Gene GmSPX5 for regulating and controlling growth of leguminosae root nodules, and application of gene GmSPX5

Abstract

The invention discloses a gene GmSPX5 for regulating and controlling growth of leguminosae root nodules, and the application of gene GmSPX5. The nucleotide sequence of the gene is as shown in SEQ ID NO.1 and the coding amino acid sequence is as shown in SEQ ID NO.2. The research shows that excessive expression of the GmSPX5 can increase the number of the leguminosae root nodules and the density ofroot nodules infecting cells under high phosphorus treatment; furthermore, the nitrogen phosphorus content and the yield of soybeans can be remarkably increased. Therefore, the GmSPX5 can regulae andcontrol the growth and development of the root nodules and has important effects of improving the nitrogen phosphorus nitration of plants and increasing the yield; and the gene GmSPX5 can be used forregulating and controlling the adaptability on phosphorus deficiency and nitrogen deficiency in soil by the plants through a transgenic technology, also can be applied to leguminous crop nitrogen phosphorus synergistic and efficient genetic improvement and has a very important market prospect.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering. More specifically, a gene that regulates legume root nodule growth GmSPX5 and its application. Background technique [0002] In recent years, there have been more and more research reports on SPX proteins, and the progress is very fast. Many studies have proved that proteins containing SPX domains are involved in phosphorus signaling and phosphorus balance regulation (Duan et al., 2008; Wang et al., 2009; Gu et al., 2012). Arabidopsis SPX There are 4 genes, namely AtSPX1 ~ AtSPX4 (Duan et al., 2008), where the overexpressed AtSPX1 Increased phosphorus starvation-induced genes such as ACP5 , PAP2 as well as RNS1 The expression of , which increases the phosphorus concentration, indicates that AtSPX1 Involved in the regulation of phosphorus starvation signaling at the transcriptional level (Duan et al., 2008). Instead, the mutation Atspx3 able to increase AtP...

AI Technical Summary

Problems solved by technology

However, there are few studies on the molecular mechanism of nodule adaptation to phosphorus deficiency, especially the key regulators of nodule phosphorus signaling.

Previous studies have shown that soybean wxya In the gene family, there are 6 members whose expression is up-regulated by phosphorus deficiency in root nodules (Yao et al., 2014b), but there is no corresponding gene function report so far

Images