Biological activator for reinforcing nitrogen fixing capacity of nodule bacteria

An indigenous root nodule and biological source technology, applied to biocides, microorganisms, animal repellents, etc., can solve the problems of low nitrogen fixation capacity, rhizobia agent invasiveness, low nodulation rate, etc., to achieve increased iron content, The effect of increasing the fresh dry weight and nitrogen fixation activity of root nodules and improving product quality

Inactive Publication Date: 2008-09-03
HUNAN PLANT PROTECTION INST
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Problems solved by technology

[0004] In the cultivation of leguminous crops, the invention provides a method for enhancing the nitrogen-fixing ability of native rhizobia, aiming at the defects that artificially inoculated rhizobia agents have low infectivity and nodulation rate, while native rhizobia have strong nodulation rate but low nitrogen-fixing ability. biogenic activator

Method used

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  • Biological activator for reinforcing nitrogen fixing capacity of nodule bacteria
  • Biological activator for reinforcing nitrogen fixing capacity of nodule bacteria
  • Biological activator for reinforcing nitrogen fixing capacity of nodule bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Preparation Example

[0020] (1) Trichoderma harzianum strain (Trichoderma harzianum) T216 parent species slant culture: adopt test tube PDA slant medium culture;

[0021] (2) Trichoderma seed shake flask culture, medium formula: soluble starch 30g, glucose 20g, peptone 5g, KH2PO4 1g, MgSO4 7H2O 0.5g, CaCO3 0.25g, (NH4)2SO4 2g, water 1000mL, pH6.9, Divide into 500mL Erlenmeyer flasks with a liquid volume of 200mL, sterilize at 115°C for 20 minutes, transfer the parent species to shake flasks under aseptic conditions, and culture at 27±1°C at 150 rpm for 60 Hours, get the seeds, standby; fermenter fermentation, access fermenter by 10% (V / V) inoculum size, carry out controlled fermentation, fermentation control parameters are: temperature control, divided into two stages, fermentation first stage bacteria Body culture stage 0-72 hours, 26-28 °C, fermentation second stage metabolite production 72-100 hours, 23-25 ​​°C; ventilation (V / V): 0-20 hours: 1:0.6; 20-40...

Embodiment 4

[0045] The influence of embodiment 4 biological source T216 activators on the biological characteristics of indigenous rhizobia

[0046] (1) Acquisition of indigenous rhizobia

[0047] Collect cowpea plants at the early flowering stage grown under natural conditions in the field, pick off fresh, plump, and large root nodules on them, rinse them with tap water, soak them in 75% alcohol for 1 minute, rinse them with sterilized water for 3 times, and then put them Disinfect the surface in 0.1% mercuric chloride solution for 5 minutes, rinse with sterilized water for several times, put the nodules into a sterilized mortar filled with a small amount of sterilized normal saline (8.5 grams of sodium chloride in 1 liter of water) , use a glass rod to crush the root nodule; use an inoculation loop to dip the root nodule sap and draw a line on a plate containing Congo red Y.E.M. Rhizobia colonies, and the colonies were purified, cultivated and identified.

[0048] The composition of t...

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Abstract

A biological source activator for enhancing nitrogen fixation ability of native rhizobia is disclosed, which is prepared by method of: (1) slant culturing T216 mother fungi of trichoderma harziarum strain; (2) fermenting in fermentation jar; (3) adjusting pH to 7-8 with sodium hydroxide solution after fermentation, sealing the jar for one night, vacuum filtering with sterilized gauze, obtaining filtrate, sealed packaging. Before seeding of legume crops, the inventive biological source activator is added into seed soaking liquid in accordance of 20-30% of volume ratio and seed is soaked for 4-6 hours, or when seedlings of legume crops are cultured, the biological source activator is diluted 4-5 times and root is soaked for half a hour, thus when crops are growing, root nodulation of native rhizobia is induced, and nitrogen fixation ability is evidently increased.

Description

technical field [0001] The invention relates to a biological source activator of rhizobia nitrogen fixation ability, in particular to a biological source activator which uses Trichoderma and its metabolites to enhance the nitrogen fixation ability of indigenous rhizobia. Background technique [0002] In the symbiotic nitrogen fixation system of rhizobium-legume crops, symbiotic nitrogen fixation is a complex process that is jointly participated, synergistically and independently regulated by the relevant genes of both parties. Factors that affect and determine the symbiotic nitrogen fixation efficiency of rhizobia include soil ecological environment, Three aspects, such as the host plant and the genetic characteristics of the rhizobia themselves. As far as rhizobia are concerned, the number of indigenous rhizobia and the acid resistance of rhizobia (previous studies using fluorescent antibody method have found that as the degree of soil acidification increases, the degree of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/02C12R1/885
Inventor 梁志怀魏林罗赫荣魏宝阳陈玉荣申爱荣
Owner HUNAN PLANT PROTECTION INST
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