129results about How to "Enables direct detection" patented technology

The invention relates to a method for detecting a nonzero digit compensation light-degree optical non-spherical profile. The method comprises the following steps of: calculating the value of z(x, y)-s(x, y, r), wherein z(x, y) is the rise distribution of an aspheric surface along the direction of an optical axis, and s(x, y, r) is the rise distribution of a spherical surface closest to the aspheric surface to be detected along the direction of the optical axis; converting emergent parallel light of an interferometer into standard spherical waves by utilizing a transmission ball and using the standard spherical waves as reference spherical waves; adjusting the position of the aspheric surface to be detected to ensure that the circle center of the spherical surface closest to the aspheric surface to be detected coincides with a focus point of converging the reference spherical waves; detecting phase distribution data of interference fringes formed by the optical waves reflected by the aspheric surface to be detected and the reference spherical waves by utilizing the interferometer; and rejecting the value of the z(x, y)-s(x, y, r) and an adjusting and positioning error epsilon (x, y) from the phase distribution data of the interference fringes to obtain the profile error distribution e(x, y) of the aspheric surface. The invention can realize the detection of large-caliber concave and convex light-degree aspheric surface profiles with high resolution and high precision and has the advantages of low detection cost and short detection cost.

The invention belongs to the technical field of human nucleic acid in vitro detection, and specifically relates to a PCR (polymerase chain reaction) amplification system for genotyping of three SNP (single-nucleotide polymorphism) loci related to human folic acid metabolism and a detection kit. A complex PCR amplification system comprising three SNP loci and three sample molecular marker sites is firstly designed, the three SNP loci adopt allele-specific primers, the three molecular marker sites adopt length polymorphic primers, and the primers are mutually compatible and can react in the same PCR amplification system. The detection kit comprises a primer composition container, a PCR reaction mother solution container, and a PCR auxiliary solution container, wherein the primer composition container comprises a primer SEQ ID No. 1 to SEQ ID No. 15 composition stock solution. In the case of DNA-free extraction, the three SNP loci are simultaneously amplified and typed by a PCR reaction, so that the cost, manpower and time can be obviously saved, and the working efficiency can be improved.

The invention provides 4-mercaptophenyl boronic acid-modified gold nanoparticles and a method for detecting a sugar marker on cell surface by using the gold nanoparticles. The invention provides a method for detecting a glycometabolism marker and also provides a label close to a natural product, and direct detection for the label is realized on the basis that chemical reaction on the label is not carried out. The detection for sugar on cell surface and labeled by a special Raman signal label is realized by using the nanogold surface enhanced Raman technology under the condition that a toxic catalyst and the like are not needed.

The invention discloses a sensitive, rapid, accurate, high-specificity and economical enzyme immunosensor for detecting Shigella species, comprising screen printing electrodes, a sensitive membrane containing enzyme marked Shigella species antibody- carbon nano-tube- shitosan mixture is coated on the working electrode of the screen printing electrodes. In the invention, the chemical amplification function of the multi-wall carbon nano-tube and enzyme is combined with the specificity of the immunosensor, theenzyme immunosensor has the specificity of immunoreactivity and the sensitivity of the electrochemistry analysis and can accurately detect the lower-content substance. The invention also discloses a preparation method and application of the enzyme immunosensor, the Shigella species can be directly detected, with the sensitive, rapid, high-specificity advantages, enzyme immunosensor is cheap and suitable for detecting the Shigella species in the foundation and in situ.

The invention discloses an NTx (Naltrexone) detection test strip. The NTx detection test strip comprises a chromatography membrane and a conjugate pad; an immunolabelled NTx antibody and an immunolabelled first antibody are loaded on the conjugate pad; the chromatography membrane is provided with an NTx protein covered detection line and a second antibody covered colorimetric line along a liquid chromatography direction. When a sample is detected, the colorimetric line correspondingly displays the chroma of specific protein concentration after the second antibody is specifically combined withthe first antibody; NTx protein on the detection line and the NTx protein in the sample are competitively combined with the immunolabelled NTx antibody and the color developing depth of the detectionline and the colorimetric line is directly compared so that semi-quantitative detection of the NTx protein is realized. The invention discloses a preparation method of the NTx detection test strip; the preparation method is used for preparing an NTx protein semi-quantitative detection test strip which is rapid and sensitive in detection and does not depend on an instrument. The invention disclosesan NTx detection kit; the NTx detection kit comprises the NTx detection test strip and can provide effective information for early diagnosis and treatment of osteoporosis.

