Method and special product for assisted identification of swine backfat thickness character

An auxiliary identification and backfat thickness technology, which is applied in biochemical equipment and methods, microbiological measurement/inspection, animal husbandry, etc., can solve the problems of slow breeding speed and low accuracy, achieve high accuracy and reduce breeding costs , the effect of cost is not

Inactive Publication Date: 2013-09-25
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Due to the slow speed and low accuracy of traditional breeding, molecular breeding has b

Method used

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  • Method and special product for assisted identification of swine backfat thickness character
  • Method and special product for assisted identification of swine backfat thickness character
  • Method and special product for assisted identification of swine backfat thickness character

Examples

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Embodiment 1

[0027] Embodiment 1, the backfat thickness trait of auxiliary identification pig

[0028] 1. Determination of g.47033C>T polymorphism site of porcine PPARD gene

[0029] 1. PCR amplification

[0030] According to the genome DNA sequence (GenBank Accession Number GU565976.1) information of pig PPARD gene, a pair of primers were designed as follows:

[0031] U (upstream primer): 5'-caaaacgcagtagcaggaaa-3' (sequence 1 of the sequence listing);

[0032] D (downstream primer): 5'-cccaacctctctagcacacc-3' (SEQ ID NO: 2 of the Sequence Listing).

[0033] Three Damin hybrid pigs were selected as experimental materials. Using the genomic DNA of each Damin hybrid pig as a template, PCR amplification was performed using primers U and D.

[0034] The amplification system is: 200ng of genomic DNA, 5μl of 10×PCR amplification buffer, 10mM of dNTPs, 50ng of upstream and downstream primers, 0.75U of Taq DNA polymerase, Mg 2＋ 2.5mmol / L, with ddH 2 O Supplement the reaction system to 50 μl...

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Abstract

The invention discloses a method and a special product for the assisted identification of swine backfat thickness character. The method for the assisted identification of swine backfat thickness character comprises the following steps of: determining whether the deoxyribonucleotide 47033 of the GenBank Accession Number GU565976.1 of a peroxisome proliferator-activated receptor delta gene of a to be detected swine is T, or C, or T and C, so as to determine whether the genotype of the to be detected swine is TT or TC or CC, and determining the backfat thickness character according to the genotype of the to be detected swine, wherein the backfat thickness of the to-be-detected swine with the TT genotype is greater than that of the to be detected swine with the TC genotype, and the backfat thickness of the to be detected swine with the TC genotype is greater than that of the to be detected swine with the CC genotype. The method and the special product disclosed by the invention are used for breeding swine, so that the to be detected swine can be screened in the early stage, the problem of long time needed for selecting good breeding swine in the practical production can be effectively solved, the breeding cost is reduced, and the swine backfat thickness in the practical production is effectively reduced or increased.

Description

technical field [0001] The invention relates to a method for assisting identification of pig backfat thickness traits and a special product thereof. Background technique [0002] One of the most famous characteristics of local pig breeds in my country is their excellent meat quality. These local breeds, breeds containing the blood of local breeds, and even Chinese and foreign hybrid commercial pork are widely welcomed by consumers because of their excellent meat quality, such as Beijing Black pork, Laiwu black pork, Heishan pork, Sutai pork, etc. are well received by consumers. Moreover, the price of these high-quality pork is 1.5-3 times that of ordinary pork, which fully reflects the commercial value of local breeds of pigs in my country at the current stage. However, most of these pig breeds are fat pigs, and their notable feature is the thick backfat. Excessive backfat deposition not only leads to slow growth, but also does not meet the requirements of the current situa...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01K67/027
Inventor 王立贤张龙超王立刚赵克斌颜华
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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