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Application of florigen gene GmFT2a

A florigen and gene technology, applied in application, genetic engineering, plant genetic improvement, etc., to achieve the effect of increasing biomass and enriching theoretical knowledge

Inactive Publication Date: 2018-11-20
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, for FT The functional studies of , mainly focused on the flowering process in the upper part, about FT The research on the regulatory relationship with the underground root system and the unique root nodules of legumes has not been reported yet

Method used

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  • Application of florigen gene GmFT2a
  • Application of florigen gene GmFT2a
  • Application of florigen gene GmFT2a

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Embodiment Construction

[0021] In order to make the content of the present invention easier to understand, the technical solutions of the present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited thereto.

[0022] 1. The florigen gene GmFT2a Expression pattern and tissue localization analysis

[0023] 1. The florigen gene GmFT2a Analysis of the expression pattern of

[0024] GmFT2a The length of the open reading frame of the gene is 531bp, encoding a protein of 176 amino acids, belonging to a subfamily of the PEBP family. To be clear GmFT2a Whether it is expressed in root nodules and in different tissues at different growth stages, its expression pattern was analyzed first.

[0025] Soybean seeds Willimas 82 with 3% (v / v) H 2 o 2 Disinfect the surface for one minute, germinate in quartz sand wetted with 1 / 2 full nutrient solution for 7 days, transfer to low-nitrogen nutrient solution [(LN: 530 μM NH 4 NO 3 +KNO 3 +Ca...

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Abstract

The invention discloses new application of a florigen gene GmFT2a to improvement of biological nitrogen fixation efficiency of leguminous plants. The florigen gene GmFT2a is mainly expressed in laterdevelopment period of root nodules and is positioned in cell nucleus and cytoplasm of neoplastic cortical cells, so that the number and weight of the soybean root nodule are significantly increased, the activity of dinitrogenase is significantly improved, the nitrogen content and the phosphorus content of plants are increased, and the biomass of transgenic plants is finally increased. Efficient utilization of nitrogen and phosphorus nutrients of leguminous crops can be realized, gene resources are provided for high-yield molecular breeding as well as genetic improvement of photoperiodicity, and important guidance and practical significance in developing fertilize-reducing and effect-improving sustainable ecological agriculture are achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to new applications of genes, in particular to florigen genes GmFT2a New applications in promoting biological nitrogen fixation in leguminous plants and improving nitrogen and phosphorus efficiency. Background technique [0002] Soybean is a typical nitrogen-fixing crop. However, the formation of nitrogen-fixing organs—root nodules is the basis for biological nitrogen fixation. Therefore, studying the physiological and molecular regulation mechanisms of soybean nodulation and nitrogen fixation, and exploring the potential of soybean biological nitrogen fixation are of great significance for the genetic improvement and molecular breeding of soybean nitrogen fixation efficiency. [0003] Flowering is a typical sign of plant transition from vegetative growth to reproductive growth, and it is also an essential element for the formation of soybean yield. Among them, Flowering Locus T ( FT ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/06A01H6/54
CPCC07K14/415C12N15/8243
Inventor 李欣欣廖红艾文琴
Owner FUJIAN AGRI & FORESTRY UNIV
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