100 results about "Desoxyribonucleotide" patented technology

The invention provides an anti-aging repair composition containing PDRN, and lipid nanoparticles and application thereof. The composition provided by the invention is prepared from polydeoxyribonucleotide, micro-current tetrapeptide-1 and palmitoyl tripeptide-5. According to the application, the polydeoxyribonucleotide, the micro-current tetrapeptide-1 and the palmitoyl tripeptide-5 are compounded for use, so that the composition has a synergistic effect in the aspects of promoting cell proliferation, inhibiting an intracellular ROS (reactive oxygen species) level and the like, and has remarkable antioxidant, anti-inflammatory, anti-aging, soothing and repairing effects. The PDRN-containing composition is prepared into the lipid nanoparticles, so that the stability of the PDRN-containing composition is improved, the permeability of the PDRN-containing composition on the skin surface is improved, the bioavailability of the PDRN-containing composition is improved, the cell survival rate and cell uptake are promoted, the expression of adenosine A2A receptors, the synthesis of collagen, angiogenesis and the inhibition of anti-inflammatory factors are promoted, and a remarkable anti-aging effect is achieved.

The invention relates to a single nucleotide polymorphism (SNP) primer of a DSTN gene related to musk ketone content in natural musk and application of the single nucleotide polymorphism (SNP) primer, and belongs to the technical field of biological detection. The invention provides a single nucleotide polymorphism (SNP) primer of a DSTN gene related to the musk ketone content in natural musk, a molecular marker corresponding to the SNP primer is located at a 682371th basic group of a chromosome 23 of a forest musk deer reference genome, the basic group is mutated into A or C, the genotypes are AA and AC, and deoxyribonucleotide sequences of the SNP primer are shown as SEQ ID NO: 1 and SEQ ID NO: 2. According to a breeding target, by eliminating A / A genotype individuals and retaining A / C genotype individuals, the method has the beneficial effects that the relative content character of musk ketone in musk of the forest musk deer is efficiently and quickly identified, and a scientific basis is provided for early breeding of high-quality forest musk deer. The detection method disclosed by the invention is simple and easy to operate, can be carried out in a laboratory, and can also be applied to a genome breeding technology.

The invention discloses a lipophilic liposome containing polydeoxyribonucleotide as well as a preparation method and application of the lipophilic liposome. The lipophilic liposome comprises the following components in parts by weight: 0.001-3 parts of polydeoxyribonucleotide; 0.5 to 3 parts of phospholipid; 0.1-2 parts of a cationic emulsifier; 0.1-3 parts of an anionic emulsifier; 40 to 70 parts of polyol; the total amount is 100 parts. According to the invention, an emulsifier system formed by combining lecithin with anionic and cationic compound surfactants is adopted, so that the transdermal absorption of PDRN is facilitated. The lipophilic liposome containing polydeoxyribonucleotide prepared by the invention can effectively resist nuclease degradation, prolong the acting time of PDRN in skin, and improve the skin anti-aging and repairing effects of the liposome. The anti-aging effect of the active ingredients is improved, and the bioavailability of the active ingredients is improved. The cosmetic composition can be widely applied to cosmetic formulas of various dosage forms.

The invention relates to an SLC26A11 gene SNP (Single Nucleotide Polymorphism) primer related to androstenediol content in forest musk and application, and belongs to the technical field of biology. The invention provides an SLC26A11 gene SNP primer related to androstenediol content in moschus berezovskii, a molecular marker corresponding to the SNP primer is located at the 55966787 base of chromosome 21 of a moschus berezovskii reference genome, the base is mutated into A or G, the genotypes are AG and GG, and deoxyribonucleotide sequences of the SNP primer are shown as SEQ ID NO: 1 and SEQ ID NO: 2. The detection method disclosed by the invention is simple and easy to operate, can be carried out in a laboratory, and can also be applied to a genome breeding technology. The method has the beneficial effects that the relative content character of androstenediol in the forest musk deer can be efficiently and quickly identified, and a scientific basis is provided for early breeding of high-quality forest musk deer.

The invention provides a polyethylene glycol derivative-collagen cartilage repair gel which is characterized by being prepared from the following components in parts by weight: 8 to 12 parts of collagen (namely COL) solution, 0.5 to 2 parts of polydeoxyribonucleotide (namely PDRN), 4 to 8 parts of polyethylene glycol disuccinimide succinate (namely SS-PEG-SS) solution and 0.1 to 0.4 part of tannic acid (namely TA), and particularly discloses a preparation method and application of the polyethylene glycol derivative-collagen cartilage repair gel. In a word, the composite gel and the collagen are subjected to efficient amidation reaction through SS-PEG-SS to form a stable three-dimensional network, so that the structural support of the type I collagen, the tissue repair of PDRN and the anti-inflammatory and anti-oxidation characteristics of tannic acid are successfully integrated; therefore, the composite material has the comprehensive performance of good structural stability, high mechanical strength, good biocompatibility, controllable degradation period, excellent needle passing performance and the like, and shows the wide application prospects of clear repair effect, remarkable component synergistic effect, mature production process, outstanding cost effectiveness and the like.

The invention discloses extraction of polydeoxyribonucleotide as well as preparation and application of a PDRN-Ce (Polydeoxyribonucleotide-Ce) microsphere cluster. The extraction and purification method comprises the following steps: mixing in-vitro fish testis tissues with water, carrying out crushing treatment and homogenization treatment on an obtained premix, carrying out proteolysis and solid-liquid separation, and collecting supernate to obtain enzymatic hydrolysate; wherein protease used for proteolysis comprises neutral protease; carrying out alcohol precipitation on the enzymatic hydrolysate by adopting an alcohol solvent, and collecting a precipitate; and dissolving the precipitate in water, desalting and drying. According to the extraction method, the purity and the yield of the poly-deoxyribonucleotide are remarkably improved, the preparation method is low in cost and convenient to operate, the prepared poly-deoxyribonucleotide is stable in property, small in molecular weight and easy to absorb by skin, in order to further improve the bioavailability of the extracted poly-deoxyribonucleotide, the PDRN-Ce microsphere cluster is prepared, and the PDRN-Ce microsphere cluster can be used for preparing the poly-deoxyribonucleotide. Good application prospects are realized.

The invention discloses a preparation method and application of polydeoxyribonucleotide derived from prokaryotes. The preparation method comprises the following steps: 1) enriching blue-green algae biomass; 2) ultrasonically crushing; (3) carrying out RNA elution; 4) DNA and protein separation; (5) dissolving the DNA; (6) separating out DNA; 7) gradient cleaning of DNA; (8) dissolving the DNA; and (9) preparing PDRN. The preparation method of the water-blooming cyanobacteria PDRN is developed by taking the prokaryote cyanobacteria as the raw material, the preparation method is simple and easy to implement, the molecular weight of the PDRN can be efficiently controlled, the purity and yield of DNA are ensured by controlling the RNA elution step and the DNA and protein separation step, the antioxidant capacity of the DNA is verified, and the method is suitable for industrial production. And a new way is provided for resource utilization of the water-blooming cyanobacteria biomass.

The invention provides a preparation method and application of a high-activity salmon salmon sperm PDRN (DNA sodium) raw material. The preparation method comprises the following steps: crushing and freezing a salmon testis at an ultralow temperature, adding a mixed solution of a Tris-HCl buffer solution and sodium chloride, and dispersing at a low temperature; adding a compound enzyme preparation with the total enzyme activity of more than or equal to 350KU / L, and carrying out low-temperature appropriate pH enzymolysis; and adjusting the pH value to a suitable range, and carrying out frequency conversion ultrasonic and high-pressure homogenization alternate synergistic treatment in an ice-water bath to obtain the DNA fragmentation liquid, so that the defects of low PDRN activity retention rate, insufficient purity and high protein residue in the prior art are overcome.

The invention provides a repairing composition and application thereof. The repairing composition at least comprises the following components: (1) polydeoxyribonucleotide; (2) gamma-polyglutamic acid or a salt thereof; and (3) hyaluronic acid or salts thereof, and the three components of polydeoxyribonucleotide, gamma-polyglutamic acid or salts thereof and hyaluronic acid or salts thereof in the repairing composition are compounded to achieve a synergistic effect, so that the repairing composition is beneficial to improving skin sensitivity, repairing skin barriers, stimulating collagen regeneration and the like.

