40results about How to "Increase the number of nodules" patented technology

The invention belongs to a method for producing plant fertilizers, more particularly to a method for producing a solid-state Ca / Mg / Ti / Fe / S / N / Si compound fertilizer from water-quenched blast furnace slag. The invention is characterized in that: the raw materials include titanium oxide-containing water-quenched blast furnace slag, ammonium sulfate, ammonium hydrogen sulfate, ammonium pyrosulfate and ammonium persulfate; the method comprises the following steps: ball-milling Ti-containing water-quenched blast furnace slag to obtain powder of particle size of 60-160 micrometers, mixing the furnace slag powder with 3-20 times of one substance selected from ammonium sulfate, ammonium hydrogen sulfate, ammonium pyrosulfate, ammonium persulfate, or a mixture thereof, heating the resulting mixture up to 200-500 DEG C and maintaining the temperature for 10-65 min, and crushing clinkers to small pieces of 4-12 mm diameter. The invention utilizes Ti-containing water-quenched blast furnace slag and other raw materials to produce plant fertilizers, and thereby realizes environmental protection, Ti resource utilization and nutrient supplementation to plants.

The invention discloses a gene GmSPX5 for regulating and controlling growth of leguminosae root nodules, and the application of gene GmSPX5. The nucleotide sequence of the gene is as shown in SEQ ID NO.1 and the coding amino acid sequence is as shown in SEQ ID NO.2. The research shows that excessive expression of the GmSPX5 can increase the number of the leguminosae root nodules and the density ofroot nodules infecting cells under high phosphorus treatment; furthermore, the nitrogen phosphorus content and the yield of soybeans can be remarkably increased. Therefore, the GmSPX5 can regulae andcontrol the growth and development of the root nodules and has important effects of improving the nitrogen phosphorus nitration of plants and increasing the yield; and the gene GmSPX5 can be used forregulating and controlling the adaptability on phosphorus deficiency and nitrogen deficiency in soil by the plants through a transgenic technology, also can be applied to leguminous crop nitrogen phosphorus synergistic and efficient genetic improvement and has a very important market prospect.

The invention relates to the field of biological processing, and in particular relates to a processing method of kitchen wastes and the application of a processed product as an organic fertilizer. The processing method is simple, and the kitchen wastes are effectively utilized. A kitchen waste processed product is rich in nutrients and can be used for preparing the organic fertilizer. Moreover, the invention also provides the organic fertilizer. The organic fertilizer comprises the kitchen waste processed product and acceptable elements in the fertilizer. By applying the organic fertilizer containing the kitchen waste processed product, the number of root nodules of summer soybeans can be effectively increased, the chlorophyll content, leaf nitrogen content, net photosynthetic rate and yield of the summer soybeans are improved, and increase of the farmer income is promoted.

This invention provides anti-weight yin cha advancing crop yield increasing special fertilizer. The fertilizer has nitrogen in plant nutrition elements, phosphor P205, kalium K20, middle microelement chelated additive T, their weight parts are as follow, P2O5:K2O:T equals to 1:2:0-5.0:0.429>=T>0.0, it also has titanium-ascorbic acid solid compound used as plant grow regulator, the compound adding on is 0.005-0.5 percent to the fertilizer weight. In this invention, absorptivity is high, sublimation activity of enzyme inside plant body can be greatly improved, plant chlorophyll forming and nitrogen, phosphor and kalium absorbing can also be improved, plant root system activity and growth can be improved, crop photosynthesis can be improved and crop anti inverse ability and damage by disease and insect can also be improved. This invention can increase root nodule number, increase plant nitrogen-fixing capacity and resistibility to microorganism, frequency of processing plant can be reduced, it also has good production increasing function to crop growth.

