19 results about "Cell wall degradation" patented technology

The invention relates to application of tryptophol in prevention and treatment of corn bacterial stem rot and relates to the technical field of biological prevention and control, the pathogenic bacterium of the corn bacterial stem rot is Dickeya zeae, it is found for the first time that tryptophol can inhibit the mobility of the Dickeya zeae, reduce the activity of cell wall degrading enzyme and relieve the disease degree, and the tryptophol can inhibit the activity of the Dickeya zeae, so that the tryptophol can be used for preventing and treating the corn bacterial stem rot. The tryptophol can effectively reduce the pathogenicity of tryptophol, but has no adverse effect on the cell viability and form of the corn dibasic bacteria, which indicates that the effect of tryptophol on the corn dibasic bacteria is realized by inhibiting the pathogenicity of bacteria instead of killing bacteria, so that tryptophol acts on the corn bacterial stem rot, the drug resistance is not easy to generate, and the tryptophol can be used for treating the corn bacterial stem rot. The tryptophol is expected to become a substitute of antibiotics and is used for preventing and treating the corn bacterial stem rot, the diseases can be effectively controlled, the selective pressure of drug resistance development can be relieved, and a new solution is provided for sustainable prevention and treatment of the corn bacterial stem rot.

This RNA extraction method comprises: (1) adding an alkali metal salt and a cell wall-degrading enzyme to a sample to prepare a reaction solution containing the alkali metal salt and the cell wall-degrading enzyme, the final concentration of the alkali metal salt being 50 mM to 150 mM; (2-1) incubating the reaction solution to degrade the cell walls of microorganisms contained in the reaction solution; and (3) extracting RNA from the reaction solution after the incubation. This reagent set for RNA extraction contains: an alkali metal salt solution in which the concentration of the alkali metal salt is 50 mM or more; and a cell wall degrading enzyme.

The invention belongs to the technical field of genes, and particularly relates to a marker for identifying storage and preservation effects of strawberries as well as a screening method and application of the marker. The expression quantity of various cell wall degradation related genes in strawberry fruits treated by a storage method is measured, fruit preservation physiological indexes are combined, and comprehensive sorting is performed by homogenizing data through an assignment method; and analyzing the correlation between gene expression and assignment scores of fruit preservation physiological indexes in different treatments, and screening out genes with remarkable correlation as strawberry preservation markers. The marker disclosed by the invention can be used for quickly and accurately evaluating and predicting the quality and the fresh-keeping effect of the picked strawberries.

The invention discloses a fresh-keeping storage method of plum fruits, which is characterized in that a compact network structure of a shellac-pullulan-gellan gum basic composition effectively obstructs invasion of external microorganisms, and significantly slows down loss of water in the fruits, so that the plump appearance and hardness of the fruits are maintained, and the fruit quality is improved. The siRNA and the AVG wrapped by the lipidosome accurately interfere with the ripening and senescence process in the fruit from the molecular level, the siRNA specifically silences the gene expression of cell wall degradation key enzyme and delays the softening process of the fruit, and the AVG effectively blocks the biosynthesis pathway of ethylene and inhibits the start of an after-ripening signal, so that the ripening and senescence process of the fruit is delayed. The external formed physical film provides a stable microenvironment for internal bioactive molecules, protects the internal bioactive molecules from being degraded and prolongs the action time, and the internal molecular regulation delays the disintegration of cell structures, so that the external film can be attached more enduringly and exerts a barrier function, and the maintenance of the fruit quality is realized under a refrigeration condition.

The invention provides a dogwood health wine soaking process based on model prediction optimization, and relates to the technical field of dogwood health wine soaking processes, and the dogwood health wine soaking process comprises the following steps: calculating a comprehensive cell wall degradation index, grading raw materials, and coding the graded raw materials into raw material quality grade values; establishing a first-order dissolution kinetic model, and performing parameter optimization in combination with a golden section search algorithm to obtain an optimal infusion temperature and an optimal infusion time; weighing the medicinal materials according to a preset ratio, mixing the medicinal materials with the base liquor, and starting to soak; arranging a two-parameter collaborative monitoring system in the soaking container; constructing a random forest model, predicting the dissolution completion degree and the predicted residual equilibrium time, performing dynamic regulation and control, and ending the dynamic regulation and control process when the prediction result meets the requirement to obtain an immersion liquid; and taking out the soaking liquid from the soaking container, and separating and purifying by adopting a secondary filtering process to obtain the dogwood health wine product. The technical defects that a traditional process depends on experience, parameters are solidified, and the monitoring means is single can be overcome.