The invention relates to a poly (deoxyribonucleotide) self-assembled supramolecular nanoparticle composition, a preparation method and application thereof and cosmetics, the poly (deoxyribonucleotide) self-assembled supramolecular nanoparticle composition comprises PDRN and mussel protein, and the mass ratio of the PDRN to the mussel protein is 1: (0.05-10). The PDRN-mussel protein self-assembled supramolecular nano-particles have the beneficial effects that the PDRN-mussel protein self-assembled supramolecular nano-particles are prepared by utilizing the inherent charge properties of PDRN and mussel protein, so that the microscopic sizes of the PDRN and mussel protein are compressed, the transdermal absorption of the PDRN and mussel protein is greatly improved by utilizing the properties of the nano-particles, and a synergistic repairing effect is shown.

The invention discloses a poly-deoxyribonucleotide salt and a preparation method and application thereof, the content of the poly-deoxyribonucleotide salt is greater than 95%, the protein content is less than 1.0%, and after high-pressure moist heat sterilization, the dynamic viscosity reduction amount does not exceed 25%, and the elastic index G'reduction amount does not exceed 30%. The polydeoxyribonucleotide salt disclosed by the invention is simple in preparation process, short in production period, low in cost and suitable for large-scale industrial production; and the preparation process is mild, few impurities are introduced, the product structure is less damaged, and the preparation method is environment-friendly. The polydeoxyribonucleotide salt disclosed by the invention has good physical support performance, has the effects of promoting skin cell repair, resisting skin sensitivity, whitening, resisting inflammation, preventing alopecia and growing hair, and has a wide market prospect.

The present invention discloses a molecular marker tightly linked to the light green trait of green ripe pepper fruit and its application. The present invention provides the application of a substance for detecting whether the deoxyribonucleotide at position 174795925 on chromosome 10 in the pepper genome is A, G, or both A and G for the following purposes: identifying the color trait of green ripe pepper fruit; identifying the depth of the peel color of green ripe pepper fruit. The dCAPS10-1 marker developed by the present invention had a phenotypic matching rate of 95.92% in the constructed F2 population. In addition, the marker dCAPS10-1 was used to perform genotyping on 126 pepper materials with known green ripe fruit colors stored in the laboratory. The results showed that the success rate of verification of the 126 inbred line materials using the dCAPS10-1 marker was 76.20%, of which the phenotypic matching rate was 79.37% for 63 materials with light green green ripe fruit and 73.00% for 63 materials with green green ripe fruit, indicating that this marker has strong applicability and can be used for rapid identification of green ripe fruit color in practical breeding applications.

The invention discloses a method for distinguishing clematis palustris from other clematis plants and application of the method. The method for distinguishing the clematis from the other clematis plants comprises the following steps: detecting whether the genotype of 201st deoxyribonucleotide in a sequence 1 of a plant to be detected is an AA genotype or a CC genotype by adopting a primer pair consisting of a single-stranded DNA (Deoxyribonucleic Acid) shown in a sequence 2 and a single-stranded DNA shown in a sequence 3; the to-be-detected plant is clematis hispida; if the genotype of the plant to be detected is AA genotype, the plant to be detected is other clematis plants. Experiments prove that the method provided by the invention can be used for effectively distinguishing the clematis palustris from other 14 clematis plants, and an important tool is provided for quickly and accurately identifying the clematis palustris in the seedling stage.

The present disclosure relates to a method of inhibiting nuclease activity in a biological sample. In accordance with certain embodiments of the present disclosure, the method comprises mixing a biological sample with a metal-chelating agent complex. The present disclosure also discloses a method of isolating a cell nucleus from a cell in a biological sample by using the metal-chelating agent complex, and a method of adding a deoxyribonucleotide to the 3'terminal of a deoxyribonucleic acid.

The invention relates to the technical field of medical preparations, in particular to moisturizing and repairing type artificial tear as well as a preparation method and application thereof. Comprising the following steps: preparing effective components including high-molecular-weight polydeoxyribonucleotide and hyaluronic acid or a salt thereof into a homogeneous-phase stable composite solution, sterilizing, filtering, and aseptically packaging, the artificial tear formed by selecting high-molecular-weight polydeoxyribonucleotide and high-molecular-weight sodium hyaluronate as effective components has a relatively good repairing effect on tissues, can form long-term moisturizing and lubrication on ocular surfaces, is suitable for treating xerophthalmia, repairing cornea, relieving inflammatory response and the like, and has a good application prospect. Important market values and clinical application prospects are realized.