A biological source activator for enhancing nitrogen fixation ability of native rhizobia is disclosed, which is prepared by method of: (1) slant culturing T216 mother fungi of trichoderma harziarum strain; (2) fermenting in fermentation jar; (3) adjusting pH to 7-8 with sodium hydroxide solution after fermentation, sealing the jar for one night, vacuum filtering with sterilized gauze, obtaining filtrate, sealed packaging. Before seeding of legume crops, the inventive biological source activator is added into seed soaking liquid in accordance of 20-30% of volume ratio and seed is soaked for 4-6 hours, or when seedlings of legume crops are cultured, the biological source activator is diluted 4-5 times and root is soaked for half a hour, thus when crops are growing, root nodulation of native rhizobia is induced, and nitrogen fixation ability is evidently increased.

The invention belongs to a method for producing plant fertilizers, more particularly to a method for producing a solid-state Ca / Mg / Ti / Fe / S / N / Si compound fertilizer from water-quenched blast furnace slag. The invention is characterized in that: the raw materials include titanium oxide-containing water-quenched blast furnace slag, ammonium sulfate, ammonium hydrogen sulfate, ammonium pyrosulfate and ammonium persulfate; the method comprises the following steps: ball-milling Ti-containing water-quenched blast furnace slag to obtain powder of particle size of 60-160 micrometers, mixing the furnace slag powder with 3-20 times of one substance selected from ammonium sulfate, ammonium hydrogen sulfate, ammonium pyrosulfate, ammonium persulfate, or a mixture thereof, heating the resulting mixtureup to 200-500 DEG C and maintaining the temperature for 10-65 min, and crushing clinkers to small pieces of 4-12 mm diameter. The invention utilizes Ti-containing water-quenched blast furnace slag and other raw materials to produce plant fertilizers, and thereby realizes environmental protection, Ti resource utilization and nutrient supplementation to plants.

The invention discloses stress resistance and nitrogen fixation bradyrhizobium sp. suitable for the northeast area and an application thereof. The invention firstly discloses bradyrhizobium sp. DBPB1;the bradyrhizobium sp. DBPB1 has the preservation number of CGMCC No.17502 in China General Microbiological Culture Collection Center. The invention further provides a bacterial agent including the bradyrhizobium sp. and an application thereof. The bradyrhizobium sp. DBPB1 has the characteristics of promoting growth, has high adaptability, is a biosafe strain, can significantly increase the plantheight, the number of root nodules, the fresh weight on the ground and other traits of peanut in different development periods, greatly improves the yield of peanut, and provides the northeast area,one of main peanut planting areas in China, with microbial germplasm resources with high efficient nitrogen fixation and stable growth promotion, and is conducive to promoting green, healthy and sustainable development of agriculture in China.

The invention relates to the field of biological processing and particularly relates to a processing method of kitchen wastes and an application of a kitchen waste processed product as an organic fertilizer. The processing method is simple and capable of effectively utilizing kitchen wastes. A prepared kitchen waste processed product is rich in nutrient and can be used for preparing the organic fertilizer. In addition, the invention provides an organic fertilizer comprising the kitchen waste processed product and elements which can be accepted in the fertilizer. By applying the organic fertilizer containing the kitchen waste processed product, the root nodule number of summer soybeans can be effectively increased, the chlorophyll content, leaf blade nitrogen content and net photosynthetic rate of the summer soybeans can be increased, the yield of the summer soybeans can be increased, and the farmer income can be increased.

The invention relates to a construction method of genetic engineering strains for improving quantity of soybean nodules. The construction method aims at solving the problems that when the conventional rhizobium japonicum genetic engineering strains are inoculated to soybean seedlings, the root nodule quantity of soybean plants and the yield increase of soybeans are small compared with those of the original starting strains. The construction method comprises the following steps of: performing activation and culture expansion on sinorhizobium fredii to extract genomic DNA (deoxyribonucleic acid); performing PCR (Polymerase Chain Reaction) amplification by virtue of a homologous sequence cloning method to obtain target gene dct A; performing sequencing identification on the target gene; constructing a prokaryotes expression vector pTR-Plac-dct A; and converting the prokaryotes expression vector into the sinorhizobium fredii to obtain the gene engineering strains. The quantity of the root nodules on the soybean plant with the transformed gene engineering strains is increased by 53.73%, and the yield of the soybeans is increased by 62.02%.

The invention relates to a special fertilizer for culturing Chinese clover. The special fertilizer for culturing Chinese clover comprises the following raw materials by weight: 80-110 Kg of urea, 40-60 Kg of ammonium sulfate, 410-450 Kg of ammonium diacid phosphate, 250-275 Kg of potassium chloride, 25-35 Kg of borax, 1 Kg of ammonium molybdate, 60 Kg of zeolite powder, 61-79 Kg of attapulgite clay powder; the raw materials are mixed uniformly, crushed, granulated and dried to obtain the special particle fertilizer for Chinese clover; the zeolite powder adopts agricultural zeolite of 100 meshes above; and the special particle fertilizer for Chinese clover is screened sequentially by a screen with a diameter of 4.75mm and a screen with a diameter of 1.00mm, and then is coated to obtain the special fertilizer for culturing Chinese clover. After the special fertilizer for culturing Chinese clover is applied to Chinese clover, Chinese clover grows vigorously and healthily and the root nodules are increased, and the fresh grass yield is increased by more than 25% per acre.

The invention relates to a special fertilizer for culturing Chinese clover. The special fertilizer for culturing Chinese clover comprises the following raw materials by weight: 80-110 Kg of urea, 40-60 Kg of ammonium sulfate, 410-450 Kg of ammonium diacid phosphate, 250-275 Kg of potassium chloride, 25-35 Kg of borax, 1 Kg of ammonium molybdate, 60 Kg of zeolite powder, 61-79 Kg of attapulgite clay powder; the raw materials are mixed uniformly, crushed, granulated and dried to obtain the special particle fertilizer for Chinese clover; the zeolite powder adopts agricultural zeolite of 100 meshes above; and the special particle fertilizer for Chinese clover is screened sequentially by a screen with a diameter of 4.75mm and a screen with a diameter of 1.00mm, and then is coated to obtain the special fertilizer for culturing Chinese clover. After the special fertilizer for culturing Chinese clover is applied to Chinese clover, Chinese clover grows vigorously and healthily and the root nodules are increased, and the fresh grass yield is increased by more than 25% per acre.

The invention discloses an application of a gene GmMDH12 in promotion of nodulation and nitrogen fixation capacity of soybeans, and particularly relates to an application of a malate dehydrogenase gene GmMDH12 in control for synthesis of malic acid of root nodules and in promotion of nodulation of leguminous crop root system. The research indicates that GmMDH12 has a function of regulating and controlling synthesis of malic acid of the soybean root nodules, concentration of endogenous malic acid of the root nodules of transgenic composite soybean plants is remarkably increased by over-expression of GmMDH12, the number and the biomass of the root nodules are increased, and accordingly, nitrogen content and biomass of the plants are increased. According to the invention, the gene GmMDH12 haspotential of improving biological nitrogen fixation function of the leguminous crops with a transgenic technology and has great theoretical and practical significance for efficient utilization of crop nutrients and sustainable development of environment-friendly agriculture.

The invention discloses a peanut seedling stage control and supplementary integrated composite preparation and a using method thereof. The composite preparation is prepared from, by weight, 30-40 parts of wettable powder containing 5% of uniconazole, 15-20 parts of water dispersible granules containing 10% of difenoconazole, 15-20 parts of ammonium molybdate and 20-30 parts of boric acid. According to the composite preparation, the effective component uniconazole can effectively control the basal internode length and the plant height of peanuts, the peanut plant height is decreased by 20.0%-28.3%, basal internodes are shortened by 25.9%-43.0%, uniconazole also can control damage of a low-light environment to the chloroplast structure of peanut leaves, and the leaf spad value is increased by 12.3%-15.4%; difenoconazole can prevent and control occurrence of peanut diseases; by applying molybdenum fertilizer in the seedling stage, growth of root nodules can be promoted, and the root nodule number is increased by 18.3%-39.4%; boric fertilizer can promote flower bud differentiation and flowering and needle penetrating, and the fruit needle number is increased by 12.5%-34.1%.

The invention discloses a tillage-free high-yield technology for soybeans without application of chemical fertilizer and belongs to the technical field of soybean planting. The problems that the chemical fertilizer is generally used for the soybeans in Heilongjiang and other places of China, tilling is conducted many times, the production and investment costs are high, the yield and the efficiencyare low, the soil ecological environment is gradually deteriorated and the product quality declines are solved. The technology is achieved by selecting non-transgenic high-nitrogen-fixation and high-yield seeds of the soybeans, conducting planting in large ridges with the width of 130 cm, conducting sparse planting and conducting rational crop rotation without application of the chemical fertilizer and tillage. The technology is simple in operation, a method is simple, and the technology is easy to apply and popularize.

The invention discloses new application of a florigen gene GmFT2a to improvement of biological nitrogen fixation efficiency of leguminous plants. The florigen gene GmFT2a is mainly expressed in laterdevelopment period of root nodules and is positioned in cell nucleus and cytoplasm of neoplastic cortical cells, so that the number and weight of the soybean root nodule are significantly increased, the activity of dinitrogenase is significantly improved, the nitrogen content and the phosphorus content of plants are increased, and the biomass of transgenic plants is finally increased. Efficient utilization of nitrogen and phosphorus nutrients of leguminous crops can be realized, gene resources are provided for high-yield molecular breeding as well as genetic improvement of photoperiodicity, and important guidance and practical significance in developing fertilize-reducing and effect-improving sustainable ecological agriculture are achieved.

The invention discloses new application of a cell wall expansin gene GmEXPB6, and particularly discloses new application of the GmEXPB6 gene on the aspects of promoting the biological nitrogen fixation of a leguminous plant and improving the nitrogen fixation efficiency. The inventors clone the gene GmEXPB6 with low-phosphorous enhancement and root nodule high expression through a quantitative PCR result and prove that the protein coded by the GmEXPB6 gene has the functions of promoting root nodule expansion, enhancing the root nodule nitrogenase activity and the like, and the root nodule nitrogen fixation efficiency and biomass of a transgenic compound plant are finally improved through GmEXPB6 overexpression. The study of the inventors can provide the gene resource for the nitrogen high-efficiency and high-yield molecule breeding of crops which include soybeans, and has important theoretical and practical significance on developing environmental-friendly sustainable agriculture.

The invention discloses novel application of florigen gene GmFT5a in promoting biological nitrogen fixation for leguminous plants and improving nitrogen and phosphorus nutrient efficiency. The florigen gene GmFT5a starts expressing upon rapid development of root nodules and locating at nuclei and cytoplasm of cortical cells of the root nodules, thereby significantly increasing the quantity and weight of soybean root nodules and dinitrogenase activity under both long and short durations of sunshine, increasing nitrogen and phosphorus contents in plants, and finally increasing biomass quantity of transgenic plants. Nitrogen and phosphorus nutrition in leguminous crops can be efficiently utilized; candidate gene resources are provided for high-yield molecular breeding with good photoperiod eurytopicity; the florigen gene GmFT5a has significant theoretical and practical significances in the development of environment-friendly sustainable ecological agriculture.

The invention discloses a humic acid fertilizer synergist with an ammonia volatilization inhibition effect and a preparation method thereof. The synergist is prepared from the following raw materials in parts by weight: 65-75 parts of humic acid soil, 3-6 parts of a strain, 12-14 parts of a carbon source, 6-8 parts of a nitrogen source, 35-45 parts of water, 2-3 parts of medium trace elements, 0.08-0.12 part of ferrous carbonate, 0.6-0.8 part of a film forming agent and 1.5-3 parts of an organic acid regulator. According to the synergist prepared by utilizing a humic acid synergetic solution, all components in the humic acid synergetic solution are purely natural substances, have excellent environmental friendliness, can be completely decomposed, and can be used for promoting absorption of nutrition in soil, increasing the yield of crops, improving the quality of agricultural products, reducing nutrition loss, avoiding secondary pollution, reducing agricultural production cost and supplying trace elements in the soil. The humic acid fertilizer synergist has the characteristics of wide raw material source, low cost, simple production process, convenience in use in fertilizer production and the like, and has a wide application prospect in synergizing production.

