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Application of protein and related biological material thereof in increasing plant yield

A protein and plant technology, applied in plant products, applications, plant peptides, etc., can solve the problems of limited production potential of bred varieties and reduction of arable land area

Inactive Publication Date: 2016-06-29
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the area of ​​arable land is gradually decreasing, and the potential for increasing production of the current bred varieties is limited. The world is facing a new round of food security challenges. Therefore, it is necessary to develop new varieties with greater potential for increasing production.

Method used

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  • Application of protein and related biological material thereof in increasing plant yield
  • Application of protein and related biological material thereof in increasing plant yield
  • Application of protein and related biological material thereof in increasing plant yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1, Functional Verification of TaSPL20-7D Gene

[0077] The total RNA of wheat (Triticum aestivum L.) variety Yanzhan 4110 seedlings was extracted with TRIZOL, and the first-strand cDNA (Invitrogen) was synthesized with the M-MLV reverse transcription kit, and the cDNA was used as a template for PCR amplification with primers F1 and R1 to obtain 1445bp PCR amplification product.

[0078] F1: 5'-AAGAGTTATTGCCAACGGAC-3' (positions 1-20 of Sequence 1);

[0079] R1: 5'-CTAATGTGGTGTTCTAACTGTGG-3' (reverse complement of positions 1423-1445 of Sequence 1).

[0080] After sequencing, the sequence of the PCR product is shown in sequence 1 in the sequence table, the 213-1379th nucleotide of sequence 1 is the coding region, and the gene shown by the 213-1379th nucleotide of sequence 1 is named as TaSPL20-7D gene, the protein encoded by the gene is named TaSPL20-7D, and its amino acid sequence is sequence 2 in the sequence list.

[0081] 1. Obtaining genetically modified ...

Embodiment 2

[0116] Example 2, the expression of TaSPL20-7D gene in different tissues

[0117] 1. Expression of TaSPL20-7D gene in different tissues

[0118] Different tissue parts were taken at different growth stages of wheat (Triticum maestivum L.) variety Yanzhan 4110 as samples, frozen in liquid nitrogen, and stored at -80°C for later use. The sample materials obtained include: root at seedling stage (SR), top meristem at seedling stage (SC), top meristem at 4-leaf stage (4C), top meristem at 6-leaf stage (6C), top meristem at greening stage (GC)), leaf at seedling stage (SL), root after booting (BR), node (BN), internode (BI), leaf sheath (BS), leaf (BL), ear (BE) (0.5 cm ear ( 0.5E), 2cm ear (2E), 3cm ear (3E), 6cm ear (6E), 8cm ear (8E), 10cm ear (10E)), ear at late booting stage (BE), ear at flowering stage (FE) , 4 days after anthesis (4G), 7 days after anthesis (7G), 14 days after anthesis (14G) and 21 days after anthesis (21G).

[0119] TRIZOL was used to extract the total R...

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Abstract

The invention discloses application of a protein and a related biological material thereof in increasing plant yield. The protein is the following A1) or A2) or A3): A1) protein of which the amino acid sequence is Sequence 2; A2) plant-yield-related protein obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence disclosed as Sequence 2 in the sequence table; or A3) fusion protein obtained by connecting a tag to the N terminal or / and C terminal of A1) or A2). The increase of the plant yield comprises improvement of plant yield related traits, including at least one of spike length, number of spike grains, weight of thousand-grain, grain length, grain width, primary rachis branch number and secondary rachis branch number. The experiment proves that the protein and coding gene thereof can be utilized to enhance the spike length, number of spike grains, weight of thousand-grain, grain length, grain width, primary rachis branch number and secondary rachis branch number of the plants, thereby increasing the plant yield.

Description

technical field [0001] The invention relates to the application of protein and related biological materials in improving plant yield in the field of biotechnology. Background technique [0002] The production of food is not only related to people's daily life, but also restricts the economic situation of the country to a certain extent. As my country is the most populous country in the world, it is particularly important to increase food production. In the past few decades, grain production has increased significantly. In addition to the improvement of cultivation and management conditions, the genetic improvement of crop varieties has played an important role in increasing grain production. However, in recent years, the increase in grain production has reached a bottleneck period. At present, the area of ​​arable land is gradually decreasing, and the production potential of the current bred varieties is limited. The world is facing a new round of food security challenges....

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8261
Inventor 景蕊莲徐伟娜张斌刘霞毛新国
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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