24 results about "Cellulose binding" patented technology

A semiconductor material and a multilayer semiconductor material are earth-conscious and are less harmful to living organisms. The semiconductor material has fibers containing, as a main component, a filament derived from at least any one of a wood material, a plant fiber (pulp), an animal, an alga, a microorganism and a product produced by a microorganism, and has N-type negative resistance. It is preferred that the fibers include bundles of cellulose nanofibers (CNFs), and the width of each of the bundles be 30 to 50 nm. It is also preferred that the fibers are fibers in which a plurality of hydroxy groups and a plurality of carbonyl groups be bound to cellulose.

The invention provides a preparation method and application of a CBD-AL fusion recombinant protein, a variant Z region obtained by site-directed mutagenesis of a B region of SPA is connected in series with a B structural domain of a protein L, codon optimization is carried out, the front end of CBD is introduced into a BamHI restriction enzyme cutting site, a cysteine sequence, a termination codon TAA and an EcoRI restriction enzyme cutting site are added to the tail end of the B region of the recombinant protein L, and the CBD-AL fusion recombinant protein is obtained. BamHI and EcoRI are subjected to double enzyme digestion and then are connected to a PET28a vector subjected to double enzyme digestion, a recombinant protein CBD-AL gene expression vector of a structural domain of strongest binding IgG of protein A and protein L connected with CBD is constructed, the recombinant protein CBD-AL gene expression vector is transferred into an expression strain for amplification culture, an inducer is added for induction, bacterial sludge is collected, purification is performed by cellulose affinity chromatography, and the recombinant protein CBD-AL gene expression vector is obtained. A binding protein which has a cellulose binding function and can bind all types of immunoglobulins and subtypes thereof is prepared.

The invention relates to the fields of hydrogel 3D printing, flexible sensors and wearable electronic equipment, in particular to cellulose-based hydrogel meeting 3D printing and sensing performance as well as a preparation method and application of the cellulose-based hydrogel. The cellulose-based hydrogel contains zinc salt, lithium salt, water and cellulose subjected to alkalization pretreatment, in the cellulose-based hydrogel, the total mass of zinc salt, lithium salt and water is 100%, the content of the cellulose subjected to alkalization pretreatment is 1%-6%, the molar ratio of the total amount of the zinc salt and the lithium salt to the water is 1: (3-4), the molar ratio of the zinc salt to the lithium salt is 1: (0.4-2.4), and the molar weight of the zinc salt and the molar weight of the lithium salt are both based on the content of the zinc element and the content of the lithium element. According to the cellulose-based hydrogel and the preparation method thereof, the cellulose subjected to alkalization pretreatment is adopted, the ratio of the cellulose subjected to alkalization pretreatment and the metal salt solution (containing the zinc salt, the lithium salt and the water) with the specific content and the composition of the metal salt solution are combined, the cellulose-based hydrogel meeting the sensing and 3D printing performance is obtained, and the cellulose-based hydrogel has the better anti-freezing performance; the composite material has relatively high conductivity and relatively good mechanical properties, and an unexpected technical effect is achieved.

The invention discloses a method for preparing a carbon nanomaterial / cellulose composite material in a'bridge 'mode, and belongs to the field of carbon nanomaterial / cellulose composite materials. According to the invention, a polypeptide nanofiber'bridge 'material is synthesized; cellulose surface modification; magnetically stirring, mixing and reacting the synthesized amphiphilic polypeptide nanofiber'bridge 'material, surface-modified cellulose and a carbon nanomaterial at room temperature, and drying a precipitate obtained by centrifugation at room temperature to prepare the carbon nanomaterial / cellulose composite material. According to the method for preparing the carbon nanomaterial / cellulose composite material in a'bridge 'mode, amphiphilic polypeptide molecules or polypeptide nanofibers are introduced to serve as a'bridge' for combining the carbon nanomaterial and cellulose, firm connection is formed between the carbon nanomaterial and the cellulose, combination of the carbon nanomaterial and the cellulose is effectively promoted, the method is simple and easy to implement, and the method is suitable for industrial production. And more excellent stability and mechanical properties can be obtained.

