The invention belongs to the field of
biological materials and
tissue engineering, and provides a preparation method and application of a multistage bionic
periosteum capable of regulating and controlling an osteogenesis microenvironment, oxidized
xyloglucan is dissolved in water, BMP2 is added for mixing reaction, and an OXG-BMP2 solution is obtained; the preparation method comprises the following steps: adding an OXG-BMP2 solution into
dichloromethane, dropwise adding an emulsifier, and stirring to obtain an
emulsion containing OXG-BMP2 microparticles; adding L-
polylactic acid and N, N-
dimethylformamide into the
emulsion containing the OXG-BMP2 microparticles, and obtaining a core-shell structure spun
yarn by using an electrostatic
spinning technology; the method comprises the following steps: mixing TA-Ce nano-
enzyme into I-type
rat tail collagen, uniformly mixing, dropwise adding into core-shell structure
spinning, and incubating to complete self-
assembly of the bionic
periosteum, thereby obtaining the multi-stage bionic
periosteum capable of regulating and controlling the osteogenesis microenvironment. The multi-stage bionic periosteum can regulate and control the osteogenesis microenvironment and sequentially promote osteogenesis, and has great application prospects in the aspects of
bone injury repair and the like.