Paenibacillus xylanilyticus strain and method for preparing xyloglucanase by using same

A technology of xyloglucanase and bacillus, which is applied in the field of microorganisms and enzyme production, can solve the problems of difficult control of production conditions, difficult separation and purification of products, and low enzyme production level, so as to achieve easy control of production conditions, easy production of products, The effect of high enzyme production levels

Active Publication Date: 2012-10-31
无锡泰仑达化机设备有限公司
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Enzymes can be produced by microbial fermentation, but there are few microorganisms producing xyloglucanase reported in the prior art, and there are disad...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paenibacillus xylanilyticus strain and method for preparing xyloglucanase by using same
  • Paenibacillus xylanilyticus strain and method for preparing xyloglucanase by using same
  • Paenibacillus xylanilyticus strain and method for preparing xyloglucanase by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The specific steps for preparing xyloglucanase using Paenibacillus xylanolyticum B20CCTCC M 2012149 are as follows:

[0036] (1) Activation of strains on the slant: inoculate the Paenibacillus xylanolyticum B20CCTCC M 2012149 on the slant medium by streaking and inoculating it at 28°C for 24 hours. Paste 4, NaCl 12, agar 22, the remaining ingredients are water, adjust the pH value to 6.5, and autoclave at 115°C for 20 minutes;

[0037] (2) Seed culture: Scrape off the 1 cm2 area of ​​bacteria grown on the slant medium in step (1), inoculate in liquid activation medium, shake and cultivate at 28°C for 12 hours, shaker speed 100r / min, liquid The composition of the seed medium is calculated in g / L: peptone 8, yeast extract 4, NaCl 8, xylose 2, and the rest is water, adjust the pH value to 6.0, and autoclave at 115°C for 20 minutes;

[0038] (3) Liquid fermentation culture: Inoculate the inoculum of the activated seed solution obtained through step (2) with a volume percen...

Embodiment 2

[0041] The specific steps for preparing xyloglucanase using Paenibacillus xylanolyticum B20CCTCC M 2012149 are as follows:

[0042] (1) Activation of slant strains: the Paenibacillus xylanolyticum B20CCTCC M 2012149 was inoculated on the slant medium by streaking and cultured at 29°C for 26 hours. Paste 5, NaCl 11, agar 21, the remaining ingredients are water, adjust the pH value to 6.6, and autoclave at 115°C for 20 minutes;

[0043] (2) Seed culture: Scrape off the 1 cm2 area of ​​bacteria grown on the slant medium in step (1), inoculate in liquid activation medium, shake and cultivate at 29°C for 13 hours, shaker speed 150r / min, liquid The composition of the seed medium is calculated in grams / liter: peptone 9, yeast extract 5, NaCl 9, xylose 3, and the rest is water, adjust the pH value to 6.2, and autoclave at 115°C for 20 minutes;

[0044] (3) Liquid fermentation culture: Inoculate the inoculum of the activated seed solution obtained through step (2) with an inoculum vol...

Embodiment 3

[0047] The specific steps for preparing xyloglucanase using Paenibacillus xylanolyticum B20CCTCC M 2012149 are as follows:

[0048] (1) Activation of slant strains: the Paenibacillus xylanolyticum B20CCTCC M 2012149 was inoculated on the slant medium by streaking and cultured at 30°C for 30 hours. Paste 6, NaCl 10, agar 20, the remaining ingredients are water, adjust the pH value to 6.7, and autoclave at 115°C for 20 minutes;

[0049] (2) Seed culture: Scrape off the 1 cm2 area of ​​bacteria grown on the slant medium in step (1), inoculate in liquid activation medium, shake and cultivate at 30°C for 14 hours, shaker speed 200r / min, liquid The composition of the seed medium is calculated in g / L: peptone 10, yeast extract 6, NaCl 10, xylose 4, and the rest is water, adjust the pH value to 6.4, and autoclave at 115°C for 20 minutes;

[0050] (3) Liquid fermentation culture: inoculate the inoculum of the activated seed solution obtained through step (2) with a volume percentage o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a Paenibacillus xylanilyticus strain and a method for preparing xyloglucanase by using the same. Paenibacillus xylanilyticus B20 CCTCC M 2012194 is used as a fermentation strain to carry out liquid fermentation so as to prepare the xyloglucanase. The preparation method comprises the following steps: activating the strain on a slant culture medium, carrying out seed culture in the liquid seed culture medium, and carrying out liquid state fermentation in the fermentation culture medium to finally obtain the fermentation liquid containing xyloglucanase. Since the Paenibacillus xylanilyticus B20 CCTCC M 2012194 is utilized to prepare the xyloglucanase by fermentation, the invention has the advantages of short fermentation time and high enzyme yield, the production conditions are easy to control, and the products are easy to separate and purify.

Description

technical field [0001] The invention relates to the technical field of microorganisms and enzyme production thereof, in particular to a strain of Paenibacillus xylanolyticum and an enzyme production method thereof. Background technique [0002] Xyloglucanase (Xyloglucanase, XEG) (No. EC3.2.1.151) is a type of xyloglucan-specific endonuclease that hydrolyzes the β-1,4-glycosidic bond in the main chain of xyloglucan. An extracellular enzyme found in the GH5, GH12, GH16, GH44 and GH74 families. Xyloglucanase belongs to a small sub-enzyme in the cellulase system, and has specific degradation ability to polysaccharides with β-1,4-glycosidic bond as the main chain structure, especially xyloglucan, so Xyloglucan can be degraded to produce oligosaccharides. Xyloglucanases, especially xyloglucanases derived from bacteria, generally have good thermal stability and high activity under alkaline conditions, so they can be widely used in various industries. ①In plant production, xylogl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N9/42C12R1/01
Inventor 张梁石贵阳王宝顺丁重阳顾正华
Owner 无锡泰仑达化机设备有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap