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Coculturing fermentation method of rhizobium and Bacillus phosphorus

A fermentation method and technology of Bacillus megaterium, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc. It has been reported that the number of rhizobia is small, etc., to achieve the effect of increasing production and saving fertilizer, improving the growth of seedlings, and the effect of wide application prospects

Inactive Publication Date: 2007-01-24
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this invention only solves the problem of nitrogen fixation, and the co-culture fermentation method for rhizobium and Bacillus megaterium has not been reported so far
And because rhizobia grows slower than bacillus, if these two kinds of bacteria are mixed and cultivated according to conventional methods, there will be bacillus that grows faster to suppress the growth of rhizobia, which will eventually lead to a large number of bacillus in its fermentation liquid and nodules. The phenomenon of extremely low number of bacteria

Method used

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  • Coculturing fermentation method of rhizobium and Bacillus phosphorus
  • Coculturing fermentation method of rhizobium and Bacillus phosphorus
  • Coculturing fermentation method of rhizobium and Bacillus phosphorus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Bradyrhizobium escherichia AM01 and Bacillus megaterium ACCC11107 co-culture fermentation

[0023] Rhizobium AM01 and Bacillus megaterium ACCC11107 were cultured on the solid plate of strain culture medium respectively. Bacillus ACCC11107 was cultured on the solid plate of the strain medium at 28-30°C for 24 hours. The composition of the medium was: peptone 5g, beef extract 3g, NaCl 5g, agar 15g, distilled water to 1000mL, medium pH 7.0-7.2 . The culture medium was sterilized at a pressure of 103 kPa at 121° C. for 30 minutes. Rhizobia AM01 was cultured on the solid plate of the strain culture medium at 28-30°C for 72 hours. The composition of the culture medium was: mannitol 10g, sodium glutamate 0.5g, K 2 HPO 4 0.5g, MgSO 4 7H 2 O 0.2g, NaCl 0.01g, yeast extract 0.5g, agar 1.5g, dilute to 1000mL with distilled water, medium pH 6.8-7.0. The culture medium was sterilized at a pressure of 103 kPa at 121° C. for 30 minutes.

[0024]Single colonies of...

Embodiment 2

[0026] Example 2: Biological characteristics of the co-cultured fermentation broth of Bradyrhizobium escheri AM01 and Bacillus megaterium ACCC11107

[0027] Get the fermented liquid after the co-cultivation of Example 1 and measure its nitrogen fixation activity with known acetylene reduction method, measure its available phosphorus content with known phosphomolybdenum blue colorimetric method, measure viable bacteria quantity with known plate dilution counting method, additionally use The same method was used to measure the nitrogen fixation activity of the single-strain rhizobium AM01 fermentation broth, the single-strain fermentation broth of Bacillus megaterium ACCC11107, and the 3:1 mixture of AM01 and ACCC11107, the available phosphorus content and the number of viable bacteria for comparison. The results are shown in Table 1.

[0028] Measurement items

Embodiment 3

[0029] Embodiment 3: Liaoning Bradyrhizobium AJ02 and Bacillus megaterium ACCC11107 co-culture fermentation

[0030] Rhizobia AJ02 and Bacillus megaterium ACCC11107 were cultured on the solid plate of strain medium respectively. Bacillus ACCC11107 was cultured on the solid plate of the strain medium at 28-30°C for 24 hours. The composition of the medium was: peptone 5g, beef extract 3g, NaCl 5g, agar 15g, distilled water to 1000mL, medium pH 7.0-7.2 . The culture medium was sterilized at a pressure of 103 kPa at 121° C. for 30 minutes. Rhizobia AJ02 was cultured on the solid plate of the strain medium at 28-30°C for 72 hours, and the composition of the medium was: mannitol 10g, sodium glutamate 0.5g, K 2 HPO 4 0.5g, MgSO 4 7H 2 O 0.2g, NaCl 0.01g, yeast extract 0.5g, agar 1.5g, dilute to 1000mL with distilled water, medium pH 6.8-7.0. The culture medium was sterilized at a pressure of 103 kPa at 121° C. for 30 minutes.

[0031] Single colonies of Rhizobium AJ02 and Bac...

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Abstract

A common culturing-fermenting method of rhizobia and P-decomposing bacillus megaterium for preparing multi-function bacterial fertilizer includes such steps as inoculating rhizobia in the fermentive culture medium, culturing in constant-temp gyratory shaker for 24-36 hr, inoculating said bacillus megaterium in it, and culturing in constant-temp gyratory shaker for 48-60 hr.

Description

technical field [0001] The invention relates to a co-cultivation and fermentation method, in particular to a co-cultivation and fermentation method of rhizobia and Bacillus megaterium capable of dissolving phosphorus. Background technique [0002] Both phosphorus and nitrogen are important nutrients necessary for plant growth and development. Most of the phosphorus in the soil is in the state of organic or inorganic phosphate, which cannot be directly absorbed by plants. Phosphorus that is difficult for plants to absorb and utilize can be converted into phosphorus that can be absorbed and utilized by plants through phosphate-dissolving bacteria. Nitrogen in the air is in a molecular state, which is difficult for plants to absorb. The nitrogen fixation of rhizobia can make plants fully absorb nitrogen in the air. Therefore, the utilization of rhizobia and phosphorus-solubilizing bacteria is of great significance for promoting plant growth. How to combine rhizobia and phospho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/11C12R1/41
Inventor 康丽华马海宾江业根陈应龙
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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