The present invention relates to a series of cell wall degrading enzymes with antibacterial activity for effectively controlling Staphylococcus aureus, and the cell wall degrading enzymes with antibacterial activity of the present invention are a series of lyases with new amino acid sequences. The cell wall degrading enzymes with antibacterial activity of the present invention can be used as agents capable of effectively inhibiting Staphylococcus aureus, and can be applied to the disinfection of Staphylococcus aureus in the environment and the treatment of diseases caused by Staphylococcus aureus infection, etc.

Due to the constituent components of the cell walls of filamentous fungi, the texture when eating a filamentous fungus body resembles a paper-like texture, and applications other than meat analogs are limited. Additionally, filamentous fungus bodies have characteristics of absorbing water but also readily releasing water, and having no binding property between mycelia, and thus even when a crushing treatment is performed, it has been difficult to process the result into foodstuffs that have a feeling of unity due only to a filamentous fungus culture product, or into foodstuffs that have uniform and smooth paste-like physical properties. This edible filamentous fungus body having an adjusted texture can be provided by treating a filamentous fungus body with a cell wall degrading enzyme.

The invention discloses a high-temperature treatment method for promoting coloring of bagged peaches after picking and keeping hardness, and belongs to the technical field of treatment of fruits after picking. The method aims to solve the problems of post-harvest color difference caused by omission of a bag picking link and easy softening caused by accelerated synthesis of ethylene in the existing bagged peaches, and avoids the risk of chemical reagent residues. According to the technical scheme, the method comprises the following steps: pre-treating bagged peach fruits which are 70-80% in maturity after harvesting and are free of damage, continuously treating the bagged peach fruits in a constant-temperature environment with the temperature of 30-35 DEG C and the relative humidity of 85-90% for 6-9 days, and finally transferring the bagged peach fruits into a low-temperature warehouse with the temperature of 0-2 DEG C for storage for 7-8 days. The core principle is that ethylene synthesis key enzyme activity is inhibited through specific high temperature, ethylene generation is reduced, accumulation of pigments such as anthocyanin is promoted to improve coloring, cell wall degradation is slowed down to maintain hardness, and quality stability is further maintained through subsequent low-temperature storage. The method adopts pure physical temperature regulation and control, is simple and convenient to operate, low in cost and suitable for large-scale popularization, can synchronously improve the appearance color and texture quality of the bagged peaches after being picked, prolongs the storage period and shelf life, and remarkably improves the commodity value and market competitiveness of the fruits.

The invention relates to an application of a plant secondary metabolite in inhibiting pathogenicity of fusarium oxysporum. The application comprises the step of realizing an antibacterial effect through at least one mechanism of inhibiting hypha growth, spore germination, cell membrane permeability change, defensive enzyme activity reduction, cell wall degrading enzyme activity inhibition or toxin synthesis reduction of pathogenic bacteria. The antibacterial action mode of the 2, 4-di-tert-butylphenol is that the 2, 4-di-tert-butylphenol can destroy the completeness of cell membranes of fusarium oxysporum of cucumbers and disturb the functions of the cell membranes to cause leakage of cell contents and oxidative damage, so that normal physiological activities of cells are inhibited. Meanwhile, 2, 4-di-tert-butylphenol can inhibit defensive enzyme activity and cell wall degrading enzyme activity of fusarium oxysporum and has a remarkable inhibition effect on toxin synthesis, so that the purpose of inhibiting fusarium oxysporum hyphae from infecting host plants is achieved, and a basis is provided for subsequent deeper research on a fusarium oxysporum inhibition mechanism.

The present invention relates to a composition comprising at least one antifungal compound with cell membrane interacting properties and at least one cell wall degrading enzyme, optionally wherein the at least one antifungal compound with cell membrane interacting properties is a polypeptide, optionally an antibody or fragment thereof such as a heavy chain variable domain of a heavy chain antibody (VHH) or a functional fragment thereof, which antibody or fragment thereof is capable of binding to a fungus. The invention relates to cell wall degrading enzymes, more specifically cell wall degrading enzymes according to SEQ ID NOs: 100 to 130 or mixtures thereof, and compositions or combinations thereof further comprising at least one antifungal compound with cell membrane interacting properties. The invention further relates to methods of producing, methods of using and the use of the compositions and combinations disclosed herein. The application further relates to transgenic plants, or methods for producing the same, comprising one or more of a polynucleotide according to the invention.