The invention relates to a modified cellulase derived polypeptide and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The invention provides a derivative polypeptide of cellulose excision enzyme Cel7A, the cellulose excision enzyme Cel7A is derived from Trichoderma reesei, NCBI Acession No.XM006969162.1, and the amino acid sequence of the cellulose excision enzyme Cel7A is as shown in SEQ ID NO: 1. The invention further provides a preparation method of the derivative polypeptide of the cellulose excision enzyme Cel7A. Wherein the amino acid sequence of the cellulose binding domain CBM is as shown in SEQ ID NO: 2. The derivative polypeptide is a polypeptide of which the amino acid sequence is 70% or more identical to that of a polypeptide shown in SEQ ID NO: 2, can reduce combination with lignin and improve the degradation efficiency of cellulose, so that the saccharification efficiency of cellulase is improved, the post-treatment process cost is greatly saved, and the derivative polypeptide is suitable for industrial production. The method has important industrial application value.

The present application relates to the field of hydrogel 3D printing, flexible sensor, wearable electronic device, in particular to a cellulose-based hydrogel meeting 3D printing and sensing performance, and a preparation method and application thereof.The cellulose-based hydrogel contains zinc salt, lithium salt, water and alkali pretreated cellulose;in the cellulose-based hydrogel, the content of the alkali pretreated cellulose is 1%-6% based on 100% of the total mass of the zinc salt, lithium salt and water, the molar ratio of the total amount of zinc salt and lithium salt to water is 1:(3-4), the molar ratio of zinc salt to lithium salt is 1:(0.4-2.4), and the molar amount of zinc salt and lithium salt is calculated based on the content of zinc element and lithium element.The present application uses alkali pretreated cellulose, combines the ratio of alkali pretreated cellulose and metal salt solution (containing the above-mentioned zinc salt, lithium salt and water) with a specific content, and the composition of the metal salt solution, to obtain a cellulose-based hydrogel meeting sensing and 3D printing performance, and having better anti-freezing performance, higher conductivity and better mechanical performance, achieving unexpected technical effects.

The present invention relates to a process wherein one or more dye precursors, e.g., indole, are provided to a textile and converted by one or more enzymes, e.g., an oxidizing enzyme, to provide the textile with a dye, e.g. indigo. At least the oxidizing enzyme is a hybrid enzyme including a binding domain that is suitable to bind the enzyme to the textile and / or increase the affinity of the enzyme for the textile, in particular, a cellulose binding domain (CBD).

Disclosed in the present application is a method for preparing a regenerated cellulose fiber incorporating a recombinant protein, the method comprising the following steps: dissolving a recombinant protein and cellulose in a solvent for uniform mixing, so as to obtain a spinning solution; and using the spinning solution to perform spinning, so as to obtain a regenerated cellulose fiber incorporating the recombinant protein, the recombinant protein being a recombinant protein obtained by fusion expression of at least one cellulose binding domain and at least one functional protein. In the present application, CBM-containing recombinant functional proteins can maintain structural and functional stability in NMMO systems, so as to provide the biological activity of proteins for Lyocell fiber systems; incorporation of fluorescent proteins can impart natural fluorescence to fibers, which have application prospects in the field of textiles and fashion; expression of MaSp1 recombinant spider silk proteins enhances the fiber strength, and expression of MaSp4 recombinant spider silk proteins improves the elastic properties of fibers; and in addition, CBM fusion proteins can also carry other functional modules, so as to endow cellulose fibers with specific functionalities.

