11 results about "Expansin" patented technology

The invention relates to a method for improving the secretory expression level of expansin in bacillus subtilis, and aims to provide a method capable of promoting the bacillus subtilis to secrete expansin (microorganism-derived expansin BsEXLX1 and plant-derived expansin LeEXP2). The method comprises the following steps: firstly, screening a promoter capable of efficiently starting expansin to construct an engineering strain, and then performing overexpression of a secretory cofactor in the strain to improve the limitation of target protein in the aspects of secretory transport, folding maturation, stability and the like, thereby improving the extracellular secretion level of the two expansin. Amplification verification is carried out through a 5L fermentation tank, the secretion level of expansin is further improved, the expression quantity of BsEXLX1 protein is improved to 1.3 g / L, and the secretion quantity of LeEXP2 is improved to 43.7 mg / L. And a process basis and industrialization potential are provided for subsequent large-scale fermentation production and application development.

The application discloses a biological method for improving the cadmium stress tolerance of sorghum, identifies and clones a cadmium-induced expansin gene SbEXPA11 from a first sorghum variety plant, and transforms the overexpressed SbEXPA11 into a second sorghum variety plant to obtain a third sorghum variety with the gene, which has the biological property of cadmium stress tolerance. The SbEXPA11 overexpression in the sorghum enhances the cadmium tolerance of the sorghum and promotes the long-distance transportation of cadmium from the sorghum root to the stem.

The invention relates to the technical field of biomass energy utilization, and discloses a preparation method of recombinant expansin and application of the recombinant expansin in cellulose enzymolysis sugar production. The application method comprises the following steps: firstly, adding the recombinant expansion protein into cellulose for pretreatment, and then adding cellulase for enzymolysis, so as to improve the yield of reducing sugar in cellulose enzymolysis. The expansin ZM03G10750 has the advantages that the expansin ZM03G10750 is used as a substrate, microcrystalline cellulose is used as the substrate and is subjected to synchronous synergistic enzymolysis with 5 U / g cellulase for 24 hours, the yield of reducing sugar is 235 mg / g and is increased by 21.5%, and the expansin ZM03G10750 and the cellulase have a synergistic effect, namely the expansin ZM03G10750 has a remarkable effect of assisting the cellulase in increasing the enzymolysis rate of cellulose.

The invention discloses a difunctional expansin protein mutant as well as a coding gene and application thereof, and belongs to the technical field of biological genetic engineering. According to the invention, a protein PFLoos1 with bifunctional activity is obtained from a metagenome of rumen microorganisms, and mutants PFLoos1-S1, PFLoos1-S2 and PFLoos1-S3 are obtained by carrying out site-specific mutagenesis on key amino acids in a substrate binding pocket or a surface hydrophobic region of the protein PFLoos1. The mutant provided by the invention has the function of inhibiting the metabolic activity of rumen methanogenic archaea on the basis of keeping the lignocellulose degradation promoting capability. The mutant provided by the invention not only can be applied to preparation of an enzyme preparation for promoting cellulose degradation, but also can be applied to a feed additive for inhibiting ruminant rumen methane generation, and has important practical value for improving feed energy utilization efficiency and low-carbon breeding of animal husbandry.

The invention discloses a rumen anaerobic fungus-derived expansin protein mutant as well as a coding gene and application thereof, and belongs to the technical field of biological genetic engineering. Mutants, namely mutants PFSWO21-N1, PFSWO21-N2 and PFSWO21-N3, which are higher in expression quantity, better in thermal stability and stronger in synergistic effect with cellulase are obtained by performing site-specific mutagenesis on wild type expansin protein PFSWO21, the mutants are successfully expressed in pichia pastoris, and by destroying a hydrogen bond network among cellulose molecules, the expression quantity of the pichia pastoris can be effectively increased, so that the expression quantity of the pichia pastoris can be effectively increased, and the expression quantity of the pichia pastoris can be effectively increased. The mutant can obviously enhance the enzymolysis efficiency of cellulase on lignocellulose, can improve the feed utilization rate of agricultural wastes such as straws and the like, and also can inhibit methane generation by regulating fermentation parameters and microbial community structures in the rumen in-vitro fermentation process, so that the fermentation efficiency is improved. The method has important application value in the aspect of reducing greenhouse gas emission in animal husbandry.

The invention provides an application of a dahurian angelica root expansion protein gene AdEXPA7 in regulation and control of root development. According to the application disclosed by the invention, the fact that the AdEXPA7 has a positive regulation effect on the elongation of root cells is determined by utilizing a biotechnology. Expression of an auxin response gene AdARFs can be induced by applying exogenous auxin to treat an angelica dahurica strain, and the auxin response gene AdARF8-2 is combined with an AuxRE cis-element on a promoter of an expansion protein gene AdEXPA7 of the angelica dahurica, so that expression of the AdEXPA7 is positively regulated and controlled. The root system, hypocotyl and root cell length of the arabidopsis thaliana obtained by over-expression of AdEXPA7 are significantly increased. Therefore, through overexpression of AdEXPA7, the invention has important breeding utilization value for promoting growth and development of arabidopsis thaliana roots.