This invention relates to a preparation process for tomato sauce, belonging to the field of food processing technology. Addressing the problems of low cell wall degradation efficiency and severe damage to heat-sensitive components in existing technologies, this invention provides a preparation process: adjusting the pH of tomato pulp to 4.40-4.55; adding pectinase followed by cellulase at 40-50℃ for stepwise enzymatic hydrolysis; rapidly heating to 80-90℃ at a rate of 4.8-5.2℃ / min and maintaining this temperature for 100-120 seconds to inactivate the enzymes; adjusting the pH to 4.25-4.35; vacuum concentrating until the soluble solids content reaches 25-30%; and then homogenizing, filling, and sterilizing to obtain the finished product. This invention, through the synergistic effect of stepwise enzymatic hydrolysis and precise enzyme inactivation, significantly improves cell wall degradation efficiency and the retention rate of heat-sensitive components, while inhibiting the generation of cooked flavor, making it suitable for the industrial production of tomato sauce.

The invention discloses application of a banana MaSCL8 gene in regulation and control of fruit ripening. The nucleotide sequence of the banana MaSCL8 gene is as shown in SEQ ID NO. 1. According to the invention, a MaSCL8 transcription factor is identified from banana fruits, and the gene is dominantly expressed in the fruits. Through overexpression of MaSCL8 in tomatoes and observation of tomato fruits, it is found that compared with a wild type, the tomato fruits of the overexpressed MaSCL8 strain are mature ahead of time, the color value and hardness of the fruits are rapidly reduced, and the ethylene yield has a peak value ahead of time. Meanwhile, the expression levels of starch degradation genes SlAMY2 and SlBAM1 and a cell wall modification gene SlEXP1 in fruits of the overexpressed MaSCL8 strain are obviously improved compared with those of wild fruits. The research results reveal that the MaSCL8 promotes fruit ripening by activating starch and cell wall degradation genes, the action mechanism of the MaSCL8 participating in regulation and control of fruit ripening is defined, and the gene can be used as a candidate gene for improving banana fruit quality.

The invention relates to the technical field of feed science and biology, and discloses a Shiqi young pigeon functional feed containing herbal tea residues and a preparation method of the Shiqi young pigeon functional feed, and the Shiqi young pigeon functional feed takes a biological modified herbal tea residue fiber carrier, anchored plant active matter and metabiotics, dormant state functional remodeling probiotics and galactooligosaccharide as main components. The preparation method comprises the following steps: carrying out synergistic solid-state fermentation on the herbal tea residue matrix by adopting lactobacillus plantarum and bacillus subtilis; adding a compound enzyme preparation into the material to perform targeted enzymolysis activation in a specific chemical microenvironment at a fermentation end point; and carrying out probiotic function remodeling treatment and low-temperature stabilization on the materials subjected to enzymolysis. Through multi-stage biological coupling treatment, cell walls of the herbal tea residues are effectively decomposed, antinutritional factors are degraded, and functional components such as probiotics, metabiotics and plant active matters are organically integrated into the same carrier. The product provided by the invention can significantly improve the growth performance, feed utilization rate and immune index of young pigeons.

The invention belongs to the technical field of traditional Chinese medicine processing, and particularly relates to an extraction method for directionally improving the content of antioxidant and anti-inflammatory active ingredients of traditional Chinese medicine and the antioxidant and anti-inflammatory active ingredients. The method comprises the following steps: grinding traditional Chinese medicinal materials into powder in a grinding machine, adding the powder into an MRS bouillon liquid culture medium, inoculating lactobacillus plantarum after high-pressure sterilization, standing for fermentation, and centrifuging to take supernate, so as to obtain the traditional Chinese medicinal fermentation liquor. According to the method, a single lactobacillus plantarum scheme is adopted to focus on efficient conversion of flavonoid components, single advantages of beta-glucosidase and cell wall degrading enzyme are relied on, combined flavonoids can be converted into free flavonoids through short-time fermentation, the content of total sugar, flavonoids and polyphenols in the medicinal materials is remarkably increased, the oxidation resistance is remarkably improved, meanwhile, the process is simple and short, consumed time is short, and the method is suitable for industrial production. The method is suitable for large-scale production, free of organic solvent residues, green and safe.

