Molecular marker for improving fecundity of Sunit sheep and application of molecular marker

Abstract

The invention discloses a method for improving fecundity of Sunit sheep by gene GDF9 (growth differentiation factor 9). The genotype of Sunit sheep is determined by detecting nucleotide at the site 118 upstream of a promoter of the gene GDF9 in a genome of the to-be-tested Sunit sheep, and then fecundity of the Sunit sheep is determined according to the genotype; if the nucleotide at the site 118upstream of the promoter of a coding region of the gene GDF9 in the genome of the Sunit sheep is T, the genotype of homozygote is TT; when the 118th nucleotide upstream of the promoter of the gene coding region in the genome of the Sunit sheep is G, the genotype of homozygote is GG and the genotype of heterozygote is TG. Experiments prove that the average lambing number of the GG genotype Sunit sheep is higher than that of the GT genotype, and the average lambing number of the genotype GT Sunit sheep is higher than that of the TT genotype. Molecular marker polymorphism is detected by PCR-RFLP(restriction fragment length polymorphism) rapidly and simply, and an accurate and simple method is provided for improving fecundity of the Sunit sheep with the molecular marker assisted breeding technology.

Description

technical field [0001] A molecular marker for improving the fertility of Sunit sheep and its application. Background technique [0002] GDF9 (growth differentiation factor 9, GDF9) is growth differentiation factor 9, and BMP15 gene belongs to transforming growth factor β (TGFβ) superfamily members. The GDF9 gene of sheep is located on chromosome 5, with a total length of about 2.5kb, which contains 2 exons and 1 intron. Exon 1 is 397bp long, encoding 134 amino acids, and exon 2 is 968bp long. It encodes 456 amino acids, and the intron is 1126bp long. (Sadighi M, Bodensteiner K J, Beattie A E et al. Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheepchromosome 5. [J]. Animal Genetics, 2002,33(3):244-245.). [0003] Sonid sheep are produced in Sonid Left Banner and Sunid Right Banner of Xilin Gol League, Inner Mongolia Autonomous Region, known as Gobi sheep. Its main breeding method is natural grazing on pure natural pastures. The climate is characteriz...

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Problems solved by technology

[0006] Guan Feng et al. used PCR-RFLP method to study GDF9 gene G8 mutation in Hu sheep, Charolais, Dorset, Suffolk, Chinese Merino meat multi-fetal lines, Chinese Merino sheep and Romney 7 sheep The distribution in the population, it was found that the G8 homozygous mutation was only detected in the Hu sheep population, but the incidence rate was extremely low (0.645%, 2 / 310)

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