Cotton drought resistant gene GhEXP1 and applications thereof

A gene and cotton technology, applied in the field of plant genetic engineering, can solve the problem that drought tolerance genes have not yet been reported, and achieve the effect of remarkable drought tolerance, great application value and improving drought tolerance.

Inactive Publication Date: 2015-10-21
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

However, these genes are all Arabidopsis genes, and the endogenous drought tolerance genes of cotton have not been reported yet.

Method used

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  • Cotton drought resistant gene GhEXP1 and applications thereof
  • Cotton drought resistant gene GhEXP1 and applications thereof
  • Cotton drought resistant gene GhEXP1 and applications thereof

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Embodiment Construction

[0023] The methods used in the following embodiments are conventional methods unless otherwise specified, and the primer sequences used are all synthesized by Shanghai Handsome Biotechnology Co., Ltd. The gene in this experiment was derived from the upland cotton variety Ao 3503 (Gossypium hirsutum L.) , this variety has strong drought tolerance.

[0024] (1) Cotton GhEXP1 gene cloning and sequence analysis

[0025] Cloning primers EXP1F: 5'-ACCAACCTTTATATTTGC-3', EXP1R: 5'-TTCCTTGTATCGGTGAAAG-3' were designed according to a drought-induced up-regulated gene sequence in upland cotton transcriptome sequencing, and amplified in the cDNA of upland cotton variety Ao 3503. The obtained fragment was constructed into the pMD19-T vector (Takara) for sequencing, and it was found that the inserted fragment was 599bp in length and contained an open reading frame of 396 nucleotides. The gene was named GhEXP1, and the T vector containing the gene Named T-EXP1.

[0026] (2) Induced expre...

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Abstract

The invention relates to a cotton drought resistant gene GhEXP1 and applications thereof, and belongs to the biology technical field. The related gene GhEXP1 is a cotton expansin gene and is reported for the first time. The gene is cloned from an upland cotton draught resistant species (Ao 3503), and through RT-PCR analysis, people find that the expression of GhEXP1 is up-regulated after a drought treatment. The drought resistant performance of strains containing silenced GhEXP1 gene is obviously reduced. The living rate of GhEXP1 transgene arabidopsis plants is 80% after a 30-day drought treatment; while the living rate of referential wild arabidopsis is 10%; and the result shows that the GhEXP1 gene can prominently improve the drought resistant performance of plants. The GhEXP1 gene is advantageously used for improving the plant drought resistance and has a wide application prospect.

Description

1. Technical field [0001] The invention provides a cotton drought-tolerant gene GhEXP1 and its application, belonging to the field of plant genetic engineering. It involves plant gene cloning and functional analysis, which is used to improve plant drought tolerance and other beneficial production traits through plant genetic engineering technology. 2. Background technology [0002] The loss caused by drought to plants is the first among all abiotic stresses, second only to the losses caused by diseases and insect pests in biotic stress. Drought can cause serious dehydration of plant cells, damage the normal cell membrane structure, lead to excessive closure of stomata, and then affect the CO 2 absorption, photosynthesis is reduced. Cotton is the most important economic crop in China and even in the world. Most of its production areas are arid and semi-arid areas. Insufficient water supply has become one of the main obstacles to cotton production. Wang Yanqin et al. (Wang ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
Inventor 刘廷利张保龙刘小双阚家亮杨郁文陈天子袁洪波凌溪铁
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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