The invention provides a method for improving carbon content measuring accuracy in steel, and belongs to the technical field of atomic emission spectroscopy measurement. The method comprises the following steps of puncturing a steel sample by using an ultraviolet band laser to obtain a characteristic spectrum, and fitting a function relationship of the intensities of a carbon atom spectral line and a carbon molecular spectra line and the carbon content; and correcting a residual error of the carbon content after fitting by using a partial least square method so as to obtain a calibration model of the carbon content. The method provided by the invention has the advantages that the ablation efficiency is improved by adopting the ultraviolet band laser, and a measuring signal is effectively strengthened; the information contained in a spectroscopy is fully utilized to establish the calibration model by correcting the residual error, and thus the detection limit of carbon in the steel is reduced and the measuring accuracy is improved.

The invention discloses a screening method and specific primer of superior cow with CD18 gene, which comprises the following steps: adopting detected cow genome DNA as mold; proceeding PCR augumentation for primer couple with nucleotide sequence in the list 1 and list 2; detecting the base at 32th from 5' end as A in the augumentation segment; regarding the cow as normal cow; testing N as heterozygote of A and G.

The invention relates to a method for rapidly identifying true and false pesticides based on portable differential Raman technology. The method adopts a portable differential Raman system, uses two wavelength light sources to detect samples, and comprises the following steps of firstly collecting two pieces of Raman spectrogram data of commercially available pesticides detected by a double-light source Raman spectrometer with stable and tiny wavelength difference, differentiating the two Raman spectra, and performing special algorithm filtering on Raman characteristic signals generated by thedifferentiation to separate the differential signals from baseline deviation, thereby obtaining the differential characteristics of the pesticide samples with eliminated interference of fluorescence and the like; and finally comparing the differential characteristics with the spectrogram characteristic peaks of pesticide standard samples to realize the identification of true and false of commercially available pesticide components. By adopting the double-light source excited differential technology, the sample background interference is eliminated, and more detailed Raman characteristics of the sample are accurately extracted by performing characteristic filtering realization on the differential spectrogram; and a characteristic database and classification identification of the commercially available pesticides are established for the differential Raman characteristics. By using the differential technology, the accuracy and the sensitivity of the detection are greatly improved, the portable Raman system for the detection on the commercially available pesticides is realized, and the detection can be completed without any pretreatment process; and the method is simple in operation, fast in detection speed, high in accuracy and has significance for the pesticide quality safety control.

The invention belongs to the technical field of environment monitoring. The invention relates to a counter, in particular to a two-stage refrigeration particulate matter condensation growth counter. The device comprises an inlet, an evaporation chamber, a steam cooling heat insulator, a condensation chamber section I, a condensation heat insulator, a condensation chamber section II, a nozzle and alight scattering counter which are connected in sequence, an outlet of the light scattering counter is connected with a flow limiting hole through a hose, the flow limiting hole is connected with a three-way connector A through a hose, and a three-way connector B is connected with an air pump through a hose; the pressure measuring device is respectively connected with the three-way connector C, the evaporation chamber and the condensation chamber through hoses; and the temperature measuring device is respectively connected with the evaporation chamber, the condensation chamber I section and the condensation chamber II section through circuits. According to the invention, the flow of particulate matters can be increased, so that the number of the particulate matters passing through the optical detector is increased, the activation area of the particulate matters is enlarged, the detection efficiency of an instrument on small particulate matters is improved, the original series structure of two different working solutions is changed into an independent CPC, and the single-stage detection of 1-3 nano particulate matters is realized.

The invention provides a cascade speed measurement protection method for an escalator. The cascade speed measurement protection method for the escalator mainly comprises the following steps: A. signalacquisition; B. overspeed detection; C. nonmanipulated reversal detection in a running direction; and D. starting and stopping for the escalator. A cascade speed measurement device for the escalatorcomprises a base plate fixedly connected with a fixed frame of the escalator, and two proximity sensors arranged on the base plate, wherein the two proximity sensors are aligned with wheel teeth of two main drive chain wheels which are arranged in parallel separately, and the interval between the two proximity sensors in the circumferential direction of the main drive chain wheels is 5/4 of the tooth distance of the main drive chain wheels. The cascade speed measurement protection method and speed measurement device for the escalator are capable of realizing direct detection for cascade running speed and direction, thereby increasing the safety level.