The invention relates to the technical field of coatings, and provides a coating for reducing the burning loss of a steel billet and a preparation method thereof.The coating for reducing the burning loss of the steel billet is prepared from, by weight, 15-20 parts of red mud, 10-16 parts of zirconium oxide, 2-4 parts of titanium oxide, 18-28 parts of silicon carbide, 6-9 parts of ferrous oxide, 5-8 parts of aluminum oxide, 12-17 parts of binder, 0.5-5 parts of water reducing agent and 18-28 parts of curing agent; the binder comprises the following components in parts by weight: 3-5 parts of a silica sol binder, 3-9 parts of magnesium aluminum silicate, 1-5 parts of magnesium acetylacetonate and 1-3 parts of a cellulose binder. According to the technical scheme, the problem that the effect of reducing the oxidation burning loss degree of a steel billet by a coating in related technologies is poor is solved.

Described herein is a reagent matrix comprising (a) a plurality of first capture components wherein the first capture component comprises a first analyte capture site; (b) a plurality of second capture components wherein the second capture component comprises a second analyte capture site and a cellulose binding domain (CBD) or carbohydrate binding module (CBM) wherein at least one of the first capture component or the second capture component comprises a detection medium; and (c) a plurality of cellulose nanofibrils. In one embodiment, the reagent matrix is combined with a porous substrate for detecting an analyte.

The invention provides a fusion polypeptide / bacterial cellulose composite membrane, a preparation method thereof and a wound repair material, and belongs to the field of biomedical materials. The composite hydrogel film comprises a bacterial cellulose three-dimensional network matrix, the two fusion polypeptides are specifically bound to the surface of the matrix through a cellulose binding domain, the first fusion polypeptide is hLZ-CBD with an antibacterial function, and the second fusion polypeptide is RGD-CBD with a cell adhesion proliferation promoting function. The composite hydrogel provided by the invention is mainly used for preparing a wound repair material, and the obtained composite hydrogel film has good biocompatibility and no cytotoxicity, and is suitable for long-term implantation or contact with skin. In addition, the compound shows remarkable antibacterial activity on gram-positive bacteria (such as staphylococcus aureus) and gram-negative bacteria (such as escherichia coli). In addition, the composite membrane can significantly promote proliferation and migration of fibroblasts, epithelial cells and other cells, and accelerate wound healing.

The invention belongs to the technical field of oil exploitation, and particularly relates to a clean drag reducer for fracturing and a preparation method thereof.The drag reducer is prepared from 1-10 parts of carbonized bacterial cellulose, 30-40 parts of acrylamide, 20-35 parts of itaconic acid, 0.1-0.4 part of initiator, 1-3 parts of surfactant and 50-70 parts of deionized water; the specific preparation method comprises the steps that acrylamide, itaconic acid and water are mixed, carbonized bacterial cellulose, a surfactant and an initiator are added, the mixture is heated and stirred, the drag reducer is obtained, and the surfactant is one or more of sodium alpha-olefin sulfonate, dodecyl dimethyl betaine and lauramidopropyl betaine; according to the drag reducer, bacterial cellulose is combined with acrylamide and itaconic acid, the drag reduction effect, salt resistance and temperature resistance of the drag reducer are effectively improved, a stabilizer of the drag reducer is improved, bacterial cellulose, itaconic acid and the like have good biodegradability and also have antibacterial, cleaning and scale inhibition effects, pollution to the environment is small, and the drag reducer is suitable for large-scale production. The damage to the rock core is small.

A solid state electrolyte may include a membrane including cellulose, and chitosan bound to the cellulose of the membrane. The solid state electrolyte may be provided in the form of a membrane in which base complex fibers form a network. The solid state electrolyte may contain a large amount of OH ions and moisture by the chitosan, and may have high ionic conductivity.

The present invention relates to a method for producing a concentrated phase of a silk fusion protein, the method comprising the following steps: preparing a solution of a silk fusion protein in an aqueous medium, and concentrating the silk fusion protein in the aqueous medium, wherein the fusion protein is isolated from a recombinant production host and comprises a filamentous protein sequence and two separate non-silk terminal module sequences flanking the filamentous protein sequence, such as a cellulose binding module, a SpyCatcher domain, the tenth type III module of fibronectin, and γ-crystallin D; wherein the method is performed so that the silk fusion protein is not precipitated and subsequently dissolved in the aqueous medium. The present invention also relates to the use of such a fusion protein as an adhesive.

