Method for extracting and separating cyclocarya paliurus flavonoids

A technology of Cyclocarya flavonoids and Cyclocarya flavonoids, applied to medical preparations containing active ingredients, pharmaceutical formulas, plant/algae/fungus/moss components, etc., can solve the problem of destroying the active ingredients of Cyclocarya paliurus, unfavorable large-scale industrialization, Complex extraction methods and other issues, to achieve the effects of beneficial development and utilization, easy industrial production, and low cost

Inactive Publication Date: 2018-11-30
WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction methods of flavonoids in Cyclocarya paliurus are complicated and costly, which is not conducive to large-scale industrialization
In addition, the traditional raw material pretreatment methods are mostly high-temperature drying. At the same time, the traditional water method also needs to use high-temperature heating to extract a large amount of extracts, which will not only destroy the active ingredients in Cyclocarya paliurus, but also increase the process. energy consumption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for extracting polysaccharide from Radix pennywort provided by the present embodiment comprises the following steps:

[0019] a. Wash the prickly ash with water, dry it at 50°C, pulverize the sample with a pulverizer, and pass it through a 40-mesh sieve.

[0020] b. Acid hydrolysis: Add sagebrush to distilled water to make pulp, the weight ratio of sagebrush to distilled water is 1:2, add food-grade hydrochloric acid (hydrochloric acid ≥ 31.0%), and the weight of food-grade hydrochloric acid added is 10% of the weight of the slurry , 40 ℃ maintained for 2h.

[0021] c. Microbial fermentation and degradation: add the acid-hydrolyzed raw materials and heat up to 40°C, adjust the pH to 6, add 0.3 parts of yeast and Bacillus subtilis strains, mix well, and ferment and degrade for 1 day.

[0022] d. Compound enzymolysis: add compound enzyme to the material to be extracted after fermentation, the weight ratio of compound enzyme and material is 1:40, adjust pH.0, kee...

Embodiment 2

[0028] A method for extracting polysaccharide from Radix pennywort provided by the present embodiment comprises the following steps:

[0029] a. Wash the prickly ash with water, dry it at 40°C, pulverize the sample with a pulverizer, and pass it through a 40-mesh sieve.

[0030] b. Acid hydrolysis: Add sagebrush to distilled water to make pulp, the weight ratio of sagebrush and distilled water is 1:1, add food-grade hydrochloric acid (hydrochloric acid ≥ 31.0%), and the weight of food-grade hydrochloric acid added is 5% of the weight of the slurry , 30 ℃ maintained for 1h.

[0031] c. Microbial fermentation and degradation: add the acid-hydrolyzed raw materials and heat up to 37°C, adjust the pH to 5, add 0.05 part of yeast and Bacillus subtilis strains, mix well, and ferment and degrade for 0.5 days.

[0032] d. Compound enzymolysis: add compound enzyme to the material to be extracted after fermentation, the weight ratio of compound enzyme and material is 1:20, pH is adjuste...

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PUM

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Abstract

The invention discloses a method for extracting and separating cyclocarya paliurus flavonoids, wherein the method comprises the following steps: a, washing cyclocarya paliurus with clear water, dryingat the temperature of 40-50 DEG C, crushing the sample by a pulverizer and allowing to pass through a 40 mesh sieve; b, carrying out acidolysis; c, carrying out microbial fermentation degradation; d,carrying out compound enzymatic hydrolysis; e, carrying out acetone extraction; f, carrying out purification of crude flavonoids: purifying flavonoids with activated carbon. In the extraction processof the cyclocarya paliurus flavonoids, firstly, cell walls of cyclocarya paliurus are degraded by the preliminary microbial fermentation method, and some impurities, such as polysaccharides, are removed by microbial action; then the flavonoids are fully released by enzymatic hydrolysis. The method has no pollutant discharge in the whole technological process, is suitable for large-scale production, is beneficial to the development and utilization of cyclocarya paliurus resources, has the advantages of simple steps, low cost, strong operability and high output, and is prone to industrialized production.

Description

technical field [0001] The invention relates to a method for extracting and separating the flavonoids of pennywort. It belongs to the field of marine microalgae cultivation. Background technique [0002] Green money willow, (Latin name: Cyclocaryapaliurus.) Aliases: cash cow, hemp willow, green money plum, mountain hemp willow, mountain tree. Juglandaceae and Chrysanthemum genus are found in many provinces in southern my country, and they are mostly distributed sporadically. Qingqianliu is a rare tree species that survived the quaternary period of the glacier, and it only exists in China. Qingqianliu is known as the giant panda in the plant world and the third tree in the medical world. It is known that the first tree in the medical field, the willow tree, produced aspirin, which is anti-inflammatory, bactericidal, and anti-thrombotic, opening the first change in the history of human health. [0003] Modern research has confirmed that the leaves of Qingqian willow have t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61P3/10A61P9/12A61P3/06A61P37/04A61P39/06
CPCA61K36/52A61K2236/19A61K2236/331A61K2236/55A61P3/06A61P3/10A61P9/12A61P37/04A61P39/06
Inventor 郑晓杰周环胡霞何悦章志诚
Owner WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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