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Molecular marker for detecting day-age for achieving 100 kg pig, and applications thereof

A technology of body weight, DNA molecules, applied in the field of genetic engineering

Active Publication Date: 2017-09-12
INST OF ANIMAL SCI CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

People's lives are constantly improving, and traditional farming techniques can no longer meet people's demand for pork

Method used

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  • Molecular marker for detecting day-age for achieving 100 kg pig, and applications thereof
  • Molecular marker for detecting day-age for achieving 100 kg pig, and applications thereof
  • Molecular marker for detecting day-age for achieving 100 kg pig, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, a kind of auxiliary identification pig reaches 100kg body weight day-old method

[0045] One, the screening of the SNP site of porcine BMP4 gene

[0046] 1. Extraction of DNA from pig ear tissue samples

[0047] The blood samples of 32 Landrace pigs from Hebei pig farms were collected respectively. After the samples were collected, they were stored in 75% alcohol and stored at -20°C. The genomic DNA of blood samples was extracted by phenol-chloroform extraction, and the specific steps were as follows:

[0048] (1) Preparation of ear tissue samples: Cut ear tissue samples of moderate size, wash away impurities and blood stains on the surface with normal saline, put them in a 1.5ml centrifuge tube, cut them into pieces with ophthalmic scissors, and add 500uL of tissue to it Lysis solution (Beijing Baitaike Biotechnology Co., Ltd.; product number: AU19011);

[0049] (2) According to the size of the cut ear tissue sample, add 15-25uL proteinase K (SIGMA-ALD...

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Abstract

The present invention discloses a molecular marker for detecting the day-age for achieving a 100 kg pig, and applications thereof. The method for detecting the day-age for achieving a 100 kg pig by using the molecular marker comprises: detecting whether the genotype of an individual pig is TT or CC or TC, and determining the day-age for achieving the 100 kg pig according to the genotype of the individual pig, wherein the day-age of the TT genotype individual pig is more than the day-age of the CC genotype individual pig, and the day-age of the CC genotype individual pig is more than the day-age of the TC genotype individual pig. According to the present invention, the experimental results prove that the determination result of the method for identifying the day-age for achieving the 100 kg pig by using the 267 site single nucleotide polymorphism of the fourth intron of the BMP4 gene is consistent with the actual determination result so as to provide important significance for the selection of excellent pig breeders.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a molecular marker for detecting the age of a pig with a body weight of 100 kg and an application thereof. Background technique [0002] Pig is the first of the six livestock, and the pig industry occupies an important position in the world, especially in China's national economy. The proportion of pork in many meat products has been maintained at about one-third, and it is one of people's favorite non-staple foods. People's lives are constantly improving, and traditional breeding techniques can no longer meet people's demand for pork. With the development of modern breeding technology, the production performance of pigs has been significantly improved, and the healthy breeding and fattening of pigs is particularly important. [0003] The age of pigs reaching 100kg body weight is an important indicator for evaluating the growth rate of pigs, which can indirectly r...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 唐中林李奎梁如意杨亚岚张建斌
Owner INST OF ANIMAL SCI CAAS
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