The invention relates to a modified
cellulase derived polypeptide and application thereof, and belongs to the technical field of
gene engineering and
enzyme engineering. The invention provides a derivative polypeptide of
cellulose excision
enzyme Cel7A, the
cellulose excision
enzyme Cel7A is derived from
Trichoderma reesei, NCBI Acession No.XM006969162.1, and the
amino acid sequence of the
cellulose excision enzyme
Cel7A is as shown in SEQ ID NO: 1. The invention further provides a preparation method of the derivative polypeptide of the cellulose excision enzyme Cel7A. Wherein the
amino acid sequence of the
cellulose binding domain CBM is as shown in SEQ ID NO: 2. The derivative polypeptide is a polypeptide of which the
amino acid sequence is 70% or more identical to that of a polypeptide shown in SEQ ID NO: 2, can reduce combination with
lignin and improve the degradation efficiency of cellulose, so that the saccharification efficiency of
cellulase is improved, the post-treatment process cost is greatly saved, and the derivative polypeptide is suitable for industrial production. The method has important industrial application value.