The invention discloses a detection method and a detection system. The detection method comprises the steps that: a process group at least containing one detection process is established and the data of the wafer to be measured is acquired; the detection process, the detection object of which is the same as that of the wafer to be measured, is selected as a first detection process and the first detection process is adjusted utilizing the data of the wafer to be measured so as to generate a second detection process; the second detection process is executed to accomplish the detection of the wafer to be measured. The adoption of the detection system of the detection method of the invention can set the steps and conditions for detection under the circumstance that the wafer is not provided, thereby avoiding the process flow from being stopped due to the setting of the detection process and enhancing the production efficiency.

The invention discloses a sensor for measuring a salt content on the surface of a solid. The sensor comprises water-absorbing test paper, an anisotropic conductive film, a conductance cell and an insulating hydrophobic isolating device, wherein the water-absorbing test paper is bonded on an electrode of the conductance cell through the anisotropic conductive film; the anisotropic conductive film is electrically conducted in a direction perpendicular to the water-absorbing test paper and is insulated in a direction parallel to the water-absorbing test paper; the electrode of the conductance cell is electrically connected with an external conductivity measuring device; the insulating hydrophobic isolating device is used for preventing liquid in the water-absorbing test paper from diffusing to the external conductivity measuring device. By using the sensor, the defects that a to-be-detected sample needs to be sampled and detected (for example, a mural can be detected only after being subjected to micro core sampling) in the prior art are overcome; the additional sampling is not needed; the direct detection on the salt content on the fixed surface of the to-be-detected sample is realized; the destroy cannot be caused to the to-be-detected sample.

The invention discloses a florfenicol and florfenicol amine antigen, antibody and a simultaneous detection enzyme-linked immuno sorbent assay analytical method thereof. The simultaneous detection enzyme-linked immuno sorbent assay analytical method utilizes florfenicol as a hapten to be coupled with a carrier protein to obtain an artificial antigen, and the artificial antigen is used as a coatingantigen; meanwhile, the hydroxyl group of the florfenicol molecule derives the succinic acid half ester to couple with arm to obtain the florfenicol hapten, and the florfenicol artificial antigen is obtained as an immunogen by coupling with the carrier protein; the detection antibody is prepared, and the enzyme-linked immuno sorbent assay analytical method is established. The IC50 of florfenicol is 37.10ng/mL, the linear detection range is 12.03-114.49ng/mL, the IC50 of florfenicol amine is 45.3ng/mL, and the linear detection range is 10.76-190.69ng/mL. The method can be used for quickly detecting the residual florfenicol and florfenicol amine in the food, and has wide application prospect.

The invention belongs to the technical field of biomedicine, and discloses a nucleic acid aptamer specifically bound with vomitoxin, a preparation method and an application. The nucleic acid aptamer is ssDNA and composed of 82 nucleotides, wherein the nucleotide sequence thereof is shown as SEQ ID NO:1. The secondary structure of the nucleic acid aptamer contains protruding rings and stems, wherein DONA15 Gibbs free energy DG is equal to -8.84. The aptamer has a G-quadruplex structure which is more stable. Based on an enzyme-linked oligonucleotide adsorption determination method (ELONA), a series of property evaluations such as specificity, affinity and sensitivity of the nucleic acid aptamer are carried out, and the result shows that DONA15 can be specifically bound with vomitoxin. The nucleic acid aptamer has the characteristics of high specificity and high affinity, and can be applied to subsequent rapid detection of vomitoxin in a test strip.

The present invention provides an electric kettle. A detection circuit in the electric kettle is used to detect at least one of the following items: the voltage of a first end of a heating circuit, a current flowing through the heating circuit and the power of the heating circuit, and a control circuit is used to adjust a control signal sent to a switching circuit when the deviation of at least one detection result of the detection circuit and a preset numerical value corresponding to the detection result is not within a preset range, and determine the water yield in the electric kettle after the situation that the deviation of the detection result and the corresponding preset numerical value is within the preset range is detected for the first time, and according to the detection temperatures detected by a temperature sensor at at least two moments. By adding the detection circuit in the electric kettle, detecting and controlling the voltage, current or power of the heating circuit, the electric kettle works under a stable power, and further the water yield in the electric kettle is determined according to the temperature change within the fixed time period, thereby improving the water yield detection accuracy.