The invention discloses a method for increasing an amount of plant root nodules on the basis of gene negative regulation and control. The method comprises the following steps of: firstly, extracting atotal RNA (Ribonucleic Acid) in a plant tissue; through reverse transcription PCR (Polymerase Chain Reaction), synthesizing a PCR template; constructing a recombinant expression vector used for knocking out a corresponding gene as a knockout carrier; and through an agrobacterium tumefaciens-mediated hairy root transformation method, transforming a receptor plant so as to down-regulate the expression of the corresponding gene in the receptor plant and increase the nodulation number of the root system of the receptor plant. The recombinant expression vector constructed by the method disclosed by the invention is used for transforming the receptor soybean plant to obtain a transgenosis plant, and the number of root nodules of a soybean root system can be obviously improved.

The invention discloses a culture method for a high scaffolding nitrogen fixation plant. In a plant body, separated over-expression or simultaneous over-expression of nucleotide sequences shown in SEQID NO:1-SEQ ID NO:5 in a sequence list is performed, and a transgenic plant with the high scaffolding nitrogen fixation capability is obtained compared with a normal plant. By means of a constructedover-expression vector, a receptor soybean plant is converted to obtain the transgenic plant, the number of soybean root nodules can be remarkably increased, and the method has a great significance inreducing nitrogen fertilizer application and improving the soybean yield.

The invention discloses new application of a florigen gene GmFT2a to improvement of biological nitrogen fixation efficiency of leguminous plants. The florigen gene GmFT2a is mainly expressed in laterdevelopment period of root nodules and is positioned in cell nucleus and cytoplasm of neoplastic cortical cells, so that the number and weight of the soybean root nodule are significantly increased, the activity of dinitrogenase is significantly improved, the nitrogen content and the phosphorus content of plants are increased, and the biomass of transgenic plants is finally increased. Efficient utilization of nitrogen and phosphorus nutrients of leguminous crops can be realized, gene resources are provided for high-yield molecular breeding as well as genetic improvement of photoperiodicity, and important guidance and practical significance in developing fertilize-reducing and effect-improving sustainable ecological agriculture are achieved.

The invention relates to the field of biological processing, and in particular relates to a processing method of kitchen wastes and the application of a processed product as an organic fertilizer. The processing method is simple, and the kitchen wastes are effectively utilized. A kitchen waste processed product is rich in nutrients and can be used for preparing the organic fertilizer. Moreover, the invention also provides the organic fertilizer. The organic fertilizer comprises the kitchen waste processed product and acceptable elements in the fertilizer. By applying the organic fertilizer containing the kitchen waste processed product, the number of root nodules of summer soybeans can be effectively increased, the chlorophyll content, leaf nitrogen content, net photosynthetic rate and yield of the summer soybeans are improved, and increase of the farmer income is promoted.

The invention relates to a low-temperature phosphorus-dissolving Sinorhizobium sp. and its bacterial agent. The strain name is ATHC‑2, and the classification name is Sinorhizobium sp.. The depository unit is the General Microbiology Center of China Microbiological Culture Preservation Management Committee. The number is CGMCC No.10651, the preservation date is: March 20, 2015, and the preservation address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The bacterial strain ATHC-2 of the present invention can withstand the influence of low temperature on its growth, and has strong phosphorus-dissolving activity. The impact of low temperature stress on soybean nodulation and nitrogen fixation in agricultural production can be alleviated by applying low temperature resistant rhizobia agents, improving soil fertility, reducing the use of chemical fertilizers, and improving the yield and quality of soybeans and other crops.

