10 results about "Cellulose binding" patented technology

The invention relates to a modified cellulase derived polypeptide and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The invention provides a derivative polypeptide of cellulose excision enzyme Cel7A, the cellulose excision enzyme Cel7A is derived from Trichoderma reesei, NCBI Acession No.XM006969162.1, and the amino acid sequence of the cellulose excision enzyme Cel7A is as shown in SEQ ID NO: 1. The invention further provides a preparation method of the derivative polypeptide of the cellulose excision enzyme Cel7A. Wherein the amino acid sequence of the cellulose binding domain CBM is as shown in SEQ ID NO: 2. The derivative polypeptide is a polypeptide of which the amino acid sequence is 70% or more identical to that of a polypeptide shown in SEQ ID NO: 2, can reduce combination with lignin and improve the degradation efficiency of cellulose, so that the saccharification efficiency of cellulase is improved, the post-treatment process cost is greatly saved, and the derivative polypeptide is suitable for industrial production. The method has important industrial application value.

The present application relates to the field of hydrogel 3D printing, flexible sensor, wearable electronic device, in particular to a cellulose-based hydrogel meeting 3D printing and sensing performance, and a preparation method and application thereof.The cellulose-based hydrogel contains zinc salt, lithium salt, water and alkali pretreated cellulose;in the cellulose-based hydrogel, the content of the alkali pretreated cellulose is 1%-6% based on 100% of the total mass of the zinc salt, lithium salt and water, the molar ratio of the total amount of zinc salt and lithium salt to water is 1:(3-4), the molar ratio of zinc salt to lithium salt is 1:(0.4-2.4), and the molar amount of zinc salt and lithium salt is calculated based on the content of zinc element and lithium element.The present application uses alkali pretreated cellulose, combines the ratio of alkali pretreated cellulose and metal salt solution (containing the above-mentioned zinc salt, lithium salt and water) with a specific content, and the composition of the metal salt solution, to obtain a cellulose-based hydrogel meeting sensing and 3D printing performance, and having better anti-freezing performance, higher conductivity and better mechanical performance, achieving unexpected technical effects.

Disclosed in the present application is a method for preparing a regenerated cellulose fiber incorporating a recombinant protein, the method comprising the following steps: dissolving a recombinant protein and cellulose in a solvent for uniform mixing, so as to obtain a spinning solution; and using the spinning solution to perform spinning, so as to obtain a regenerated cellulose fiber incorporating the recombinant protein, the recombinant protein being a recombinant protein obtained by fusion expression of at least one cellulose binding domain and at least one functional protein. In the present application, CBM-containing recombinant functional proteins can maintain structural and functional stability in NMMO systems, so as to provide the biological activity of proteins for Lyocell fiber systems; incorporation of fluorescent proteins can impart natural fluorescence to fibers, which have application prospects in the field of textiles and fashion; expression of MaSp1 recombinant spider silk proteins enhances the fiber strength, and expression of MaSp4 recombinant spider silk proteins improves the elastic properties of fibers; and in addition, CBM fusion proteins can also carry other functional modules, so as to endow cellulose fibers with specific functionalities.

The invention relates to the technical field of coatings, and provides a coating for reducing the burning loss of a steel billet and a preparation method thereof.The coating for reducing the burning loss of the steel billet is prepared from, by weight, 15-20 parts of red mud, 10-16 parts of zirconium oxide, 2-4 parts of titanium oxide, 18-28 parts of silicon carbide, 6-9 parts of ferrous oxide, 5-8 parts of aluminum oxide, 12-17 parts of binder, 0.5-5 parts of water reducing agent and 18-28 parts of curing agent; the binder comprises the following components in parts by weight: 3-5 parts of a silica sol binder, 3-9 parts of magnesium aluminum silicate, 1-5 parts of magnesium acetylacetonate and 1-3 parts of a cellulose binder. According to the technical scheme, the problem that the effect of reducing the oxidation burning loss degree of a steel billet by a coating in related technologies is poor is solved.

The invention provides a fusion polypeptide / bacterial cellulose composite membrane, a preparation method thereof and a wound repair material, and belongs to the field of biomedical materials. The composite hydrogel film comprises a bacterial cellulose three-dimensional network matrix, the two fusion polypeptides are specifically bound to the surface of the matrix through a cellulose binding domain, the first fusion polypeptide is hLZ-CBD with an antibacterial function, and the second fusion polypeptide is RGD-CBD with a cell adhesion proliferation promoting function. The composite hydrogel provided by the invention is mainly used for preparing a wound repair material, and the obtained composite hydrogel film has good biocompatibility and no cytotoxicity, and is suitable for long-term implantation or contact with skin. In addition, the compound shows remarkable antibacterial activity on gram-positive bacteria (such as staphylococcus aureus) and gram-negative bacteria (such as escherichia coli). In addition, the composite membrane can significantly promote proliferation and migration of fibroblasts, epithelial cells and other cells, and accelerate wound healing.

A solid state electrolyte may include a membrane including cellulose, and chitosan bound to the cellulose of the membrane. The solid state electrolyte may be provided in the form of a membrane in which base complex fibers form a network. The solid state electrolyte may contain a large amount of OH ions and moisture by the chitosan, and may have high ionic conductivity.

Provided is a granule for suspension, which is a pharmaceutical composition including: a compound represented by formula 1:or a pharmaceutically acceptable salt or solvate thereof, a binding agent; and a dispersant. The binding agent includes a binding agent selected from the group consisting of a cellulose binding agent, a povidone binding agent, a vinyl alcohol binding agent, and a thickening polysaccharide binding agent. The dispersant is selected from the group consisting of an inorganic salt dispersant, an ether dispersant, a starch dispersant, a cellulose dispersant, and a fatty acid ester salt dispersant.

The application discloses a mucor HY6-19 and application of the mucor HY6-19 in preparation of extract of red date seed, and the mucor HY6-19 is obtained by targeted separation and screening and mutagenesis, and can grow moderately in red date seed powder added with sucrose, produce cellulase with higher activity and other various hydrolytic enzymes, then, the fermented red date seed powder is added with water and kept warm, cellulose, hemicellulose and pectin and other substances in the cell wall are enzymolyzed, the substances combined with cellulose in the cell wall and intracellular substances are dissolved out, the yield of the extract is significantly improved, and the total flavone content is also significantly improved. Before preparation of the extract of the red date seed by the hot water ultrasonic method, the microbial fermentation treatment is added, compared with the direct hot water ultrasonic extraction method, the yield of the extract of the red date seed can be increased by 34.4%, and the total flavone content is increased by 23.3%.

The present application discloses a composition comprising an adsorbent and a cellulose based binder that strongly adheres to a support and is flexible.

The present disclosure relates to a carbonic anhydrase complex and a method for preparing same, in which a conjugate of a carbonic anhydrase and a dockerin module is bound to a small cellulose binding protein including a cohesin module and a cellulose binding module (CBM) and method of manufacturing thereof. The complex, which includes a cellulose binding module, is immobilized on the surface of green algae, to increase access to a substrate and enzyme activity, thereby efficiently fixing carbon dioxide, and increasing the growth and lipid production of green algae without adding other carbon sources. The present disclosure is expected to be actively utilized in fields, such as biofuels, using carbon dioxide fixation.