The present application relates to the field of biotechnology, in particular to a high-yield high-performance cellulase Trichoderma reesei engineering strain and a construction method and application thereof, the Trichoderma reesei engineering strain co-overexpresses beta-glucosidase gene Aabgl1 and expansin gene Trswo1, and knocks out transcriptional repressor gene Trace1, the Trichoderma reesei engineering strain has high cellulase yield, which is 100.15% higher than that of the starting strain Trichoderma reesei Rut C30.The high-performance cellulase produced by the Trichoderma reesei engineering strain has balanced enzyme system composition and excellent hydrolysis performance, when corn straw is hydrolyzed, the glucan conversion rate reaches 94.89%, the hydrolysis performance is equivalent to that of commercial enzyme preparation Cellic CTec2.0, and good industrial development prospect and practical application value are shown.

This invention relates to the field of genetic engineering technology, specifically providing a soybean extended protein gene. GmEXPA10 and its applications, the GmEXPA10 The dominant expression in soybean pods is an open reading frame (ORF) of 747 bp, encoding 248 amino acid residues. Further analysis in this invention revealed that... GmEXPA10 Overexpression significantly increased the root length of transgenic Arabidopsis thaliana, with a greater increase under acidic conditions; GmEXPA10 Overexpression significantly increased the length and width of transgenic Arabidopsis thaliana pods; GmEXPA10 Gibberellin expression in pods was induced. Analysis of different soybean varietal resources... GmEXPA10 Allelic variation revealed that a downstream SNP (C / A) can classify soybean resources into two types, with significant differences in 100-seed weight and seed width between the types, indicating that the SNP site is related to soybean seed size and weight.

The invention belongs to the technical field of genetic engineering, and discloses a tea tree expansin CsEXPA10 gene and application thereof in regulating and controlling cold resistance of tea trees. The gene is a tea tree EXPANSIS family gene, the nucleotide sequence is shown as SEQ ID NO. 1, and the sequence of an encoded protein is shown as SEQ ID NO. 2. The gene is in a down-regulation expression mode at the low temperature of 4 DEG C and is a negative regulation factor of low-temperature response of the tea tree; overexpression of the gene can aggravate low-temperature damage of tea tree leaves, and low-temperature damage of tea tree shoots can be remarkably relieved by inhibiting expression of the gene through an antisense oligonucleotide technology. The invention further constructs recombinant plasmids and transgenic engineering bacteria containing the gene, the function of the gene in low-temperature stress response of the tea tree is defined for the first time, the research blank of the expansion protein in the field of cold-resistant regulation of the tea tree is filled, and a new thought is provided for research of a cold-resistant mechanism of the tea tree; and a gene target and a technical support are provided for genetic improvement of the cold resistance character of the tea tree.

The invention discloses an expansin mutant with a function of inhibiting methane bacteria as well as a coding gene and application of the expansin mutant, and belongs to the technical field of biological genetic engineering. The invention provides an expansin mutant which comprises a mutant PS1-M1 and a mutant PS1-M2, the mutant PS1-M2 is obtained by mutating 173rd-site serine of PFSWO11 expansin-like protein into alanine and mutating 176th-site glutamine into phenylalanine, hydrophobic amino acid sites in the protein are replaced, so that the hydrophobicity of the protein is reduced or spatial rearrangement occurs, and the expansin-like protein is obtained. On the basis of maintaining the structural stability, the mutant PSI-M1 and the mutant PSI-M2 have the ability of inhibiting the growth or metabolic activity of rumen methanogenic archaea, so that the rumen methane generation is reduced, the feed energy utilization efficiency is improved, a new biological technical approach is provided for ruminant methane emission reduction, and the mutant PSI-M1 and the mutant PSI-M2 have popularization significance.

The application discloses a mutant of expansin protein from rumen anaerobic fungi, a coding gene and application thereof, and belongs to the technical field of biological genetic engineering. The wild-type expansin protein PFSWO2_1 is subjected to site-directed mutation, and mutants with higher expression, better thermal stability and stronger synergistic effect with cellulase, namely the mutants PFSWO2_1-N1, PFSWO2_1-N2 and PFSWO2_1-N3, are obtained. The mutants are successfully expressed in Pichia pastoris, the hydrogen bond network between cellulose molecules is destroyed, the mutants can significantly enhance the enzymolysis efficiency of cellulase on lignocellulose, the mutants can not only improve the utilization rate of agricultural wastes such as straws in feed, but also can inhibit the generation of methane by regulating the fermentation parameters and microbial community structure in the rumen in vitro fermentation process, and the mutants have important application value in reducing the greenhouse gas emission of the animal husbandry.