A method for auxiliary detection of pig intramuscular fat content traits and its special kit

A technology for auxiliary detection of intramuscular fat, applied in biochemical equipment and methods, microbiological measurement/inspection, animal husbandry, etc., can solve the problems of reduced accuracy of breeding, increased breeding costs, slow genetic progress, etc., to achieve High accuracy, reduced breeding costs, and high application value

Inactive Publication Date: 2016-06-22
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, this meat quality trait can only be measured after the animal is slaughtered. Conventional breeding can only be measured by siblings or half-sibs. On the one hand, the cost of breeding is increased, and on the other hand, the accuracy of selection is reduced, and the genetic progress is slow.

Method used

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  • A method for auxiliary detection of pig intramuscular fat content traits and its special kit
  • A method for auxiliary detection of pig intramuscular fat content traits and its special kit
  • A method for auxiliary detection of pig intramuscular fat content traits and its special kit

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Embodiment 1

[0029] Example 1, Auxiliary identification of pig intramuscular fat content traits

[0030] 1. Determination of g.97782A>G polymorphism site of pig MYHC gene

[0031] 1. PCR amplification

[0032] According to the genome DNA sequence of pig MYHC gene (GenBankAGGessionNumberCU856557.2) information, a pair of primers were designed as follows:

[0033] U (upstream primer): 5'-TTCCTTTCCTGAGCCTGAGA-3' (sequence 1 of the sequence listing);

[0034] D (downstream primer): 5'-AAGTTCCGAAAAGCTCAGCA-3' (SEQ ID NO: 2 of the Sequence Listing).

[0035] Damin hybrid pigs (2 heads) were selected as experimental materials. Using the genomic DNA of Damin hybrid pig as a template, primers U and D were used for PCR amplification.

[0036] The amplification system is: Genomic DNA 200ng, 10×PCR amplification buffer 5μl, dNTPs 10mM, upstream and downstream primers 50ng each, TaqDNA polymerase 0.75U, Mg 2+ 2.5mmol / L, with ddH 2 O Supplement the reaction system to 50 μl.

[0037] The PCR reactio...

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Abstract

The invention discloses a method and special kit for auxiliary detection of pig intramscular fat content. The method disclosed by the invention aims at detecting whether the deoxyribonucleotide at the 97782th locus of GenBank AGGession of a pig in Number CU856557.2 is A or G and confirming whether the genotype of a pig to be detected is AA or GG; the intramscular fat content of a pig with the GG genotype is higher than a pig with the AA genotype; the AA genotype is a homozygote that the deoxyribonucleotide at the 97782th locus from a 5'-end of GenBank AGGession is A in Number CU856557.2; the GG genotype is a homozygote that the deoxyribonucleotide at the 97782th locus from a 5'-end of GenBank AGGession is G in Number CU856557.2. By adopting the method, the breeding expense is reduced, the pig intramscular fat content in practical content is effectively improved, and the economic benefits are increased. The method disclosed by the invention is simple to operate, high in accuracy and capable of achieving automatic detection and can play a great role in pig breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for assisting in the detection of pig intramuscular fat content traits and a special kit thereof. Background technique [0002] One of the most famous characteristics of local pig breeds in my country is their excellent meat quality. These local breeds, breeds containing the blood of local breeds, and even Chinese and foreign hybrid commercial pork are widely welcomed by consumers because of their excellent meat quality, such as Beijing Black pork, Laiwu black pork, Heishan pork, Sutai pork, etc. are well received by consumers. Moreover, the price of these high-quality pork is 1.5-3 times that of ordinary pork, which fully reflects the commercial value of local breeds of pigs in my country at the current stage. As we all know, an important factor for the popularity of local pig breed pork in my country is the high intramuscular fat content (IMF). The fat content in pork, es...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01K67/027
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王立贤张龙超王立刚赵克斌颜华
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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