovorus (Lactobacillus amylovorus) c is identified as a Gram-positive bacterium, facultatively anaerobic, non-spore-free, and immobile; the colonies are small and uniform, with neat edges, and milky white; the cells are long ro...

Embodiment 2

[0033] Embodiment 2: Experiment of amylase hydrolyzing starch produced by Lactobacillus amylovorus cCGMCCNo.11056

[0034] Lactobacillus amylovorus (Lactobacillus amylovorus) cCGMCCNo.11056 was transferred to a solid plate containing starch medium, and cultured at 37°C for 48 hours. Observe the clear circles produced by degrading the starch in the medium.

[0035] The formula of the above-mentioned starch-containing medium is: tryptone 10.0g, beef extract 10.0g, yeast powder 5g, soluble starch 10.0g, triammonium citrate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, sodium acetate 3.12g, phosphoric acid Sodium hydrogen disodium 1.63g, potassium acetate 2.25g, Tween-801.0mL, distilled water 1000mL, agar 20.0g for solid, sterilized at 115°C for 30 minutes.

[0036] see results figure 1 .

[0037] Lactobacillus amylovorus (Lactobacillus amylovorus) cCGMCC No. 11056 was transferred to a starch-containing liquid medium, and cultured at 37° C. for 48 hours. Centrifuge a...

Embodiment 3

[0039] Example 3: Ferulic esterase produced by Lactobacillus amylovorus (Lactobacillus amylovorus) cCGMCCNo.11056 hydrolyzing ethyl ferulate

[0040] Lactobacillus amylovorus (Lactobacillus amylovorus) cCGMCCNo.11056 was inoculated into a solid medium containing ethyl ferulic acid, and cultured at 37°C for 72 hours. A clear circle due to the degradation of ethyl ferulate in the medium was observed.

[0041] Above-mentioned formula containing ethyl ferulate medium is: tryptone 10.0g, beef extract 10.0g, yeast powder 5g, DMSO solution 15mL of 10% (W / V) ethyl ferulate, triammonium citrate 2.0g, Magnesium sulfate 0.58g, manganese sulfate 0.25g, sodium acetate 3.12g, disodium hydrogen phosphate 1.63g, potassium acetate 2.25g, Tween-801.0mL, distilled water 1000mL, solids need to add agar 20.0g, sterilize at 115°C for 30 minutes.

[0042] see results figure 2 .

[0043] Transfer Lactobacillus amylovorus (Lactobacillus amylovorus) cCGMCCNo.11056 to a liquid medium containing ethy...

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Abstract

The invention discloses lactobacillus amylovorus for producing feruloyl esterase. Named as (lactobacillus amylovorus)c, the bacterial strain is preserved in China General Microbiological Culture Collection Center on July 6th, 2015 with the preservation number of CGMCC No.11056. Tests show that lactobacillus amylovorus can produce amylase when growing in a starch-containing culture medium and can produce feruloyl esterase when growing in an ethyl-ferulate-containing culture medium, that is, lactobacillus amylovorus has a potential application value in paper-making, textile, food, cosmetics and pharmaceutical industries, particularly, a lactobacillus amylovorus fermented silage can improve the content of lactic acid by 8%, reduce the pH value by 0.27 and shorten the fermentation period by 15 days, and lactobacillus amylovorus has a wide application prospect in preparation of fermented silages and other microecologics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a ferulic esterase-producing Lactobacillus amylovora and application thereof. Background technique [0002] Lactobacillus amylophagous is a lactic acid bacterium with starch hydrolysis activity, and the amylase produced by it has dual activities of amylase and pullulanase, so Lactobacillus amylophagous can directly convert complex starchy substrates to produce lactic acid. At present, the isolation and screening of Lactobacillus amylophagosus that uses starch as carbon source to produce lactic acid from the natural environment has become a hot spot in the field of lactic acid fermentation research. [0003] Ferulic acid esterase is a class of hydrolytic enzymes that can degrade the ester linkages of ferulic acid and lignin or arabinosemiose in plant cell walls. Ferulic acid esterase has a synergistic effect with other cellulase such as xylanase and arabinosidase, and can improve the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/00C12R1/225
Inventor 孔健徐振上
Owner SHANDONG UNIV
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