The invention discloses a sieving method of hesitant cow BLAD carrier and specific primer and agent box, wherein the primer is composed of primer couple with nucleic acid sequence in the list 1 and list 2. The sieving method comprises the following steps: adopting detected cow genome DNA as mold; proceeding PCR augumentation guided by primer; recycling PCR augumented segment; using restriction enzyme TaqI to cut the segment; detecting the segment at 193bp and 131bp as normal cow and 324bp, 193bp and 131bp DNA as BLAD carrier and 324bp DNA as BLAD ill cow; fitting for selecting superior cow and detected one with BLAD gene effectively.

The invention relates to the technical field of optical communication, in particular to a coherent light direct detection method and device and an optical communication system. According to the coherent light direct detection method of the invention, modulated signals are separated, so that four paths of component light signals can be obtained; photoelectric conversion is carried out according tothe four paths of component optical signals to obtain electric signals corresponding to the four paths of component optical signals; and detection is carried out according to the electric signals corresponding to the four paths of component optical signals to obtain light intensity corresponding to the four paths of component optical signals, calculation is carried out according to the light intensity corresponding to the four paths of component optical signals to obtain Stokes parameters corresponding to modulated signals, and the Stokes parameters corresponding to the modulated signals describe the polarization state of the modulated signals. According to the invention, the polarization state of the modulated signal is detected by adopting a non-coherent method through high-integration design, so that the direct detection of a coherent communication system is realized, the polarization demodulation is satisfied, the complexity is reduced, and the cost is reduced.

The invention discloses a monoclonal antibody capable of specifically identifying tenuazonic acid and its enzyme-linked immunosorbent assay method. The monoclonal antibody of tenuazonic acid is prepared by firstly preparing hapten (TEA-CMO), and then respectively coupling hemocyanin (KLH) and bovine serum albumin (BSA) to obtain immunogen (TEA-CMO-KLH) and package (TEA-CMO-BSA); then applying animal immunization and hybridoma technique to obtain the monoclonal antibody of tenuazonic acid; an indirect competition enzyme-linked immunosorbent assay method built by the monoclonal antibody of tenuazonic acid is a rapid detecting method of tenuazonic acid residue; the detection limit (IC10) of the method is 1.00 ng/mL, IC50=18.50 ng/mL; the linear detection scale is 3.56-96.24 ng/mL; the direct detection of the tenuazonic acid can be realized; the monoclonal antibody is free from crossing reaction with similar matters of another nine alternaria alternata aflatoxin structures; the method is good in specificity and high in sensitivity.

The invention discloses a fluorescent ring-mediated isothermal amplification method based on a molecular beacon. The amplification method introduces a molecular beacon into the LAMP technology, wherein the fluorescent ring-mediated isothermal amplification method based on the molecular beacon is established and named as MB-LAMP, thereby realizing direct detection of amplified products, and fundamentally solving the problem of non-specific amplification of the LAMP method, great application value and commercial value are achieved.

The invention discloses a method for measuring N-acetylglucosamine by utilizing ion exchange chromatography. The method comprises the following steps: on an ion exchange chromatography, detecting by adopting a DionexAminPacPA10 chromatographic column with the temperature of 30 DEG C, taking 10mmol/L of sodium hydroxide as a mobile phase and using an electrical conductivity detector; drawing a concentration-peak area standard curve of the N-acetylglucosamine by adopting an external standard method; calculating to obtain the content of the N-acetylglucosamine. A method for accurately and quantitatively measuring the N-acetylglucosamine through high performance ion exchange chromatography is established at first, the DionexAminPacPA10 chromatographic column is adopted, and the separation degree and detection efficiency of the N-acetylglucosamine are obviously improved through isocratic elution of the 10mmol/L of sodium hydroxide. The method is high in precision, high in reproducibility and high in accuracy and can realize direct sample detection, the sample is not needed to be subjected to other pretreatment, the analysis time is greatly reduced, the accuracy, convenience and reliability of the detection method are improved, and the method has significance for detection, evaluation, popularization and application of N-acetylglucosamine products.