145 results about "Cellulose binding" patented technology

A polysaccharide conjugate comprises a polysaccharide with an attached entity having a molecular weight of at least 5000, the polysaccharide conjugate being capable of binding to cellulose. Preferred polysaccharides include tamarind seed xyloglucan, locust bean gum and enzyme modified guar. The attached entity is suitably a protein such as an enzyme, antibody or antibody fragment, or a particle possibly having a benefit agent such as a fragrance associated therewith. Because the polysaccharide conjugate binds to cellulose, which is present in cotton and other fabrics, paper, etc., binding of the conjugate to cellulose brings the attached entity into close proximity to a surface of or containing cellulose. The invention thus enables targeting of attached entities to such surfaces. The invention also provides a product incorporating the polysaccharide conjugate of the invention. The product is conveniently a laundry product such as a fabric washing product, e.g. a detergent product, or a fabric conditioning product. In this case the attached entity may be an enzyme, a particle bearing fragrance, etc. The invention also provides a method of targeting binding of an entity to cellulose by use of the polysaccharide conjugate of the invention.

A process of expressing a recombinant protein in a plant and of isolating the recombinant protein from the plant, the process is effected by (a) providing a plant, a plant derived tissue or cultured plant cells expressing a fusion protein including the recombinant protein and a cellulose binding peptide being fused thereto, the fusion protein being compartmentalized within cells of the plant, plant derived tissue or cultured plant cells, so as to be sequestered from cell walls of the cells of the plant, plant derived tissue or cultured plant cells; (b) homogenizing the plant, plant derived tissue or cultured plant cells, so as to bring into contact the fusion protein with a cellulosic matter of the plant, plant derived tissue or cultured plant cells, to thereby effect affinity binding of the fusion protein via the cellulose binding peptide to the cellulosic matter, thereby obtaining a fusion protein cellulosic matter complex; and (c) isolating the fusion protein cellulosic matter complex.

The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose. These systems have a number of applications.

The invention provides a method for pretreating cotton fabrics by adopting glucoamylase/glucose oxidase, pectinase and CBD (cellulose-binding domain)-lignin peroxidase, belonging to the technical field of cotton fabric dyeing and finishing pretreatment. The method is characterized in that the cotton fabrics are firstly treated in mixed enzyme solution containing the glucoamylase and the glucose oxidase and the glucoamylase catalyzes the starch size on the fabrics to be decomposed into glucose so as to eliminate the size; the glucose oxidase catalyzes the glucose and oxygen to act to generate hydrogen peroxide; the generated hydrogen peroxide not only plays a role of bleaching but also can damage the hydrophobic surface structure of the cotton to expose the lower pectic substances; then the cotton fabrics are treated in alkaline pectinase solution to remove the pectic substances through decomposition; and finally the cotton fabrics are treated in CBD-lignin peroxidase solution to finally remove the cotton seed hulls through decomposing the lignin in the cotton seed hulls. The method provided by the invention can replace the traditional chemical treatment method to realize full bio-enzyme pretreatment of the cotton fabrics, and at the same time the traditional hydrogen peroxide can be no longer adopted for bleaching.

The present invention relates to a method for detecting protein-protein interactions in living cells, and more particularly, to a method for providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises a bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises a prey protein and a second fluorescent protein so as to allow formation of inclusion bodies, and detecting interactions between the bait protein and the prey protein that are displayed by inclusion bodies, a method for isolating the prey protein bound to the bait protein using the cells comprising the constructs, the cells, and a kit for detecting protein-protein interactions, comprising the constructs.

The present invention relates to methods for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain. The present invention also relates to such cellulolytic enzyme compositions.

The invention relates to a high glucose resistance beta-glucosidase-CBD fusion enzyme, and expression gene and application thereof. The amino acid sequence is shown as SEQ ID NO. 1. Compared with the conventional beta-glucosidase enzyme, the fusion enzyme (BGL-CBD) provided by the present invention has the following characteristics: (1) higher cellulose binding ability, wherein the fusion enzyme (1 [mu]g/[mu]L) is incubated with 4 wt% of microcrystalline cellulose for 30min, and 98% of fusion enzyme is adsorbed by cellulose; (2) effectively degrading cellobiose, wherein the degradation rate of 10 wt% of the cellobiose by the fusion enzyme (0.15 [mu]g/[mu]L) within 8 h is 95%; and (3) resistance for high concentration glucose, wherein the glucose tolerance factor Ki is 1000 mmol/L.

Cellulase fusion proteins comprising an endoglucanase core region and a heterologous cellulose binding domain are described. The fusion proteins may be produced by recombinant techniques using appropriate polynucleotides, expressing vectors and host cells. The fusion proteins and enzyme preparations thereof are useful in treating cellulosic material, such as textile material, and they are particularly useful in biostoning denim or in biofinishing fabrics and garments. In addition the fusion proteins may be used in pulp and paper industry, oil extraction from plants, detergent compositions, or for improving the quality of animal feed.