The invention discloses hydrogel for repairing osteoarthritis as well as a preparation method and application of the hydrogel, and relates to the technical field of biological medicines. The hydrogel is prepared from polydeoxyribonucleotide and polyglutamic acid, the hydrogel has a porous structure, and the average pore size is 50-200 [mu] m. The preparation method comprises the following steps: mixing a PDRN solution with a PGA solution, then adding a cross-linking agent solution, uniformly stirring and mixing, and standing for reaction to form the hydrogel. The mechanical strength of the hydrogel can be improved after PDRN and PGA are crosslinked, meanwhile, PDRN can be slowly released in the hydrogel, the anti-inflammatory effect is achieved, and the obvious treatment effect on osteoarthritis is achieved.

Provided is a method for detecting a target nucleic acid by cleaving a non-natural sequence using a Cas12 protein, belonging to the technical field of biology. The Cas12 protein belongs to a Cas12a / Cas12b protein family. The non-natural sequence includes a chimeric sequence composed of a ribonucleotide and a deoxyribonucleotide. Such a sequence can be made into a probe for nucleic acid detection, and its detection effect is better than that of a conventional ssRNA probe, and in certain cases is equivalent to or even better than that of an ssDNA probe. Also provided is a system for detecting a target nucleic acid, including a chimeric sequence, a Cas12 protein and crRNA. The pre-amplification can increase the detection limit of the system to a single-molecule level. The Cas12-chimeric sequence detection system can be used not only on a microfluidic chip, but also in clinical testing. In summary, the coordinated use of the Cas12 protein and chimeric sequence optimizes the CRISPR / Cas12 detection system and expands the use of the Cas12a protein and non-natural sequence.

An aptamer composition is disclosed which has one or more oligonucleotides that include at least one of deoxyribonucleotides, ribonucleotides, derivatives of deoxyribonucleotides, derivatives of ribonucleotides, or mixtures thereof. The aptamer composition has a binding affinity for one or more cellular membrane glycoproteins selected from the group consisting of: intercellular adhesion molecule 1 (ICAM-1), low-density lipoprotein receptor (LDLR) family members, and cadherin-related family member 3 (CDHR3), preferably intercellular adhesion molecule 1 (ICAM-1), and is configured to reduce the binding of one or more human rhinoviruses to the intercellular adhesion molecule 1 (ICAM-1).

The present application relates to siRNA for inhibiting ACVR2A and application thereof, and belongs to the technical field of biotechnology.The present application provides siRNA for inhibiting ACVR2A gene expression, research shows that the siRNA all has high inhibitory activity to the expression of ACVR2A gene and protein.The present application also provides modified siRNA for inhibiting ACVR2A gene expression, the modification includes 2'-methoxy modification, 2'-fluorine modification, phosphorothioate group connection, reverse baseless nucleotide modification, deoxyribonucleotide substitution and / or vinyl phosphate modification, research shows that the modified siRNA all has good ACVR2A inhibitory effect at the concentration of 1nM-10nM.

The application discloses a KASP marker related to pea powdery mildew resistance and application thereof. The application also discloses a SNP site related to pea powdery mildew resistance, which is the 583th deoxyribonucleotide of the DNA molecule described in sequence 4 in the sequence listing in the pea genome, and the polymorphism is T / -; the application further discloses a KASP marker developed based on the SNP site for identifying the pea powdery mildew resistance. Experiments prove that the KASP marker can be effectively applied to identification of pea powdery mildew resistance resources and molecular marker assisted breeding of a pea powdery mildew resistance offspring population, and has important value in pea production, breeding work and research on disease resistance mechanism.