Provided is a granule for suspension, which is a pharmaceutical composition including: a compound represented by formula 1:or a pharmaceutically acceptable salt or solvate thereof, a binding agent; and a dispersant. The binding agent includes a binding agent selected from the group consisting of a cellulose binding agent, a povidone binding agent, a vinyl alcohol binding agent, and a thickening polysaccharide binding agent. The dispersant is selected from the group consisting of an inorganic salt dispersant, an ether dispersant, a starch dispersant, a cellulose dispersant, and a fatty acid ester salt dispersant.

The present invention discloses a method for preparing a regenerated cellulose fiber combined with a recombinant protein, comprising the following steps: dissolving a recombinant protein and cellulose in a solvent and mixing them evenly to obtain a spinning solution; spinning the spinning solution to obtain the regenerated cellulose fiber combined with the recombinant protein; the recombinant protein is a recombinant protein obtained by fusion expression of at least one cellulose binding domain and at least one functional protein. The recombinant functional protein containing CBM of the present invention can maintain structural and functional stability in the NMMO system, thereby providing the biological activity of the protein for the Lyocell fiber system. The composite of fluorescent protein can bring a natural fluorescent effect to the fiber, and has application prospects in the field of textile fashion. The fiber strength is increased by expressing the MaSp1 recombinant spider silk protein, and the fiber elasticity is enhanced by expressing the MaSp4 recombinant spider silk protein. At the same time, the CBM fusion protein can also carry other functional modules to give the cellulose fiber specific functions.

The application discloses a mucor HY6-19 and application of the mucor HY6-19 in preparation of extract of red date seed, and the mucor HY6-19 is obtained by targeted separation and screening and mutagenesis, and can grow moderately in red date seed powder added with sucrose, produce cellulase with higher activity and other various hydrolytic enzymes, then, the fermented red date seed powder is added with water and kept warm, cellulose, hemicellulose and pectin and other substances in the cell wall are enzymolyzed, the substances combined with cellulose in the cell wall and intracellular substances are dissolved out, the yield of the extract is significantly improved, and the total flavone content is also significantly improved. Before preparation of the extract of the red date seed by the hot water ultrasonic method, the microbial fermentation treatment is added, compared with the direct hot water ultrasonic extraction method, the yield of the extract of the red date seed can be increased by 34.4%, and the total flavone content is increased by 23.3%.

The present invention relates to a process wherein one or more dye precursors, e.g., indole, are provided to a textile and converted by one or more enzymes, e.g., an oxidizing enzyme, to provide the textile with a dye, e.g. indigo. At least the oxidizing enzyme is a hybrid enzyme including a binding domain that is suitable to bind the enzyme to the textile and / or increase the affinity of the enzyme for the textile, in particular, a cellulose binding domain (CBD).

The invention discloses a modified bio-enzyme preparation for energy-saving enhanced pulping and application of the modified bio-enzyme preparation in pretreatment production of semi-viscous paper pulp, and belongs to the technical field of bio-enzyme and paper pulp manufacturing. The preparation method of the modified biological enzyme preparation comprises the following steps: (1) mixing a hemicellulase solution, a cellulose binding protein solution, a polysaccharide monooxygenase solution and a cellulose incision enzyme solution to obtain a composite biological enzyme solution; and (2) mixing the composite bio-enzyme solution with a chitosan solution, and adding a cross-linking agent for reaction to obtain the modified bio-enzyme preparation. The surfactant chitosan and the composite bio-enzyme are subjected to covalent cross-linking modification, and are put into wood pulp for primary pulping treatment, so that the enzymolysis efficiency and pulping performance of wood pulp fibers are remarkably improved. The method overcomes the defects of large enzyme dosage, high cost and low paper strength, solves the problems of long pulping time and high energy consumption, and has the advantages of energy conservation, environmental protection and high efficiency.