The invention discloses an application of a coumarin small molecule compound derived from Zanthoxylum avicennae in inhibition of activity of beta-glucosidase. According to the invention, it is determined for the first time that Zanthoxylum avicennae sourced coumarin small molecule compound Rubigoligolactone can inhibit the activity of beta-glucosidase by directly binding Y193, D571 and E575 sites. The beta-glucosidase inhibitory activity of the rugoligolactone is utilized, so that cell wall degradation can be effectively prevented, field fusarium fungus prevention and control can be realized, and the application potential is huge.

The invention provides a microalgae lipid extraction method using triple-effect cell wall degrading enzyme synergy and ultrasonic assistance, which comprises the following steps: suspending microalgae powder in a buffer solution containing triple-effect cell wall degrading enzyme, and carrying out enzymolysis to obtain a microalgae enzymolysis mixed suspension; the triple-effect cell wall degrading enzyme comprises cellulase, pectinase and mannase; carrying out ultrasonic treatment on the microalgae enzymolysis mixed suspension in an ice bath, centrifuging, discarding supernatant, and collecting precipitated biomass to obtain wall-broken algae powder; the ultrasonic treatment adopts a pulse mode; mixing the wall-broken algae powder with a mixed solvent of n-hexane and isopropanol, extracting, centrifuging to take supernate, and evaporating and concentrating to obtain the lipid extract. A triple-effect cell wall degrading enzyme system is adopted to precisely degrade a polysaccharide network of microalgae cell walls through a synergistic effect; secondly, ultrasonic treatment is carried out under the ice-bath low-temperature condition, the algal alkene hydrophobic barrier on the outer layer of the microalgae cell wall can be efficiently and selectively damaged, and finally the lipid extraction rate is increased.

This invention provides an AP2 transcription factor that promotes hemicellulose degradation. DkAP2 The DkAP2 The nucleotide sequence is shown in SEQ ID NO.1. This invention also provides the above-mentioned AP2 transcription factor. DkAP2 Application in promoting hemicellulose degradation. This invention is the first to isolate and identify the AP2 transcription factor from persimmon that can positively promote hemicellulose degradation. DkAP2 Overexpression of this gene in persimmon fruit can promote the degradation of hemicellulose in the cell wall, achieving precise regulation of the softening process of persimmon fruit. Overexpression of this gene in tobacco leaves can also induce cell wall degradation and soften the leaves, proving that its function is conserved across species. It can also be further applied to plants from lignocellulose biomass sources to effectively improve the degradation efficiency of hemicellulose, providing a new, efficient, and environmentally friendly molecular regulation method for biofuel production, livestock feed processing, and the paper industry.

This invention belongs to the fields of plant molecular biotechnology and genetic engineering, specifically relating to the application of an expression promoter of transcription factor DkAS1 in regulating the ripening and softening of persimmon fruit. The amino acid sequence of DkAS1 is shown in SEQ ID NO.1. This invention utilizes biotechnology to isolate the gene encoding the transcription factor DkAS1 in persimmon fruit and discovers... DkAS1 It is induced by gibberellin treatment and can inhibit cell wall degradation, ethylene and abscisic acid synthesis, and participate in the regulation of the postharvest softening process of persimmon fruit.

This invention discloses a method for preparing controllable degradable archaeological-style wood, comprising the following steps: Step 1, pre-treatment of the wood substrate by low-temperature plasma etching activation; Step 2, impregnation with a composite enzyme degradation solution using a vacuum pressure method; Step 3, segmented temperature-pressure synergistic directional degradation; Step 4, injection of inorganic nanoparticles into the degraded wood for structural fine-tuning; Step 5, impregnation of the wood with a temperature-sensitive polymer solution, utilizing the phase transition principle to block cell wall degradation sites; Step 6, gradient vacuum drying and surface aging treatment. This invention solves the problems of uneven degradation, process loss of control, and low simulation degree of traditional methods. The prepared archaeological-style wood exhibits stable properties, uniform degradation, and a microstructure that highly replicates real cultural relics.