Tumor microenvironment dual-response drug controlled-release microcapsule based on natural polysaccharide and application thereof

A tumor microenvironment and drug controlled release technology, applied in the field of biomedical polymer materials, can solve the problem of uncontrollable release rate and achieve the effect of cheap raw materials, strong stability, and mild reaction conditions

Active Publication Date: 2018-09-25
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(Colloids and Surfaces B: Biointerfaces, 2016, 142, 281-289.) Usually, the drug release of these microcapsules is achieved by hydrolysis or enzymatic hydrolysis of the film, and...

Method used

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  • Tumor microenvironment dual-response drug controlled-release microcapsule based on natural polysaccharide and application thereof
  • Tumor microenvironment dual-response drug controlled-release microcapsule based on natural polysaccharide and application thereof
  • Tumor microenvironment dual-response drug controlled-release microcapsule based on natural polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Using CaCO 3 Nanoparticles and XG-MA, XG-BrMA and XG-SH with functionalities of 20%, 50% and 40%, respectively. CaCO 3 Nanoparticles were dispersed into the chloroform solution of the anticancer drug, immersed for 15 minutes, centrifuged, the supernatant was removed, and the precipitate was dried for later use. Drug-loaded CaCO 3 The nanoparticles are placed in an aqueous solution of mercaptoethylamine hydrochloride, reacted for 25 minutes, and then centrifuged to obtain a thiol template loaded with anticancer drugs.

[0029] Place the thiolated template in the aqueous solution of XG-(Br)MA, vortex to disperse evenly, centrifuge after 10 minutes of reaction, remove the supernatant, wash with deionized water for 3 times, and then place the microspheres in XG-SH In the aqueous solution, the second layer was deposited by the same method, the microspheres were washed twice with deionized water, and then the above LbL assembly process was repeated, and XG-(Br)MA and XG-SH...

Embodiment 2

[0032] Using CaCO 3 Nanoparticles and XG-MA, XG-BrMA and XG-SH with functionalities of 30%, 50% and 30%, respectively. CaCO 3 Nanoparticles were dispersed into the chloroform solution of anticancer drugs, immersed for 20 minutes, centrifuged, the supernatant was removed, and the precipitate was dried for later use. Drug-loaded CaCO 3 The nanoparticles are placed in an aqueous solution of mercaptoethylamine hydrochloride, reacted for 15 minutes, and then centrifuged to obtain a thiol template loaded with anticancer drugs.

[0033] Place the thiol-modified microspheres in the aqueous solution of XG-(Br)MA, vortex to disperse evenly, centrifuge after 25 minutes of reaction, remove the supernatant, wash twice with deionized water, and continue to place the microspheres in the thiol In the aqueous solution of the xyloglucan derivative (XG-SH) of the group, the second layer was deposited by the same method, the microspheres were washed with deionized water for 4 times, and the ab...

Embodiment 3

[0036] Using CaCO 3Nanoparticles and XG-MA, XG-BrMA and XG-SH with functionalities of 50%, 10% and 60%, respectively. CaCO 3 Nanoparticles were dispersed into the chloroform solution of anticancer drugs, immersed for 30 minutes, centrifuged, the supernatant was removed, and the precipitate was dried for later use. Drug-loaded CaCO 3 The nanoparticles were placed in an aqueous solution of mercaptoethylamine hydrochloride, reacted for 25 minutes, and then centrifuged. Obtain sulfhydryl templates loaded with anticancer drugs.

[0037] Place the thiol-modified microspheres in an aqueous solution of XG-(Br)MA, vortex to disperse evenly, centrifuge after 10 minutes of reaction, remove the supernatant, wash with deionized water for 4 times, and then continue to place the microspheres in the thiol In the aqueous solution of the xyloglucan derivative (XG-SH) of the group, the second layer was deposited by the same method, the microspheres were washed with deionized water for 3 time...

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Abstract

The invention discloses a tumor microenvironment dual-response drug controlled-release microcapsule based on a natural polysaccharide and application thereof. A capsule shell layer is of a multilayered structure and layers are connected through a chemical bond; each layer is composed of a succinimide thioether bonding structure formed by assembling XG-MA/XG-SH or a thiomaleimide bonding structureformed by assembling XG-BrMA/XG-SH; the capsule shell layer covers an anti-tumor drug. The invention adopts a construction method of the microcapsule with certain stability based on natural xyloglucan; the layers of the microcapsule are connected through the chemical bond and have relatively high stability; meanwhile, the microcapsule has dual-response performance on a tumor microenvironment; reduction and cracking rates of the succinimide thioether bonding structure and the thiomaleimide bonding structure in the microcapsule are different; the controlled-release rate of the anti-tumor drug can be realized though regulating and controlling the ratio of the XG-MA to the XG-BrMA.

Description

technical field [0001] The invention relates to the field of biomedical polymer materials, in particular to a natural polysaccharide-based tumor microenvironment microcapsule with dual responsiveness to pH and reduction and its application in controlled release of anticancer drugs. Background technique [0002] Polymer microcapsules have application prospects in many fields such as controlled drug release, microreactors, sensors, and catalysis. The methods for preparing polymer microcapsules mainly include template surface layer-by-layer assembly (layer-by-layer, LbL), block copolymer self-assembly, emulsion polymerization, template surface-initiated polymerization and other methods. The LbL method is one of the commonly used methods for constructing microcapsules. It mainly assembles functional polymers on the surface of three-dimensional colloidal particles into a multilayer film structure based on the effects of electrostatic, hydrogen bonding, and covalent bonding. After...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K9/50A61K47/36A61K31/704A61P35/00
CPCA61K9/5036A61K31/704A61P35/00
Inventor 徐婷陈静张华高国荣付俊
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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