The present application discloses a composition comprising an adsorbent and a cellulose based binder that strongly adheres to a support and is flexible.

The invention belongs to the technical field of petroleum extraction, and particularly relates to a clean drag reducer for fracturing and a preparation method thereof. The drag reducer raw materials include 1-10 parts of carbonized bacterial cellulose, 30-40 parts of acrylamide, 20-35 parts of itaconic acid, 0.1-0.4 parts of an initiator, 1-3 parts of a surfactant, and 50-70 parts of deionized water. The specific preparation method comprises the following steps: mixing acrylamide, itaconic acid, and water, adding carbonized bacterial cellulose, the surfactant, and the initiator, heating and stirring to obtain the drag reducer, wherein the surfactant is one or more of sodium α-olefin sulfonate, dodecyl dimethyl betaine, and laurylamidopropyl betaine. The drag reducer effectively improves the drag reduction effect, salt resistance, and temperature resistance of the drag reducer by combining bacterial cellulose with acrylamide and itaconic acid, and improves the stabilizer of the drag reducer. The bacterial cellulose and itaconic acid have good biodegradability and also have antibacterial, clean, and scale-inhibiting effects, thereby causing little pollution to the environment and causing little damage to the core.

The present disclosure relates to a carbonic anhydrase complex and a method for preparing same, in which a conjugate of a carbonic anhydrase and a dockerin module is bound to a small cellulose binding protein including a cohesin module and a cellulose binding module (CBM) and method of manufacturing thereof. The complex, which includes a cellulose binding module, is immobilized on the surface of green algae, to increase access to a substrate and enzyme activity, thereby efficiently fixing carbon dioxide, and increasing the growth and lipid production of green algae without adding other carbon sources. The present disclosure is expected to be actively utilized in fields, such as biofuels, using carbon dioxide fixation.

The invention discloses a method for improving the extraction yield of powder-recovered ganoderma lucidum polysaccharide by combining enzymolysis with ultrasonic waves, which comprises the following steps: performing enzymolysis on powder-recovered ganoderma lucidum by using cellulase which can hydrolyze cellulose in cell walls of the powder-recovered ganoderma lucidum so as to damage the cell walls; hot water and ultrasonic waves are used for extraction, the hole effect of the ultrasonic waves can enhance the movement frequency and speed of substance molecules, the combined effect of enzymolysis and ultrasonic waves is beneficial to dissolution of polysaccharide combined with cellulose in cell walls, and the extraction yield is remarkably improved. Compared with extraction without enzymolysis, the method for extracting the powder-collected ganoderma lucidum polysaccharide through combination of enzymolysis and ultrasonic assistance has the advantage that the extraction yield of the powder-collected ganoderma lucidum polysaccharide can be increased by 82.9%.

The invention belongs to the technical field of high polymer material preparation, and provides a composite material prepared from melamine waste and a preparation method thereof. Firstly, melamine waste is subjected to steam explosion treatment to destroy the internal structure of the waste, and a citric acid solution is combined to further activate the surface of the waste; then utilizing laccase, catalase and an ionic liquid-water system to obtain detoxified waste; in matrix modification of the composite material, modified carboxylated cellulose is prepared through triethylamine-assisted silane modification reaction, and a modified polyether-ether-ketone material is obtained by combining concentrated sulfuric acid etching and silane; meanwhile, chitosan and polyethyleneimine are used as raw materials, and a functional filler of the composite material is further provided; the detoxified melamine waste, modified cellulose, modified polyether-ether-ketone and chitosan-based microspheres are combined with new melamine resin, a covalent bonding interface between the modified cellulose and a melamine matrix forms a compact three-dimensional network, formaldehyde molecule migration is limited, and the low-free-formaldehyde composite material is prepared.