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Method for extracting xyloglucan from tamarind seeds

A technology for xyloglucan and tamarind polysaccharide, which is applied in the field of plant polysaccharide extraction, can solve the problems of restricting the application of tamarind xyloglucan, poor solubility and low purity, and achieves the effects of high purity, promotion of application and reduction of processes

Active Publication Date: 2022-03-15
QINGDAO CHENLAND MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As for the extraction methods of polysaccharides, there are classic water extraction and alcohol precipitation method and organic acid extraction method, but the polysaccharides prepared by these two methods have the disadvantages of poor solubility and low purity, which greatly limit the production capacity of tamarind xylogluglucans. The application of sugar in the field of biomedicine

Method used

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  • Method for extracting xyloglucan from tamarind seeds
  • Method for extracting xyloglucan from tamarind seeds

Examples

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Effect test

Embodiment 1

[0029] A method for extracting xyloglucan in tamarind seeds, the steps are as follows:

[0030] (1) Tamarind seeds are pretreated to obtain tamarind polysaccharide coarse powder, and tamarind polysaccharide coarse powder is prepared into a 1% solution with water and dissolved at 80° C. for 6 hours;

[0031] (2) Centrifuge at 9000rpm for 15min, and get the supernatant, which is the tamarind polysaccharide solution;

[0032] (3) Add 0.5% lactic acid to the tamarind polysaccharide solution, adjust the pH to 3.5, then let it settle for 3 hours at room temperature, and then centrifuge to obtain the supernatant I;

[0033] (4) Add 0.1% sodium hydroxide to the supernatant I, adjust the pH to 11, let it settle for 2 hours, and centrifuge to obtain the supernatant II;

[0034] (5) Add 2% activated carbon to the supernatant II and stir for 4 hours for adsorption, and obtain the supernatant III after centrifugation;

[0035] (6) Put the supernatant III into a 50kDa dialysis bag in a st...

Embodiment 2

[0039] A method for extracting xyloglucan in tamarind seeds, the steps are as follows:

[0040] (1) Tamarind seeds are pretreated to obtain tamarind polysaccharide coarse powder, and tamarind polysaccharide coarse powder is prepared into a 3% solution with water, and dissolved at 90° C. for 4 hours;

[0041] (2) Centrifuge at 9000rpm for 15min, and get the supernatant, which is the tamarind polysaccharide solution;

[0042] (3) Adding 1% acetic acid to the tamarind polysaccharide solution, adjusting the pH to 4.2, then standing still at room temperature for 6 hours, and then centrifuging to obtain the supernatant I;

[0043] (4) Add 1% potassium hydroxide to the supernatant I to adjust the pH to 12.1, then let it settle for 4 hours at room temperature, and then centrifuge to obtain the supernatant II;

[0044] (5) Add 2% activated carbon to the supernatant II and stir for 4 hours for adsorption, and obtain the supernatant III after centrifugation;

[0045] (6) Put the supern...

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Abstract

The invention discloses a method for extracting xyloglucan from tamarind seeds, and belongs to the technical field of plant polysaccharide extraction. According to the diversity of protein types in tamarind seeds and the characteristics of acid resistance, salt resistance and heat resistance of tamarind xyloglucan, the tamarind seeds are pretreated to obtain tamarind polysaccharide coarse powder, and then protein in polysaccharide is removed through acid treatment and alkali treatment in sequence in combination with activated carbon adsorption. And dialyzing under a sterile condition to finally prepare the ultra-pure tamarind xyloglucan with low protein content and low bacterial endotoxin content. Compared with an existing water extraction and alcohol precipitation method and an organic acid extraction method for extracting tamarind xyloglucan, the polysaccharide prepared through the method has the advantages of being good in solubility, high in purity and the like, application of the tamarind xyloglucan in the field of biological medicine is greatly promoted, alcohol is not needed to serve as a settling agent, procedures are reduced, and cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of plant polysaccharide extraction, and in particular relates to a method for extracting xyloglucan from tamarind seeds. Background technique [0002] Xyloglucan (XG) in tamarind seeds is a natural, non-toxic, edible polysaccharide. Tamarind is mainly produced in India, Myanmar, Pakistan and other places, and it is also planted in Yunnan, Guangdong, Fujian and other provinces in my country. Tamarind xyloglucan is mainly composed of D-galactose, D-xylose and D-glucose, and its relative molecular mass varies greatly due to different measurement methods, about 250,000-650,000. In the molecular structure of tamarind xyloglucan, the main chain is composed of D-glucopyranose linked by β-1,4 glycosidic bonds, and the side chain is D-xylopyranose linked by α-1,6 glycosidic bonds D-galactopyranose linked to β-1,2 glycosidic bonds. Tamarind xyloglucan has a stabilizing effect on salt resistance, heat resistance and...

Claims

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Application Information

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IPC IPC(8): C08B37/14
CPCC08B37/0003C08B37/0024
Inventor 邹圣灿刘桂亭孙婷王宝群林莎莎
Owner QINGDAO CHENLAND MARINE BIOTECH CO LTD
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