Method for identification or auxiliary identification of day age of pig at 100kg weight and special kit of method

A technology for identifying pigs and kits, applied in the field of genetic engineering

Active Publication Date: 2016-02-10
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

People's lives are constantly improving, and traditional farming techniques can no longer meet people's demand for pork

Method used

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  • Method for identification or auxiliary identification of day age of pig at 100kg weight and special kit of method
  • Method for identification or auxiliary identification of day age of pig at 100kg weight and special kit of method
  • Method for identification or auxiliary identification of day age of pig at 100kg weight and special kit of method

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Experimental program
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Embodiment 1

[0033] Embodiment 1, a kind of auxiliary identification pig reaches 100kg body weight day-old method

[0034] 1. Screening of SNP sites of porcine H19 gene

[0035] 1. Extraction of DNA from pig ear tissue samples

[0036] The blood samples of 32 Landrace pigs from Hebei pig farms were collected respectively. After the samples were collected, they were stored in 75% alcohol and stored at -20°C. The genomic DNA of blood samples was extracted by phenol-chloroform extraction, and the specific steps were as follows:

[0037] (1) Preparation of ear tissue samples: Cut ear tissue samples of moderate size, wash away impurities and blood stains on the surface with normal saline, put them in a 1.5ml centrifuge tube, cut them into pieces with ophthalmic scissors, and add 500uL of tissue to it Lysis solution (Beijing Baitaike Biotechnology Co., Ltd.; product number: AU19011);

[0038] (2) According to the size of the cut ear tissue sample, add 15-25uL proteinase K (SIGMA-ALDRICH; prod...

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Abstract

The invention discloses a method for identification or auxiliary identification of day age of a pig at 100kg weight and a special kit of the method. The method comprises the following steps: detecting whether deoxyribonucleotide on the 402nd site of H19 gene of a pig individual is either C or T or both of them, so as to determine whether the genotype of the pig individual is TT or CC or TC; and determining the day age of the pig at 100kg weight according to the genotype of the pig individual: the day age of the pig individual of the TT genotype at 100kg weight is more than that of the pig individual of the CC genotype, and the day age of the pig individual of the CC genotype at 100kg weight is more than that of the pig individual of the TC genotype. Tests prove that the method disclosed by the invention, which is used for identifying the day age of the pig at 100kg weight through single nucleotide polymorphism on the 402nd site of the H19 gene, is consistent with an actual determination result of the day age of the pig at 100kg weight; therefore, the method is of great significance to the selection of excellent pigs.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and specifically relates to a method for identifying or assisting in identifying pigs with a body weight of 100 kg and a special kit thereof. Background technique [0002] Pig is the first of the six livestock, and the pig industry occupies an important position in the world, especially in China's national economy. The proportion of pork in many meat products has been maintained at about one-third, and it is one of people's favorite non-staple foods. People's lives are constantly improving, and traditional breeding techniques can no longer meet people's demand for pork. With the development of modern breeding technology, the production performance of pigs has been significantly improved, and the healthy breeding and fattening of pigs is particularly important. [0003] The age of pigs reaching 100kg body weight is an important indicator for evaluating the growth rate of pigs, which can indire...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 唐中林孙浩李奎梁如意
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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