The invention discloses a method for preparing heat-resistant cutinase-CBD and application thereof to cotton fiber refining, which belong to the field of enzyme gene engineering. The method comprises the steps: taking pET20b-Tfu_0883 plasmid DNA and total DNA of thermophilic ascomycete WSH03-11 as a template, performing PCRs of designed primers respectively to obtain an encoding cutinase Tfu_0883 gene and a CBD gene in cellulose Tfu_1074, and then obtaining a fusion gene of encoding cutinase-cellulose-binding domain (CBD) (shortened as the heat-resistant cutinase-CBD) through overlap PCR; and taking pET20b(+) as an expression vector and E.coli BL21(DE3) as an expression host to realize the high level expression of a heat-resistant cutinase-CBD gene. The fusion enzyme has the activity of cutinase, the optimum temperature is 50 DEG C, the optimum pH value is 8, and the activity of the enzyme can still reach over 50 percent when heat preservation for 50h at a temperature of 50 DEG C. The cutinase-CBD has an absorption rate of 50 percent on cotton fiber, reaches reaction balance after 6h, and generates 180mu mol of fatty acid. The fusion enzyme is suitable for cotton fiber refining.

The invention discloses a novel fusion protein of a mispairing binding protein and a cellulose binding domain 3, establishes a method for rapidly immobilizing the fusion protein on cellulose, and a method for constructing a mispairing binding protein cellulose chromatographic column. The invention further establishes a method for removing errors in oligonucleotides synthesized by a chip at high flux by using the mispairing binding protein cellulose chromatographic column. Meanwhile, the method disclosed by the invention is capable of correcting the errors in the DNA synthesis at high flux.

The invention provides a method for scouring a cotton fabric by applying cutinase and CBD (cellulose-binding domain)-alkaline pectinase, belonging to the technical field of treatment of cotton fabrics before dyeing and finishing. The method comprises the following steps of: treating a cotton knitted fabric, cotton yarns, or a cotton woven fabric from which size is removed by amylase or sodium hydroxide firstly, in a mixed enzyme solution formed by cutinase and CBD-alkaline pectinase; or respectively treating in the cutinase solution and the CBD-pectinase solution, wherein the hydrophobic surface structure of cotton fibers is destructed by the cutinase, the higher fatty acid ester substances are removed from the surface waxy layers of the cotton fibers, so that the wettability of the cotton fabric is improved, the pectic substances on the cotton fibers are exposed, and then the CBD-pectinase is absorbed on the surface cellulose of the cotton fibers to decompose the pectic substances on the cotton fibers. By the method provided by the invention, on the one hand, the traditional chemical treatment method can be substituted for scouring the cotton fabric, and on the other hand, because the cutinase and the CBD-alkaline pectinase are adopted, the wettability of the cotton fibers and the pectin removal rate are improved.

The invention provides micro-nano lignocellulose, a preparation method and applications thereof. The micro-nano lignocelluloses contain lignin structures; lignin is bonded with cellulose through hydrogen bonds and chemical bonds. The preparation method comprises following steps: a lignin containing cellulose raw material is dispersed in a hot urea aqueous solution so as to obtain a cellulose raw material dispersion liquid; mechanical pre-treatment is adopted for peeling and grinding so as to obtain a pre-treatment product; and the pre-treatment product is subjected to high pressure homogenizing using a high pressure homogenizer so as to obtain a micro-nano lignocelluloses dispersion liquid. According to the preparation method, removing of the lignin in the lignin containing cellulose raw material is not needed, the hot urea aqueous solution is combined with mechanical pre-treatment and high pressure homogenizing so as to prepare the product; the diameter of the obtained micro-nano lignocelluloses ranges from 5 to 180nm; the length/diameter ratio is 200 or larger; the lignin content ranges from 10 to 35wt%; micro-nano cellulose performance is improved; and the application prospect is promising.

The invention relates to a preparation method of flame-retardant Lyocell fiber. The preparation method is characterized in that flame retardants of tris-(2,3-dibromopropyl)phosphate and cotton pulp are dissolved in an N-methylmorpholine-N-oxide water solution; a spinning solution is prepared; the spinning solution enters a spinning system; sprayed silk threads are vertically stretched in air and enter a coagulating bath tank to be coagulated into fiber; the fiber is subjected to the processes such as alcohol washing, blanching, water washing, oiling and drying; the flame-retardant Lyocell fiber is obtained. The dry breaking strength intensity of the prepared Lyocell fiber is 43 to 45 cN/tex, and the dry breaking elongation is 13.5 to 15 percent; the wet breaking strength of the Lyocell fiber is 31.5 to 33 cN/tex, and the wet breaking elongation of the fiber is 15 to 17 percent; the major strong performance approaches to that of commercially-available Lyocell fiber. The limit oxygen index of the prepared flame-retardant Lyocell fiber reaches 33 percent or higher; after water washing for 20 times, the flame-retardant effect is still not obviously weakened; the result shows that the combining force of the flame retardant and cellulose is high.

A recombinant vector for transforming a plant, a plant transformed with the recombinant vector, a plant-made classical swine fever virus antigen pmE2 protein expressed in the plant and uses thereof is provided. By using a recombinant vector having a polynucleotide encoding a GP55 protein of CSFV according to the present invention; and a polynucleotide encoding a cellulose-binding domain protein; and a plant transformed with the recombinant vector, a plant-made classical swine fever virus antigen pmE2 protein may be produced with high efficiency, and has higher safety and stability than those achieved by other production methods. Also, since the plant-derived classical swine fever virus antigen protein pmE2 has a cellulose-binding domain (CBD) protein, it may be usefully used as an effective marker to determine a virus exposure pathway and an antibody producing pathway.

The present invention relates to fabric care compositions comprising one or more amino acid sequence(s) comprising a Cellulose Binding Domain for providing fabric care. In a further embodiment, the present invention relates to fabric care compositions wherein the amino acid sequence comprising one or more cellulose binding domains is linked to a softening protein.