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51 results about "Expansin" patented technology

Expansin refers to a family of closely related nonenzymatic proteins found in the plant cell wall, with important roles in plant cell growth, fruit softening, abscission, emergence of root hairs, pollen tube invasion of the stigma and style, meristem function, and other developmental processes where cell wall loosening occurs. Expansins were originally discovered as mediators of acid growth, which refers to the widespread characteristic of growing plant cell walls to expand faster at low (acidic) pH than at neutral pH. Expansins are thus linked to auxin action. They are also linked to cell enlargement and cell wall changes induced by other plant hormones such as gibberellin, cytokinin, ethylene and brassinosteroids.

Enhancement of accessibility of cellulose by expansins

Plant cell expansion is regulated by wall relaxation and yielding, which is thought to be catalyzed by elusive "wall loosening" enzymes. By employing a reconstitution approach, we initially found that a crude protein extract from the cell walls of growing cucumber seedlings possessed the ability to induce the extension of isolated cell walls. This activity was restricted to the growing region of the stem and could induce the extension of isolated cell walls from various dicots and monocots, but was less effective on grass coleoptile walls. Sequential HPLC fractionation of the active wall extract revealed two proteins with molecular masses of 29 and 30 kD, as measured by SDS-PAGE, associated with such activity. Each protein, by itself, could induce wall extension without detectable hydrolytic breakdown of the wall. We proposed the name "expansins" for this class of proteins. Expansins have been isolated from various plant sources including oat, cucumber, broccoli, celery, tomato, cotton, cabbage, and corn, and also from snail and its feces. These proteins weaken the intermolecular bonds between plant wall polysaccharides. They decrease the mechanical strength of commercial products made from polysaccharides, such as paper, and therefore present a novel approach in developing new technologies in industries which make use of such polysaccharides, such as in the paper industry, in the applications of polysaccharide gums and related products. These proteins moreover present a novel approach in the control of plant growth.
Owner:PENN STATE RES FOUND

Liquid fermentation method for improving yield of cordyceps sinensis polysaccharide by utilizing expansin

The invention relates to a liquid fermentation method for improving yield of cordyceps sinensis polysaccharide by utilizing expansin. The liquid fermentation method includes inoculating cordyceps militaris strains to a PD liquid fermentation medium for activated culture, inoculating the strains to a strain fermentation medium with 10-15% inoculum amount for strain culture to obtain a strain liquid; inoculating the strain liquid to the liquid fermentation medium for liquid culture, adding an expensin solution for continuous culture, separating the liquid to obtain cordyceps militaris mycelium and a fermentation broth; extracting cordyceps militaris extracellular polysaccharide from the fermentation broth, extracting cordyceps militaris intracellular polysaccharide from the cordyceps militaris mycelium, and mixing the cordyceps militaris extracellular polysaccharide and the cordyceps militaris intracellular polysaccharide to obtain the cordyceps sinensis polysaccharide. According to the liquid fermentation method for improving the yield of the cordyceps sinensis polysaccharide by utilizing the expansin, the expansin is applied to liquid fermentation production of the cordyceps militaris polysaccharide, compared with the prior art, yields of the intracellular polysaccharide and the extracellular polysaccharide are improved by more than 1.5 times and 6.9 times respectively, and the industrial application prospect is bright.
Owner:INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI

Special organic fertilizer for konjak

The invention relates to the technical field of konjak planting and discloses a special organic fertilizer for konjak. The special organic fertilizer comprises the following raw materials in parts byweight: 150-180 parts of pig manure, 40-60 parts of cow dung, 30-35 parts of konjak leaves, 10-20 parts of ammonium bicarbonate, 30-50 parts of a composite fertilizer, 10-15 parts of a boron-zinc-magnesium fertilizer, 1-2 parts of composting bacteria, 1-2 parts of crude fiber degrading bacteria, 5-10 parts of potassium sulfate and 5-8 parts of expansin. A method for producing the special organic fertilizer for konjak by using the formula specifically comprises the following steps: crushing the konjak leaves, mixing the konjak leaves with the pig manure and the cow dung according to a weight part ratio, adding the composting bacteria of a same ratio, putting the mixture into a tank for fermentation by using a forklift, carrying out fermentation for 15-20 days, and carrying out turning, wherein the composting fermentation temperature is 45-65 DEG C. The special organic fertilizer for konjak is simple in production procedure, reasonable in cost, considerable in konjak benefit and capableof effectively preventing and treating diseases and insects at a rapid expanding phase of konjak, increasing the harvesting yield of konjak, reducing resource waste and accelerating growth of konjak seedlings at a seedling growth phase as well.
Owner:云南谷力果力农业科技有限公司

Method for preparation of plant suspension culture small cell line by interfering expansin gene Exp2

Belonging to the field of biotechnologies, the invention relates to a method for preparation of a plant suspension culture small cell line by interfering an expansin gene Exp2. The method consists of: first, selecting forward fragments I and II from two conserved regions of gene Exp2 respectively, as well as corresponding reverse sequences I' and II'; constructing RNAi fragments of I-AC-II'-II-CT-I'; and screening out the agrobacterium tumefaciens engineering bacterium inserted in a pCAMBIA2300 plant expression vector; then taking licorice for example, performing agrobacterium-mediated transformation to obtain a transformed callus and its stably expressed suspension cell in order; and then, carrying out nursing culture, and making use of the suspension cell to screen a single cell line callus characterized by fast growth speed and good effect, and a suspension cell line formed thereby; and finally, conducting culture process optimization to obtain the optimum fermentation process parameters of suspension cell growth and product accumulation. The method provided in the invention can obtain the cell line with the advantages of large density, short culture cycle, low cost as well as high production efficiency, and can provide excellent provenances for large-scale development and production of medicinal plant effective components.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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