New application of cell wall expansion protein gene gmexpb6

A technology of expanding proteins and cell walls, which is applied in the fields of application, genetic engineering, and plant genetic improvement to achieve the effect of increasing the nitrogen content of plants

Inactive Publication Date: 2017-03-22
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there has been no report on the involvement of expansins in the regulation of soybean root nodule organ enlargement

Method used

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  • New application of cell wall expansion protein gene gmexpb6
  • New application of cell wall expansion protein gene gmexpb6
  • New application of cell wall expansion protein gene gmexpb6

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Embodiment Construction

[0014] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0015] 1. Analysis of GmEXPB6 gene expression pattern

[0016] In the published soybean genome database, through homologous comparison, it was predicted that there are 9 members in the soybean cell wall expansion protein family, and the GmEXPB6 gene was determined to be a strongly expressed gene in root nodules by quantitative PCR technology. The length of the open reading frame of the GmEXPB6 gene is 834bp, which encodes a protein of 278 amino acids, which belongs to the β class of cell wall expansion proteins. Existing studies have shown that this gene is closely related to nodule enlargement, and its expression pattern was first analyzed.

[0017] Soybean seed HN66 with 3% (v / v) H 2 o 2 The surface was disinfected for one minute, and germination was accelerated for 5 days in quartz sand wetted with 1 / 2 full nutrient solution, and th...

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Abstract

The invention discloses new application of a cell wall expansin gene GmEXPB6, and particularly discloses new application of the GmEXPB6 gene on the aspects of promoting the biological nitrogen fixation of a leguminous plant and improving the nitrogen fixation efficiency. The inventors clone the gene GmEXPB6 with low-phosphorous enhancement and root nodule high expression through a quantitative PCR result and prove that the protein coded by the GmEXPB6 gene has the functions of promoting root nodule expansion, enhancing the root nodule nitrogenase activity and the like, and the root nodule nitrogen fixation efficiency and biomass of a transgenic compound plant are finally improved through GmEXPB6 overexpression. The study of the inventors can provide the gene resource for the nitrogen high-efficiency and high-yield molecule breeding of crops which include soybeans, and has important theoretical and practical significance on developing environmental-friendly sustainable agriculture.

Description

technical field [0001] The invention relates to a new application of genes, in particular to a new application of cell wall expansion protein gene GmEXPB6 in promoting biological nitrogen fixation of leguminous plants and improving nitrogen efficiency, and belongs to the field of biotechnology. Background technique [0002] Nitrogen is an important component of amino acids, proteins, and nucleic acids in plants, and is one of the macronutrients necessary for plant growth and development. With the development of agricultural production, the amount of nitrogen fertilizer application has increased dramatically. According to research reports, the application of nitrogen fertilizer is the main source of nitrogen in farmland soil in my country, but the average utilization rate of nitrogen fertilizer by plants is only about 40%. (Qiu Jianjun et al., 2012). A large amount of nitrogen fertilizer not only increases agricultural costs and wastes resources, but also causes eutrophicati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 廖红李欣欣赵静
Owner SOUTH CHINA AGRI UNIV
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