19results about How to "Improve nitrogen fixation capacity" patented technology

The invention discloses a CAPE polypeptide released by a protein coded by a soybean disease course related gene GmPR1-6 and application of the CAPE polypeptide. The nucleotide sequence of the soybean disease course related gene is as shown in SEQ ID NO.1, the amino acid sequence of protein coded by the soybean disease course related gene is as shown in SEQ ID NO.2, and the sequence of CAPE polypeptide released at the C terminal of the protein is as shown in SEQ ID NO.9. The soybean disease course related gene GmPR1-6 provided by the invention is a gene coding a core protein participating in soybean immune defense reaction, the C tail end of the GmPR1-6 protein can be sheared to release CAPE polypeptide, the CAPE polypeptide is externally applied to weaken early immune defense reaction triggered by soybean during infection of rhizobium, the weight and size of a single root nodule are increased, and the disease resistance of the soybean is improved. The symbiotic nodulation and nitrogen fixation capabilities of the soybeans are improved, and the method has potential application value in planting of leguminous plants.

The invention provides an artificial algae crust-based desertification land restoration method, which comprises the following steps: screening algae species, carrying out artificial culture and resistance exercise on the screened species to obtain enough available biomass, treating the obtained biomass, adding auxiliary materials to prepare an artificial algae crust inoculum, and carrying out artificial algae crust-based desertification land restoration on the artificial algae crust inoculum. And inoculating the inoculum to a to-be-repaired area, and maintaining until algae crust is formed. According to the method, the artificial algae crust with similar species structure, function and physical characteristics as the algae crust naturally formed in the field is rapidly formed through human intervention, and can automatically grow and develop after colonization, so that the restoration treatment of the desertification land is realized; the method effectively solves the problems of microbial death of the inoculum caused by too strong stress generated by a field environment, low colonization success rate caused by instability of an inoculation area, and difficult growth and expansion of the inoculum caused by rapid dehydration of the inoculum due to poor water retention capacity of the desertification land, and is an environment-friendly, sustainable and efficient novel desertification organism treatment method.

The invention discloses a three-season cultivation method for intercropping fresh corn with green soy beans and crop rotation of vegetables, relates to the field of agricultural planting, and aims to solve the problems that an existing planting method cannot fully utilize the characteristics of the fresh corn to achieve the yield increasing target and the planting income of the fresh corn is limited. The method comprises the following steps: 1, land selection: selecting fertile, flat and well-drained soil for planting; 2, corn seed selection: selecting a suitable fresh corn variety according to climate conditions of a planting area; 3, planting two seasons of fresh corn in one year, and intercropping green soy beans while planting the fresh corn; fourthly, after the fresh corn is harvested, ploughing, soil preparation and ridging are conducted, and then winter vegetables are planted; 5, performing field management and pest control. According to the method, the growth characteristic that the fresh corn is short in growth cycle is fully utilized, two-season fresh corn can be planted in one year, the planting income of the fresh corn is increased, and the fresh corn is promoted to meet marketization requirements.

The invention relates to a saline-alkali soil modifier based on lucid ganoderma residue compost and a preparation method and application thereof, belongs to the technical field of soil improvement, and provides the saline-alkali soil modifier based on lucid ganoderma residue compost, which comprises the following components in parts by weight: 500-700 parts of lucid ganoderma residue compost and 25-50 parts of a saline-alkali tolerant nitrogen-fixing microbial agent. According to the saline-alkali soil improver prepared on the basis of the ganoderma lucidum residue aerobic compost and the saline-alkali-resistant nitrogen-fixing microbial inoculum, organic solid wastes such as traditional Chinese medicine residues are recycled; the improvement effect is good, the leaching efficiency of the saline-alkali soil is increased, the diversity of soil microbial communities is increased, and the enzyme activity is increased; the crop yield is greatly improved, and the crop nutritional quality is improved; compared with other bio-organic fertilizers, the bio-organic fertilizer has the advantage of reducing emission of greenhouse gas N2O.

The invention belongs to the technical field of biology, and relates to novel application of protein. The invention discloses an NPE1 protein and an application of a biological material for coding the NPE1 protein in regulating and controlling the catalytic efficiency of a metabolic enzyme. The overexpression of the new gene NPE1 of the soybean in the soybean can obviously enhance the catalytic efficiency of key metabolic enzymes GmPGK3a, GmPFK3a, GmGS1gamma1 and GmPGD2a in root nodules, so that the nitrogen fixation capacity of the soybean root nodules is enhanced, and the biomass and the final yield of overground parts of plants are further improved; the research of the inventor provides gene resources for nitrogen efficiency and molecular breeding of leguminous plants including soybeans, and provides a theoretical basis for molecular design of efficient nitrogen fixation soybean varieties, so that the development of environment-friendly green sustainable agriculture is promoted.