The invention provides application of loading protein gene GmPHF1a of a phosphorus transporter gene and particularly relates to application of gene GmPHF1a in terms of promoting phosphorus acquisitionin leguminous plants to promote nodulation and nitrogen fixing, and nitrogen increasing via phosphorus. The gene GmPHF1a that has expression in all leaves, stems, roots and nodules is cloned throughquantitative PCR (polymerase chain reaction); it is proved that a protein encoded by the gene GmPHF1a can interact with phosphorus transporter protein GmPT5 / GmPT7, which controls phosphorus absorptionand transport in nodules, the nodule quantity and nodule weight can be significantly increased by interference of GmPHF1a, nitrogen content, phosphorus content and biomass quantity are increased, andsoybean yield is significantly increased.

The invention discloses a transgenic plant breeding method for increasing the number of root nodules and promoting the development of root nodules, overexpressing the gene shown in SEQ ID NO: 1 or SEQ ID NO: 2 in the sequence list in the plant or its homologous / equivalent Genes to obtain transgenic plants with increased number of nodules and / or enhanced nodule development compared to normal plants. Using the method of the invention to construct transgenic plants can significantly increase the number of root nodules and promote the maturity of root nodules, and has great practical value in theoretical research and agricultural and forestry applications.

The invention discloses a method for improving the efficiency of plant nodulation and nitrogen fixation. Firstly, construct a recombinant expression vector containing the gene fragment shown in SEQ ID NO: 1, then use the recombinant expression vector to construct a transformant, and then use the transformant to infect the target For plants, positive plants were screened to obtain transgenic plants with increased number of nodules and enhanced nodulation / nitrogen fixation ability compared with normal plants. The transgenic soybean constructed by the method of the invention has significantly increased number of nodules and higher nitrogen fixation efficiency.

The invention discloses GmMDH12 Application of genes in promoting soybean nodulation and nitrogen fixation. malate dehydrogenase gene GmMDH12 Control of malate synthesis in root nodules and its use in promoting root nodulation in leguminous crops. The present invention finds that, GmMDH12 Has the function of regulating malic acid synthesis in soybean nodules, overexpression GmMDH12 Significantly increased the concentration of endogenous malic acid in root nodules of soybean transgenic compound plants, increased the number of nodules and the biomass of nodules, and thus increased the nitrogen content and biomass of plants. According to the present invention, GmMDH12 Genes have the potential to improve the biological nitrogen fixation function of legume crops through transgenic technology, which has important theoretical and practical significance for improving the efficient use of crop nutrients and the development of environmentally friendly and sustainable agriculture.

The invention discloses a method for promoting the growth of clover and / or increasing the yield of clover and the bacterial agent used therein. The method for promoting the growth of clover and / or increasing the yield of clover comprises the step of mixing the seeds of clover with the inoculum before sowing the soil; the inoculation contains the metabolites of Rhizobium clover or / and the Rhizobium clover ; The strain number of the Rhizobium clover bacteria is 92-29, and its preservation number in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No.15533. Clover rhizobia 92-29 of the present invention significantly promotes the growth of clover and significantly improves the output of clover under acidic soil conditions: improve the plant height, nodulation rate, root nodule number and / or root nodule weight of clover; increase the plant fresh weight and dry weight of clover Heavy, increases clover's fresh grass yield and dry grass yield. The invention has broad application prospects in the clover planting industry.

This invention provides anti-weight yin cha advancing crop yield increasing special fertilizer. The fertilizer has nitrogen in plant nutrition elements, phosphor P205, kalium K20, middle microelement chelated additive T, their weight parts are as follow, P2O5:K2O:T equals to 1:2:0-5.0:0.429>=T>0.0, it also has titanium-ascorbic acid solid compound used as plant grow regulator, the compound adding on is 0.005-0.5 percent to the fertilizer weight. Its preparation technique is chemical synthesis oil cooling method. Titanium-ascorbic acid solid compound can improve plant absorbing to microelement, the function of titanium improving photosynthesis and synthesis of carbohydrate can be fully exerted, it can increase root nodule number, increase plant nitrogen-fixing capacity and resistibility to microorganism, it also has good production increasing function to crop growth. In this invention, the fertilizer is in plocoid state under high temperature, and this is good fertilizer efficiency to soil and crops. Granulation is realized by using oil cooling, oil temperature heat emission is slow, particle cooling is uniform, and pollution brought by coal heating can be reduced.