Glucanases, Nucleic Acids Encoding Them and Methods for Making and Using Them

a technology of glucans and nucleic acids, applied in the field of enzyms, can solve the problems of wort filterability and haze formation, glucans can create sticky dough that is difficult to machine, and reduce biscuit size, so as to achieve the effect of decreasing the viscosity of the beverag
US20110117067A1Inactive Publication Date: 2011-05-19BP CORP NORTH AMERICA INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
BP CORP NORTH AMERICA INC
Publication Date
2011-05-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability, including thermotolerance or thermostability, at increased or decreased pHs and temperatures.
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Description

FIELD OF THE INVENTION

[0001] This invention relates generally to enzymes used in food and feed compositions; and in alternative aspects provides novel enzymes, polynucleotides encoding these enzymes, and uses of these polynucleotides and polypeptides, and in alternative aspects provides polypeptides (e.g., enzymes, peptides, antibodies) having a glucanase activity, e.g., an endoglucanase, activity, e.g., catalyzing hydrolysis of internal endo-β-1,4- and / or β-1,3-glucanase linkages. In one aspect, the endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) comprises hydrolysis of 1,4- and / or β-1,3-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose), lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant or organic material containing cellulosic parts. In one aspect, the polypeptides of the invention have a glucanase, xylanase and / ...

Claims

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