The invention discloses a preparation method for producing a biological bacterial fertilizer from lincomycin waste mushroom dregs, and relates to the technical field of fertilizer preparation, and the preparation method comprises the following steps: pretreatment of lincomycin waste mushroom dregs, microbial inoculation, strain fermentation, trace element addition and liquid fertilizer finished product detection. The method has the advantages that the waste lincomycin residues are alternately soaked with diluted hydrochloric acid and a sodium hydroxide solution, various impurities on the surfaces and in the waste lincomycin residues can be fully dissolved, alkaline harmful substances and pollutants combined with metal ions can be decomposed under the acidic condition, and the waste lincomycin residues can be recycled under the alkaline condition. The micro-bubbles can decompose acidic residual antibiotics and organic substances, in the continuous aeration process, on one hand, the micro-bubbles can scour the surfaces of mushroom dregs to enable impurities to fall off and be dissolved more easily, on the other hand, the content of dissolved oxygen in liquid is increased, favorable conditions are created for follow-up microorganism survival, and meanwhile oxidative decomposition of harmful substances is facilitated.

The invention relates to the technical field of biological cells, and discloses a biologically fermented soil conditioner and a preparation method thereof.The soil conditioner is prepared from, by weight, 3 + / -2 parts of water dispersible nano sulfur-containing colloidal suspension, 70 + / -10 parts of compound amino acid solution, 20 + / -5 parts of compound ion mineral substance and 7 + / -2 parts of organic acid; according to the method, the soil conditioner is added into the soil, ionic mineral substances in the conditioner can rapidly promote ATP generation of indigenous microorganisms to promote microbial breeding gene change and shorten the breeding cycle, indigenous flora proliferation in the soil can be activated, the indigenous microorganisms are greatly proliferated to enable a soil layer to generate a fermentation effect, and the soil layer is protected from being damaged. The generated aerobic bacteria can multiply to kill anaerobic bacteria, and the multiplication of the aerobic bacteria not only can improve the soil fertility, potassium dissolving capacity, phosphorus dissolving capacity, salt dissolving capacity and nitrogen fixing capacity, but also can degrade heavy metal pollutants, so that the occurrence of soil-borne diseases is prevented and controlled, and the purposes of integrally repairing and improving the soil and increasing the crop yield are achieved.

The application provides a cold-adapted straw degrading and nitrogen-fixing composite microbial agent, a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms.The cold-adapted straw degrading and nitrogen-fixing composite microbial agent comprises Acinetobacter guillouiae JIA101, Paenibacillus polymyxa JIA102, Raoultella sp.JIA201, Cellulosimicrobium cellulans JIA202 and Raoultella sp.JIA203.The composite microbial agent has high enzyme activity, straw degrading capacity and nitrogen-fixing capacity, and is suitable for a low-temperature environment.Pot experiment results show that under the condition of 10 DEG C, the microbial agent can significantly promote straw degradation, the straw degradation rate in soil reaches 44.99%, and is increased by 88.49% compared with a control group;and the treatment can effectively improve the soil nutrient and organic matter content.The application provides technical support for straw returning to field and soil fertilization under a low-temperature environment.

The invention provides application of hydroxypropyl chitosan oligosaccharide in preparation of a plant growth regulator, and belongs to the technical field of plant growth. The hydroxypropyl chitosan oligosaccharide is used as a raw material to prepare the plant growth regulator, new application of the hydroxypropyl chitosan oligosaccharide is provided, the seedling height of plants can be effectively increased, the root length can be effectively increased, the number and / or weight of root nodules can be effectively increased, the number of the root nodules of the plants and / or the activity of nitrogenase can be effectively increased, and the yield of the plants is increased. According to the present invention, the activity of glutathione reductase (GR) and / or glutathione transferase (GST) is improved so as to increase the reduced glutathione content, enhance the oxidation resistance of plants and / or enhance the salt resistance of crops, and after the compound fertilizer is compounded with the calcium-magnesium element fertilizer, the use amount of the compound fertilizer is reduced so as to provide effects of application reducing and effect increasing. The method for preparing the plant growth regulator is simple and easy to implement, environmentally friendly and convenient to popularize and apply.