The application provides a kind of self-assembly based biological gel and its preparation method and application.The biological gel comprises palmitoyl pentapeptide-4, polydeoxyribonucleotide and chitosan, wherein the polydeoxyribonucleotide is encapsulated in the self-assembly structure of palmitoyl pentapeptide-4, the chitosan is coated outside the self-assembly structure by electrostatic interaction, and the mass ratio of palmitoyl pentapeptide-4 to polydeoxyribonucleotide is 1.5-30:1.The combination of palmitoyl pentapeptide-4, PDRN and chitosan improves the bioavailability and transdermal absorption of active substances and produces synergistic anti-aging effect, greatly improving the anti-aging effect.

The present invention relates to nucleic acid fragment-encapsulated polymer nanoparticles and a method of preparing the same. The polymer may be a mucoadhesive polymer and specifically chitosan, and the nucleic acid fragment may be polydeoxyribonucleotide (PDRN). The nucleic acid fragment-encapsulated polymer nanoparticles may be used as an eye drop. Since the nucleic acid fragment-encapsulated polymer nanoparticles is capable of adhering to the mucus in the eyes, nucleic acid fragments inside the nanoparticles can be slowly released into the ocular mucous membrane. In addition, since nucleic acid fragment-encapsulated polymer nanoparticles can exhibit high efficiency in the eyes even with a small dose of drug, patient convenience can be increased or costs can be reduced.

The invention provides a skin repair composition, a skin repair product and application of the skin repair composition and the skin repair product. The skin repair composition comprises the polydeoxyribonucleotide and the salt thereof, the Ectoine or the derivative thereof and the schizophyllan, overcomes the defect of single function of bioactive substances, and has the effects of inhibiting expression of inflammatory factors and accelerating repair of damaged skin pigmentation. The three active ingredients in the skin repair composition are not simply superposed, but generate a synergistic gain effect of '1 + 1 + 1gt, 3', the safety and effectiveness are obviously superior to those in the prior art, and a brand-new and excellent solution is provided for solving the problems of light and moderate acne of teenagers and hyperpigmentation after healing.

The present application relates to the technical field of cosmetic biotechnology and nano-carrier drug delivery, and provides PDRN-putrescine co-delivery nano-liposome as well as a preparation method and application thereof.The PDRN-putrescine co-delivery nano-liposome provided by the present application comprises the following components in mass fraction: 0.1-3% of polydeoxyribonucleotide; 0.1-3% of putrescine; 2-8% of phospholipid emulsifier; 0-15% of polyhydric alcohol cosolvent; and the balance is a buffer solution; and the buffer solution has a pH value of 6-7.5.The present application realizes stable co-encapsulation of PDRN and putrescine by constructing a specific buffer system to regulate the pH value, using the amphiphilic property of the phospholipid bilayer and the in-situ ion pair technology, solves the compatibility problem of PDRN and putrescine, significantly improves the transdermal absorption rate of PDRN through transmembrane delivery of the nano-carrier, and realizes the synergistic repair and anti-aging effect of PDRN and putrescine at the cellular level.

The application discloses a primer combination and a kit for detecting Chlamydophila psittaci based on an isothermal amplification method. The primer combination comprises a specific isothermal amplification primer and a gRNA sequence. The kit comprises a reaction buffer, an enzyme mix, and a primer probe mix. The application also provides a method for detecting Chlamydophila psittaci by using the kit. The method can be used for isothermal amplification detection of deoxyribonucleotides in a sample to be detected. The detection method has low requirements for temperature control, is simple to operate, and can reduce the requirements for laboratory equipment. The amplification and detection are synchronously performed, and the whole reaction can be completed within 30 minutes. The detection result has good specificity and high sensitivity. The whole experiment process does not need to be opened, and the risk of laboratory aerosol pollution is greatly reduced. The kit has the advantages of simple operation, good specificity and high sensitivity, and has great clinical significance in the detection of Chlamydophila psittaci.

The invention belongs to the technical field of biosynthesis, and relates to a terminal deoxyribonucleotide transferase mutant, a preparation method and a kit. The mutant is obtained by carrying out multipoint mutation on a truncated wild type terminal deoxyribonucleotide transferase amino acid sequence, and mutation sites comprise R336L, K338G, L397M and E456S or E456G. Compared with the prior art, the method disclosed by the invention has the advantages that the time for rationally transforming the wild type BtTdT is shorter, the transformation efficiency is high, the catalytic efficiency of the obtained mutant is obviously improved, and the mutant has the best activity for catalyzing 3 '-ONH2-dNTP reported at present; and the transformation method of the mutant can be applied to transformation of other TdTs so as to be suitable for improving the catalytic efficiency of the TdTs on other 3 '-block substrates.

