Molecular marker for improving fecundity of Sunit sheep and application of molecular marker

A fecundity and sunite technology, applied in molecular markers for improving sunite sheep fertility and its application fields, can solve the problems of low incidence and the like

Active Publication Date: 2019-03-01
INNER MONGOLIA UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Guan Feng et al. used PCR-RFLP method to study GDF9 gene G8 mutation in Hu sheep, Charolais, Dorset, Suffolk, Chinese Merino meat multi-fetal lines, Chinese Merino sheep and Romney 7

Method used

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  • Molecular marker for improving fecundity of Sunit sheep and application of molecular marker
  • Molecular marker for improving fecundity of Sunit sheep and application of molecular marker
  • Molecular marker for improving fecundity of Sunit sheep and application of molecular marker

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Experimental program
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Effect test

Embodiment 1

[0035] Example 1. Establishment of a method for detecting molecular markers for improving the double lamb rate of Sunit sheep and its effect verification

[0036] 1. Establishment of a method for detecting molecular markers that improve the fertility of Sunit sheep

[0037] 1) template material preparation

[0038] The blood samples of Sunite sheep were collected and the fecundity and parity were recorded. The collected Sunite sheep came from the Sunite Left Banner of Xilingol League, Inner Mongolia. The blood samples were stored in anticoagulant tubes for later use.

[0039] 2) PCR product sequencing.

[0040] 20 Sunit sheep were randomly selected (10 consecutive double-lamb ewes + 10 consecutive single-lamb ewes) for PCR product sequencing, and the SNP site located in the GDF9 promoter was found, that is, -118 site.

[0041] 3) Primer design

[0042] According to the sheep gene sequence reported by GeneBank (GeneBank accession number: NC_019462.2), the upstream and downst...

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Abstract

The invention discloses a method for improving fecundity of Sunit sheep by gene GDF9 (growth differentiation factor 9). The genotype of Sunit sheep is determined by detecting nucleotide at the site 118 upstream of a promoter of the gene GDF9 in a genome of the to-be-tested Sunit sheep, and then fecundity of the Sunit sheep is determined according to the genotype; if the nucleotide at the site 118upstream of the promoter of a coding region of the gene GDF9 in the genome of the Sunit sheep is T, the genotype of homozygote is TT; when the 118th nucleotide upstream of the promoter of the gene coding region in the genome of the Sunit sheep is G, the genotype of homozygote is GG and the genotype of heterozygote is TG. Experiments prove that the average lambing number of the GG genotype Sunit sheep is higher than that of the GT genotype, and the average lambing number of the genotype GT Sunit sheep is higher than that of the TT genotype. Molecular marker polymorphism is detected by PCR-RFLP(restriction fragment length polymorphism) rapidly and simply, and an accurate and simple method is provided for improving fecundity of the Sunit sheep with the molecular marker assisted breeding technology.

Description

technical field [0001] A molecular marker for improving the fertility of Sunit sheep and its application. Background technique [0002] GDF9 (growth differentiation factor 9, GDF9) is growth differentiation factor 9, and BMP15 gene belongs to transforming growth factor β (TGFβ) superfamily members. The GDF9 gene of sheep is located on chromosome 5, with a total length of about 2.5kb, which contains 2 exons and 1 intron. Exon 1 is 397bp long, encoding 134 amino acids, and exon 2 is 968bp long. It encodes 456 amino acids, and the intron is 1126bp long. (Sadighi M, Bodensteiner K J, Beattie A E et al. Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheepchromosome 5. [J]. Animal Genetics, 2002,33(3):244-245.). [0003] Sonid sheep are produced in Sonid Left Banner and Sunid Right Banner of Xilin Gol League, Inner Mongolia Autonomous Region, known as Gobi sheep. Its main breeding method is natural grazing on pure natural pastures. The climate is characteriz...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 李光鹏佟彬杨燕影白春玲刘志刚吕菲菲
Owner INNER MONGOLIA UNIVERSITY
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