In view of the problem of the prior art that with the increase of the fertilizer amount, the nitrogen content of the root system gradually increases, the peanut root system gradually reduces the nitrogen fixation capacity, and the fertilizer utilization rate is reduced and the environment is polluted, according to the biological characteristics that peanuts can produce root nodules to fix nitrogen, the application provides a kind of small molecule protein for improving peanut nitrogen fixation and yield and application thereof, the small molecule protein is any one or combination of two or more of 10 kinds of small molecule proteins (ENFP-1 to ENFP-10), and when used, an ENFP aqueous solution containing a Silwet series organosilicon surfactant is prepared. The experiment proves that: leaf spraying ENFP solution can significantly increase the number of peanut root nodules, improve the nitrogen fixation capacity of peanuts, increase the nitrogen content of peanut leaves, and can increase the yield of peanuts, and has a significant yield-increasing effect.

The invention relates to a method for promoting growth, nodulation and nitrogen fixation of indigofera pseudotinctoria by utilizing rhizobium D303 and a bacterial solution thereof. The biological preservation number of the rhizobium sp. D303 is CCTCC (China Center for Type Culture Collection) NO: M20252803. Pot culture tie-back tests prove that the indigofera pseudotinctoria seedlings inoculated with the rhizobium are obviously superior to a control group in the aspects of plant height, root length, overground dry weight, underground dry weight, root nodule number, root nodule weight, root nodule diameter, nitrogenase activity, antioxidant enzyme activity and the like. The rhizobium strain provided by the invention has efficient nodulation and nitrogen fixation capability and excellent growth promoting characteristic, obviously improves the growth speed, nodulation capability and nitrogen fixation efficiency of the indigofera pseudotinctoria, reduces the dependence on chemical nitrogen fertilizer, and has a broad application prospect in development of special biological bacterial fertilizer for indigofera pseudotinctoria. The method has important ecological and economic benefits for promoting the application of the indigofera pseudotinctoria in ecological restoration and feed production, and is suitable for slope greening, water and soil conservation and barren mountain ecological restoration projects.

The present invention discloses a method for repairing soil contaminated by electrolytic manganese slag by inoculating white rot fungi, comprising the following steps: mixing biomass and soil contaminated by electrolytic manganese slag to obtain compost raw materials; inoculating white rot fungi in the compost raw materials to obtain a pile; composting and fermenting the pile to obtain compost mature material, thereby completing the repair of soil contaminated by electrolytic manganese slag. The present invention effectively reduces the leaching amount of heavy metal manganese by inoculating white rot fungi, effectively reduces the mobility and bioavailability of heavy metal manganese, effectively changes the structure of microbial communities, increases the number and diversity of microorganisms, improves the seed germination index, and realizes the repair of soil in contaminated sites. It has the advantages of quickly starting composting repair, effectively reducing the leaching amount of heavy metal manganese, reducing the mobility and bioavailability of heavy metal manganese, increasing microbial diversity and community abundance, and improving the seed germination index.

The invention relates to a soil remediation and water retention microbial fertilizer, which is prepared by culturing a compound strain and optimizing nutrient substances in a culture dish, preparing the most suitable growth environment of the mixed strain, increasing the effective viable count of a microbial agent, and mixing the mixed agent with organic and inorganic fertilizers to form the compound microbial fertilizer with good water retention.

The invention discloses a soybean root nodule nitrogen fixation ventilation device and a using method thereof.The soybean root nodule nitrogen fixation ventilation device comprises a first nitrogen tank, a second nitrogen tank and a soybean planting box, the gas outlet end of the first nitrogen tank is provided with a first gas outlet branch pipe, the gas outlet end of the second nitrogen tank is provided with a second gas outlet branch pipe, and the first gas outlet branch pipe and the second gas outlet branch pipe communicate with a nitrogen main pipeline; the gas outlet end of the nitrogen main pipeline is connected with an L-shaped gas pipe, a plurality of nitrogen outlets are formed in the outer wall of the L-shaped gas pipe, nitrogen spraying assemblies are installed at the nitrogen outlets, the soybean planting box is arranged on one side of the second nitrogen tank, the L-shaped gas pipe is arranged at the bottom of the soybean planting box, planting soil is contained in the soybean planting box, and soybeans are planted in the planting soil. Nitrogen can be introduced into soybean planting soil, the nitrogen fixation efficiency of soybean root nodules can be improved, and then the purpose of efficient and high-quality cultivation of soybeans is achieved.