The invention discloses an SNP (Single Nucleotide Polymorphism) site for distinguishing Clematis brevicaulis from other Clematis plants and application of the SNP site. The name of the SNP site is CLA25318-11, the SNP site is located at the 305th deoxyribonucleotide of SEQ ID NO: 1, the polymorphism of the SNP site is C / A, and the SNP site has two genotypes: CC and AA genotypes. The invention also discloses a PCR (Polymerase Chain Reaction) primer pair for detecting the genotype of the SNP locus. By adopting the PCR primer pair to carry out PCR amplification on genomic DNA of 15 clematis plants, it is found that only the short-column clematis is of a CC genotype, and the other clematis plants are of an AA genotype. Therefore, the clematis brevituba and other 14 clematis plants can be effectively distinguished by utilizing the SNP site provided by the invention. The invention provides a powerful tool for early accurate identification of the clematis brevicaulis and systematic taxonomic status research of the clematis brevicaulis.

The application discloses a SNP marker related to cold resistance on the short arm of wheat 2A chromosome and application thereof, and belongs to the field of biotechnology and molecular breeding. In order to identify, assist in identifying and / or compare the cold resistance of wheat, the application provides application of a SNP molecular marker, wherein the SNP molecular marker is a deoxyribonucleotide at position 361 of SEQ ID NO:1, which is G or A, and the application can be application of the SNP molecular marker in identifying or assisting in identifying the cold resistance of wheat. By using whole genome association analysis, the application identifies that a SNP at position 361 of the nucleotide sequence of SEQ ID NO.1 is significantly related to the cold resistance of wheat, the SNP molecular marker is not limited by the growth stage of wheat, and selection can be performed at the seedling stage, so that the selection period of wheat breeding is significantly shortened, the breeding efficiency is improved, and the SNP molecular marker assisted breeding for improving the cold resistance of wheat can be used in practice.

The present invention relates to a cosmetic composition for promoting skin regeneration, and more particularly, to a cosmetic composition for promoting skin regeneration by further comprising a polydeoxyribonucleotide (PDRN) in a composition comprising a sponge microneedle, a lactic acid bacteria exosome, and a ceramide in a specific weight ratio, and more particularly, to a cosmetic composition for promoting skin regeneration by adding a polydeoxyribonucleotide (PDRN) to a composition comprising a sponge microneedle, a lactic acid bacteria exosome, and a ceramide in a specific weight ratio. The sponge microneedle forms a microchannel in a skin cuticle to promote transmission of effective components, the lactic acid bacteria exosome induces cell viability through a cell signal transduction function, and the ceramide strengthens a skin barrier. By adding PDRN in the range of 0.001 to 3% by weight relative to the total weight of the composition, proliferation of skin fibroblasts, collagen synthesis and wound healing can be promoted. The composition can be prepared into various dosage forms such as face cream, emulsion, essence, ampoules or facial masks, and the improved skin regeneration effect is provided by simultaneously achieving the effects of strengthening the skin barrier, activating cells and promoting tissue regeneration.

This invention belongs to the field of agricultural biotechnology and discloses a low-temperature synergistic biological agent, its preparation method, and its application. The core innovation lies in using a unique engineered Bacillus subtilis strain as the core functional carrier, and performing a ternary targeted compounding with polydeoxyribonucleotides (PDRN) and biostimulants to achieve a triple synergistic low-temperature resistance effect. The engineered Bacillus subtilis strain was deposited at the China General Microbiological Culture Collection Center (CGMCC) on March 11, 2026, with accession number CGMCC No. 37899. The chassis strain of Bacillus subtilis was deposited at the same center on March 3, 2026, with accession number CGMCC No. 37834. The modification strategy involves knocking out the α-subunit gene of acetoin dehydrogenase and the phosphoacetyltransferase gene from the genome of Bacillus subtilis CGMCC No. 37834, and expressing the acetyllactate synthase gene and the α-acetyllactate decarboxylase gene.