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A kind of nanocomposite graphene hydrogel system for breast cancer combined chemotherapy and preparation method thereof
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A graphene hydrogel and nanocomposite technology, applied in the field of nanomaterials, to achieve simple operation, mild experimental conditions, and good biocompatibility
Active Publication Date: 2019-05-07
JINLING INST OF TECH
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[0008] So far, there have been no reports of nanocomposite graphene hydrogels simultaneously carrying two anticancer drugs, epirubicin (hydrophilic) and paclitaxel (hydrophobic), and used in combination chemotherapy for breast cancer.
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Embodiment 1
[0047] Step 1: Preparation of graphene system loaded with paclitaxel alone (PTX@GN)
[0048] Add 0.12g of GO to 100mL of 1% CTAB distilled aqueous solution, ultrasonically disperse in ice bath for 30min, stir for 6h, at 10000r min -1 Centrifuge and wash with distilled water several times. Mix the product with 200mL of 0.24g of PSS solution, ultrasonically disperse for 30min, stir for 6h, continue to wash with distilled water for 2 to 3 times, and dry in vacuum at 40°C. The obtained sample is the modified graphene oxide f- go.
[0049] Add 400 mg of PF127 to 15 mL of 1.0 mg / mL f-GO aqueous solution, add 400 μL of hydrazine hydrate under stirring at room temperature, and ultrasonically stir at 40 °C for 24 h to reduce graphene oxide (GO) to graphene (GN ). After the reaction, the mixture was filtered to obtain the GN modified by PF127. GN modified by PF127 was added to 30 mL of 1 mg / mL PTX buffer in PBS, stirred at 25°C in the dark for 16 h, centrifuged and washed, and freez...
Embodiment 2
[0058] In order to better conduct comparative experiments, in step 2, the GN modified by PF127 can be directly used as an additive to synthesize nanocomposite graphene hydrogel GDL without paclitaxel, and then the experiment of loading epirubicin can be carried out, and the loading table can be obtained. Nanocomposite graphene hydrogel drug-loading system (EPI@GDL) with doxorubicin as a single drug.
[0059]Step 1: Preparation of PF127 modified graphene system (blank GN carrier)
[0060] Add 0.12g of GO to 100mL of 1% CTAB distilled aqueous solution, ultrasonically disperse in ice bath for 30min, stir for 6h, at 10000r min -1 Centrifuge and wash with distilled water several times. Mix the product with 200mL of 0.24g of PSS solution, ultrasonically disperse for 30min, stir for 6h, continue to wash with distilled water for 2 to 3 times, and dry in vacuum at 40°C. The obtained sample is the modified graphene oxide f- go.
[0061] Add 400 mg of PF127 to 15 mL of 1.0 mg / mL f-GO ...
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Abstract
The invention discloses a preparation method of a nanometer composite graphene hydrogel system having pH / temperature dual response and simultaneously carrying breast cancer combined chemotherapy drugs comprising paclitaxel and epirubicin. The method comprises the following steps: 1, preparing hydrophobic drug paclitaxel supported graphene PTX@GX; 2, carrying out a free radical polymerization reaction through adopting the PTX@GX as an additive and selecting temperature-sensitive N-isopropylacrylamide NIPAM monomer as first network to prepare single network hydrogel, and carrying out ultraviolet irradiation by selecting pH-responsive acrylic acid AA monomer as a second network in order to synthesize composite graphene double network hydrogel with pH / temperature dual response; 3, loading a hydrophilic drug epirubicin EPI to obtain a PTX and EPI dual supported nanometer composite graphene hydrogel double drug-loading system EPI@PTX@GDL; and 4, detecting the cytotoxicity by using the prepared drug-loading system through an MTM technology, and observing the form of living cells by using a fluorescence microscope. The invention also discloses an application of the prepared composite drug loading system in breast cancer therapy.
Description
technical field [0001] The invention relates to the field of nanomaterials and biotechnology, in particular to a nanocomposite graphene hydrogel system with dual responsiveness and combined chemotherapy for breast cancer and a preparation method thereof. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. In my country, breast cancer accounts for 7% to 10% of all kinds of malignant tumors in the whole body, and the incidence rate is about 18.7 per 100,000 people. [0003] Paclitaxel (PTX) is a diterpenoid compound isolated from the bark of Taxus brevifolia. It has poor water solubility and is a hydrophobic drug. It is a new type of microtubule stabilizer, which can prevent the proliferation of tumor cells by inducing and promoting tubulin polymerization and stabilizing microtubules. Clinical studies have shown that paclitaxel has a good curative effect in treating breast cancer. Epirubicin (epirubicin, EPI) is a semi-synthetic de...
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