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Clean and effective preparation of oligoxylose

A xylooligosaccharide, clean technology, applied in the direction of oligosaccharides, fermentation, etc., can solve the problems of sewage, low efficiency, and many chemical procedures, and achieve the effect of strong production capacity and high efficiency

Inactive Publication Date: 2005-05-11
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The problems in this technology are that the enzymatic hydrolysis reaction solution has a large volume, low concentration, and many impurities, so the post-treatment efficiency is low, there are many chemical procedures, and the requirements for equipment are high, so the production cost remains high.
It can be seen that this is a cumbersome, costly, and sewage-generating process
[0014] Although refined xylan is used as the substrate of the enzymatic hydrolysis reaction, the separation technology of the resulting xylo-oligosaccharides also fails to avoid some inefficient processes such as ultrafiltration, activated carbon or ion resin adsorption / elution, etc.

Method used

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  • Clean and effective preparation of oligoxylose
  • Clean and effective preparation of oligoxylose
  • Clean and effective preparation of oligoxylose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Shake the non-mildew straw to remove dust and dry it and grind it into 1-20mm long shredded grass;

[0042] (2) Weigh 10 kg of grass silk, add 2.5% KOH solution 501, cook for 15 minutes after soaking for 4 hours, and extrude the solution; add 2.5% KOH solution 201 to the grass dregs, cook for 15 minutes again, and extrude solution. Combine the two parts of the extrudate, and centrifuge with a continuous flow centrifuge to obtain about 50 l of the supernatant;

[0043] (3) Add 150L of 95% ethanol, adjust the pH value to 6.0 with phosphoric acid after mixing, and collect the precipitated crude xylan by centrifugation;

[0044] (4) Add 95% ethanol 501 to the coarsely extracted xylan precipitate, break it and stir at low speed for 2 hours to fully balance it, and collect the precipitate by centrifugation; add ethanol and repeat the aforementioned process twice to obtain purified xylan sugar, (see image 3 )HPLC analysis column: Sugarpak1; 6.5mm×300mm sugar column ana...

Embodiment 2

[0048] Embodiment 2: It is basically the same as Embodiment 1, except that the xylan was purchased from Sigma Company of the United States, and the product number is x0627. Dissolve xylan in citric acid buffer to prepare a xylan solution with a concentration of 30%.

Embodiment 3

[0049] Embodiment 3: substantially the same as embodiment 1, the difference is that xylan is prepared by the following method:

[0050] Extract xylan from corncobs with 2% NaOH solution; add 3 times the volume of ethanol with a concentration of about 95% to the alkali extract to precipitate xylan, and centrifuge to separate the precipitate; Dissolve in water, add 3 times the volume of ethanol to precipitate xylan, and collect the precipitate by centrifugation; repeat twice to obtain purified xylan. Dissolve xylan in citric acid buffer to prepare a 20% xylan solution.

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Abstract

A clean an effective preparation of oligoxylose is carried out by: making xylan solution bydissolving xylan in water or buffering liquid, adding xylanase into it to obtain an enzyme hydrolystic liquid, adding alcohol, centrifuging to obtain supernatant, separating to obtain oligoxylose. It costs low, has no pollution, and is effective. Its product likes natural one.

Description

technical field [0001] The invention relates to a preparation method of functional oligosaccharides, in particular to an enzymatic preparation method of xylooligosaccharides, more specifically to a clean and efficient preparation method of xylooligosaccharides. Background technique [0002] Xylooligosaccharides, also known as xylooligosaccharides, are functional oligosaccharides formed by connecting 2-7 xyloses with β-1,4-glycosidic bonds. Compared with other oligosaccharides, xylooligosaccharides have better stability and can specifically promote the proliferation of bifidobacteria. Therefore, it is the most effective bifidus factor, and each person only needs to take 0.7 grams per day. It can achieve the effect of regulating the intestines (generally, the daily dosage of active oligosaccharides is 2-10 grams). [0003] The preparation of xylooligosaccharides usually includes the following three main processes: [0004] 1. Extraction of xylan: Xylan can be extracted from ...

Claims

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Application Information

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IPC IPC(8): C07H3/06C08B37/14C12P19/04
Inventor 邵蔚蓝董宏平薛业敏
Owner NANJING NORMAL UNIVERSITY
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