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Tissue culture method for cordia dichotoma

A tissue culture and rag wood technology, applied in the field of plant cultivation, can solve problems such as no records, and achieve the effects of strong growth, rapid differentiation of axillary buds, and convenient transplanting.

Inactive Publication Date: 2018-12-21
王远能
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, ragwood is mainly wild. With the in-depth research, development and utilization of ragwood, it is imperative to develop more artificial cultivation techniques for ragwood. There is no relevant technology for the tissue cultivation of ragwood. see record

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The tissue culture method of ragwood, comprises the following steps:

[0025] (1) Explant treatment: when the ragwood sprouts in spring, collect the intact shoots of the ragwood without diseases and insect pests as explants, wash them with pure water for 3 times, and use a potassium permanganate solution with a mass fraction of 0.35%. Disinfect for 15 minutes, rinse twice with sterile water, air dry naturally, and set aside;

[0026] (2) Callus culture: In a sterile environment, cross-cut shoots with a length of 3 to 5 cm, put the shoots into callus culture medium, and cultivate them at a temperature of 25 to 28 °C and a humidity of 85 to 90 °C. %, cultivated in dark for 25 days to obtain callus;

[0027] The formula of the callus culture medium is: MS medium+0.42mg / L naphthaleneacetic acid+7.8g / L sucrose+1.6 mg / L indolebutyric acid+0.14% active carbon+5.8g / L agar.

[0028] (3) Bud induction culture: under aseptic operation, select the callus that is not contaminated...

Embodiment 2

[0035] The tissue culture method of ragwood, comprises the following steps:

[0036] (1) Explant treatment: when the ragwood sprouts in spring, collect the intact shoots of the ragwood without diseases and insect pests as explants, wash them with pure water for 3 times, and use a potassium permanganate solution with a mass fraction of 0.35%. Disinfect for 15 minutes, rinse twice with sterile water, air dry naturally, and set aside;

[0037] (2) Callus culture: In a sterile environment, cross-cut shoots with a length of 3 to 5 cm, put the shoots into callus culture medium, and cultivate them at a temperature of 25 to 28 °C and a humidity of 85 to 90 °C. %, cultivated in dark for 25 days to obtain callus;

[0038] The formula of the callus culture medium is: MS medium+0.42mg / L naphthaleneacetic acid+7.8g / L sucrose+1.6 mg / L indolebutyric acid+0.14% active carbon+6.0g / L agar.

[0039] (3) Bud induction culture: under aseptic operation, select the callus that is not contaminated...

Embodiment 3

[0046] The tissue culture method of ragwood, comprises the following steps:

[0047] (1) Explant treatment: when the ragwood sprouts in spring, collect the intact shoots of the ragwood without diseases and insect pests as explants, wash them with pure water for 3 times, and use a potassium permanganate solution with a mass fraction of 0.35%. Disinfect for 15 minutes, rinse twice with sterile water, air dry naturally, and set aside;

[0048] (2) Callus culture: In a sterile environment, cross-cut shoots with a length of 3 to 5 cm, put the shoots into callus culture medium, and cultivate them at a temperature of 25 to 28 °C and a humidity of 85 to 90 °C. %, cultivated in dark for 25 days to obtain callus;

[0049] The formula of the callus culture medium is: MS medium+0.42mg / L naphthaleneacetic acid+7.8g / L sucrose+1.6 mg / L indolebutyric acid+0.14% active carbon+6.0g / L agar.

[0050] (3) Bud induction culture: under aseptic operation, select the callus that is not contaminated...

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PUM

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Abstract

The invention discloses a tissue culture method for cordia dichotoma. The tissue culture method comprises the following steps: (1) explant processing; (2) callus culture; (3) bud induction culture; (4) rooting culture; (5) transplantation seedbed culture; (6) seedbed management. The tissue culture method for the cordia dichotoma has the advantages of short culture period, high breeding speed, quick axillary bud differentiation, rapid rooting, developed root systems and the like, the survival rate of the cultured cordia dichotoma seedling is up to 95% or above, and a transplantation survival rate is 92% or above. Compared with traditional seed propagation, cutting seedling or seedling culture, tissue culture is more favorable for realizing the engineering seedling of the cordia dichotoma, the defects of slow growth and long culture period of the cordia dichotoma are overcome, the method is favorable for promoting plantation of the cordia dichotoma, and a new thought is provided for thefurther expanded culture of the cordia dichotoma.

Description

Technical field [0001] The present invention relates to the technical field of plant cultivation, and in particular to a tissue cultivation method of ragwood. Background technique [0002] Cordia dichotoma Forst. f. is a tree of the genus Cordia dichotoma of the Boragaceae family. The tree can reach a height of 20 meters. It blooms from February to April and bears fruit from June to August. The color is dim when the old leaves wither. , named after the frequent holes. Also known as Qingtong tree, Qingtong green tree, paper kite tree, also known as Pobuzi, Shuzi, Shuzizi, Poguozi, Pozi, Broken axe, Xianzhizi and Kitezi; distributed in southeastern Tibet, Yunnan and Guizhou, China , Guangxi, Guangdong, Fujian and Taiwan. It is distributed in Vietnam, northern India, northeastern Australia and New Caledonia. It grows in sparse forests on hillside and beside streams in valleys at an altitude of 300-1900 meters. This fruit is rich in fat and can be squeezed for oil. According...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G17/00
CPCA01G17/005A01H4/001A01H4/008
Inventor 王远能
Owner 王远能
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