The invention discloses a soybean seed solid fungicide pre-inoculated coating and a preparation method thereof. The coating is prepared from an adhesive, a protective agent and compound microorganisms, the adhesive is vegetable oil; the protective agent is skim milk powder; the compound microorganism is any one or more than two of bacillus velezensis, soybean fast-growing type rhizobium and soybean slow-growing type rhizobium; the total bacterial count of the compound microorganisms is greater than or equal to 10 < 9 > cfu / mL. The invention solves the problem of poor application effect caused by low survival rate and short survival time of the existing microorganisms on the surface of the seed. According to the method, the rhizobium and various bacilli are compounded and pre-inoculated on the surfaces of the soybean seeds, so that the soybean sowing efficiency can be effectively improved, the nitrogen fixation capability of crops can be improved, and the stress resistance of the crops can also be improved. In addition, the compound microorganisms on the surfaces of the seeds can be stably stored for a long time, and the defects of low survival rate and short survival time of microorganisms on the surfaces of the seeds in the prior art are overcome.

The present application relates to the technical field of compound planting, in particular to a kind of southern winter idle field green manure optimization configuration efficient compound planting method, including step one, astragalus sinicus seed and rape seed banding sowing;Step two, astragalus sinicus seed and rape seed sowing promote growth treatment;Step three, astragalus sinicus and rape harvesting treatment;Step four, astragalus sinicus and rape crushing and turning back to field;Step five, strengthen and promote the management.Compared with prior art, a kind of southern winter idle field green manure optimization configuration efficient compound planting method is proposed in the present application, compared with traditional simple mixed sowing, in the yield of subsequent crop rice, rape straw decomposition rate, astragalus sinicus straw decomposition rate, >2mm soil aggregate formation, soil organic matter content and so on, there is greater improvement, it can be seen that the green manure optimization configuration efficient compound planting method is more conducive to soil fertilization and improve the yield of subsequent crop rice.

The present application relates to a kind of ecological soil capable of in-situ soil formation quickly and its preparation method and application.The ecological soil is composed of multi-source waste base and microbial inoculum;The mass ratio of multi-source waste and microbial inoculum is (18-20):1;Multi-source waste base is selected from one or more of coal gangue, fly ash, corn straw, cow dung and sugarcane residue;Microbial inoculum is composed of simple wheel branch bacteria fermentation liquor, fusarium fermentation liquor, spiral branch mould fermentation liquor, red ball bacteria fermentation liquor and green needle pseudeomonas fermentation liquor.The present application efficiently utilizes the waste generated by industrialization and urbanization, and on the basis of waste and microbial inoculum, realizes the synergistic efficient resource of multi-source waste, and the soil matrix prepared by composting is loose, rich in plant essential nutrients, and has strong water retention, has the advantages of promoting plant growth, strengthening ecological restoration, etc., and is suitable for mining subsidence area, reclamation site, sandy or saline-alkali farmland, facility agriculture and ecological greening fields.

The invention discloses a light carbon silicon humic acid compound fertilizer and a preparation method thereof, and belongs to the technical field of biological fertilizers. The light carbon silicon humic acid compound fertilizer is prepared from the following components in parts by weight: 15-20 parts of a light carbon silicon material, 10-20 parts of modified humic acid, 18-20 parts of urea, 12-15 parts of potassium chloride, 8-12 parts of monoammonium phosphate, 2-3 parts of a photosynthesis enhancer and 1-2 parts of a nanocrystallization aid, the optical carbon-silicon material is prepared by preparing a carbon carrier from a carbon raw material through a hydrothermal reaction and then loading a silicon element and photosynthesis promoting bacteria, the modified humic acid is prepared from a humic acid raw material through three-step treatment, fermentation, acid precipitation and hydrogen peroxide modification. The nanocrystallization auxiliary agent is an equivalent mixture of lauryl sodium sulfate and nano silicon dioxide. The light carbon silicon humic acid compound fertilizer provided by the invention promotes the growth and development of crops and improves the soil structure.

This invention relates to the field of agricultural fertilizer technology, and discloses a functional compound fertilizer containing a soil conditioner and its preparation method. The fertilizer provided by this invention is composed of nitrogen, phosphorus, and potassium sources, a compound soil conditioner, microencapsulated functional microbial agents, chelated trace elements, and a slow-release coating agent. The preparation method includes raw material pretreatment, step-by-step mixing, granulation, and coating processes. The fertilizer provided by this invention addresses the shortcomings of existing banana-specific fertilizers, such as poor synergy between soil conditioning and nutrient distribution, mismatch between fertilizer release and growth cycle, and limited stress resistance. It can precisely improve acidic banana plantation soil, achieve dynamic nutrient release, improve fertilizer utilization, reduce the incidence of banana wilt disease, significantly increase yield and fruit quality, is suitable for various cultivation models, and possesses both practical and environmental value.