Stress resistance and nitrogen fixation bradyrhizobium sp. suitable for northeast area and application thereof

A technology of bradyrhizobium and nitrogen fixation, applied in the field of microorganisms, to promote green, healthy and sustainable development, reduce pollution, and expand germplasm resources of growth-promoting bacteria for grain crops in China

Active Publication Date: 2019-11-19
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to ensure the nitrogen-fixing ability of Rhizobium phagoides with maximum efficiency, it is necessary to screen highly efficient nitrogen-fixing rhizobia strains that have the ability to adapt to different ecological environments, have strong str

Method used

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  • Stress resistance and nitrogen fixation bradyrhizobium sp. suitable for northeast area and application thereof
  • Stress resistance and nitrogen fixation bradyrhizobium sp. suitable for northeast area and application thereof
  • Stress resistance and nitrogen fixation bradyrhizobium sp. suitable for northeast area and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Example 1, Functional Analysis of Peanut Bradyrhizobium sp. DBPB1

[0096] 1. Isolation of strain DBPB1

[0097] The invention collects peanut plants from Northeast China, separates and purifies bacterial strains from root nodules of peanut plants, identifies and obtains bacterial strain DBPB1.

[0098] Bacterial strain DBPB1 was cultivated in YMA solid medium, colony morphology: colony white, round, smooth and opaque surface ( figure 1 ), Gram staining was negative, the bacteria were rod-shaped, without spores ( figure 2 ).

[0099] 2. Analysis of strain DBPB1

[0100] 1. Security testing

[0101] The strain DBPB1 was inoculated on blood agar plate medium, cultured at 37°C for 7 days, and observed for hemolysis circles. If there is a hemolytic circle, it means that the strain has hemolytic activity and is a potential threat to humans and animals, so the strain should be prohibited from being used in microbial fertilizers; if there is no hemolytic circle, it mean...

Embodiment 2

[0129] Example 2, field application research of Bradyrhizobium sp. DBPB1 in peanut

[0130] 1. Field application method of Bradyrhizobium sp. DBPB1

[0131] 1. Strain activation and expansion culture: Bradyrhizobium sp. DBPB1 strain stored in a -80°C refrigerator was activated on YMA solid medium by streaking, cultured in a 28°C constant temperature incubator, and a single colony was isolated . Use a sterile inoculation loop to pick a single colony into YMA liquid medium for expansion culture, place it at 28°C, and shake it at 180 rpm for about 7 days until the concentration of the bacterial solution is 1×10 10-11 About cfu / ml (OD 600 =1.2 or so), to obtain the bacterial agent mother liquor;

[0132] 2. Before use, dilute the bacterial agent mother solution with clean tap water to three times the volume to obtain the bacterial solution and mix it evenly, and then add the trace element solution (the amount of the trace element solution added to the diluted bacterial solution...

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Abstract

The invention discloses stress resistance and nitrogen fixation bradyrhizobium sp. suitable for the northeast area and an application thereof. The invention firstly discloses bradyrhizobium sp. DBPB1;the bradyrhizobium sp. DBPB1 has the preservation number of CGMCC No.17502 in China General Microbiological Culture Collection Center. The invention further provides a bacterial agent including the bradyrhizobium sp. and an application thereof. The bradyrhizobium sp. DBPB1 has the characteristics of promoting growth, has high adaptability, is a biosafe strain, can significantly increase the plantheight, the number of root nodules, the fresh weight on the ground and other traits of peanut in different development periods, greatly improves the yield of peanut, and provides the northeast area,one of main peanut planting areas in China, with microbial germplasm resources with high efficient nitrogen fixation and stable growth promotion, and is conducive to promoting green, healthy and sustainable development of agriculture in China.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of bradyrhizobium suitable for stress resistance and nitrogen fixation in Northeast China and its application. Background technique [0002] Peanut is one of the important economic crops and oil leguminous crops in China. Peanuts are planted all over China, mainly in the Huanghuai region, Northeast China, Guangdong and Guangxi, etc. Peanut production in China mainly relies on chemical fertilizers, and does not use the "natural nitrogen fertilizer factory" of peanut rhizobia agents and peanut symbiotic nitrogen fixation, which is very distressing. The main reasons for the excessive use of chemical fertilizers are as follows: 1. Substitute products for chemical fertilizers are outdated; 2. Fertilization equipment is poor and fertilizer losses are large; 3. For legume crops, they are completely dependent on chemical nitrogen fertilizers, resulting in great waste; 4. The rese...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01C1/00A01C1/06C05G3/00C12R1/01
CPCA01C1/00A01C1/06C05D9/02C05G3/00C12N1/20C12N1/205C12R2001/01
Inventor 隋新华霍斌米国华尚佼颖吴月陈腊李可可马钧
Owner CHINA AGRI UNIV
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