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Soil treatment agent for preventing and treating root-knot nematode disease and preparation method and application method thereof

A root-knot nematode disease and soil treatment technology, applied in the direction of nematicides, botany equipment and methods, applications, etc., can solve the problem of large-scale promotion and application, and there is still a long distance to limit the efficacy and control effect of microbial agents. No obvious problems, to ensure safe and sustainable growth, reduce the incidence of root-knot nematode diseases, and reduce the hatching rate

Inactive Publication Date: 2018-10-16
NANJING NORMAL UNIV CHANGZHOU INST OF INNOVATION & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely complex soil environment and the ubiquity of soil antibacterial effects, the effectiveness of microbial agents is largely limited. Moreover, for soils with a high number of root-knot nematodes, the control effect of directly adding microbial agents not significant
In addition, methods such as screening root-knot nematode-resistant varieties and introducing nematode-resistant genes through genetic engineering have broad application prospects, but most of them are currently in the laboratory research stage, and there is still a long way to go before large-scale application

Method used

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  • Soil treatment agent for preventing and treating root-knot nematode disease and preparation method and application method thereof
  • Soil treatment agent for preventing and treating root-knot nematode disease and preparation method and application method thereof
  • Soil treatment agent for preventing and treating root-knot nematode disease and preparation method and application method thereof

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Experimental program
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preparation example Construction

[0044] 2. Preparation of thread-killing main agent and thread-killing synergist

[0045] 2.1 Preparation of main agent for killing nematodes containing bagasse

[0046] 1) The bagasse is thoroughly pulverized as an organic raw material for killing threads. The technical indicators of the organic raw material are: moisture: 10%; total organic carbon: 40%; total organic nitrogen: 0.5%; easily oxidizable carbon: 8%;

[0047] 2) Bacillus and Aspergillus are mixed in a mass ratio of 50:50 as an auxiliary material for promoting decomposition, wherein the Bacillus is Bacillus amyloliquefaciens;

[0048] 3) In terms of mass percentage, 1% of the above-mentioned decomposition-promoting auxiliary materials, and the balance is line-killing organic raw materials;

[0049] The above-mentioned nematode-killing organic raw materials and decomposition-promoting auxiliary materials were mixed at a speed of 100 r / min, dried at 70° C. for 10 minutes, and then naturally cooled at room temperatur...

Embodiment 1

[0075] Use the soil treatment agents obtained in 2.1, 2.3 and 2.4 to carry out the nematode test in the soil with high incidence of eustoma nematode disease in continuous cropping for six years.

[0076] 1) Soil sample collection

[0077] The soil samples were collected from Greenhouse No. 10 of Eustoma planting base of Shiping Liran Flowers and Fruits and Vegetables Co., Ltd., Honghe Prefecture, Yunnan Province. Eustoma has been planted in this greenhouse for six years and the root-knot nematode disease is serious, with an incidence rate of about 50%. The collected soil samples were first watered to 50-60% of the maximum water holding capacity in the field, cultured at 25°C for 5 days, and then dried for 3 days to be used to promote the hatching of nematode eggs.

[0078] 2) Experimental design

[0079] Three experiments were set up in the indoor cultivation experiment:

[0080] 1) Control (CK) without any treatment;

[0081] 2) Soil treatment agent treatment (SB) containi...

Embodiment 2

[0092] The bagasse and alfalfa powder soil treatment agents obtained in 2.1, 2.2 and 2.4 were used to conduct a nematode test in the soil with high incidence of eustoma nematode disease in continuous cropping for six years.

[0093] 1) Soil sample collection

[0094] The soil samples were collected from Greenhouse No. 17 of Eustoma planting base of Shiping Liran Flower Fruit and Vegetable Co., Ltd., Honghe Prefecture, Yunnan Province. Eustoma has been planted in this greenhouse for six years and the root-knot nematode disease is extremely serious, with an incidence rate as high as 85%. The collected soil samples were first watered to 50-60% of the maximum water holding capacity in the field, cultured at 25°C for 5 days, and then dried for 3 days to be used to promote the hatching of nematode eggs.

[0095] 2) Experimental design

[0096] Three experiments were set up in the indoor cultivation experiment:

[0097] 1) Control (CK) without any treatment;

[0098] 2) Bagasse soil...

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Abstract

The invention discloses a soil treatment agent for preventing and treating the root-knot nematode disease and a preparation method and an application method thereof. The soil treatment agent is prepared from a nematode killing main agent and a nematode killing synergist; the nematode killing main agent is prepared from a nematode killing organic raw material and a decomposition promoting accessory; when the nematode killing organic raw material is a solid organic raw material, the nematode killing organic raw material is prepared from at least one of crop straws and sugar refinery offcut; whenthe nematode killing organic raw material is a liquid organic raw material, the nematode killing organic raw material is prepared from at least one of alcohol fermentation liquor and molasses; the decomposition promoting accessory is prepared from at least one of organic catalytic material and a special microorganism. The soil treatment agent disclosed by the invention can secrete to generate a great amount of nematode killing substances, such as an cell wall degrading enzyme, acetic acid and the like, in the indigenous microorganism anaerobic decomposition process, and a rapidly created anaerobic environment can also kill nematodes, so that the number of nematodes in soil with a high root-knot nematode disease incidence rate and a proportion of the category in a nematode community are reduced, and a root-knot nematode disease incidence rate of succeeding crops is reduced.

Description

technical field [0001] The invention relates to a soil treatment agent for preventing and controlling root-knot nematodes, a preparation method and an application method thereof, and belongs to the field of pest control in facility agriculture. Background technique [0002] Root-knot nematodes are a very important class of plant pathogenic nematodes, widely distributed all over the world. Because root-knot nematodes have many effective species and a wide range of hosts, they can infect almost all common crops, such as vegetables, flowers, tobacco, fruit trees and weeds, seriously threatening the sustainable production of agriculture. In recent years, with the expansion of vegetable planting area in protected areas and the extension of continuous cropping years, especially the large-scale promotion of solar greenhouses, crops have been severely cropped in four seasons, resulting in the continuous expansion of the occurrence area of ​​root-knot nematode disease, and the damage...

Claims

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Application Information

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IPC IPC(8): A01N65/44A01N63/04A01N63/00A01N43/16A01N37/02A01P5/00A01G13/00A01G13/02A01B79/02
CPCA01B79/02A01G13/00A01G13/0275A01N37/02A01N43/16A01N63/00A01N63/30A01N65/00A01N65/20A01N65/44
Inventor 赵军朱佳双常亚锋黄新琦张金波蔡祖聪
Owner NANJING NORMAL UNIV CHANGZHOU INST OF INNOVATION & DEV
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