90 results about "Desoxyribonucleotide" patented technology

A nucleic acid, which is provided in a large amount through a nucleic acid amplification reaction with the use of chimeric oligonucleotide primers, is constructed in a state of containing a modified deoxyribonucleotide for immobilizing the nucleic acid to a solid phase and then immobilized to a solid phase at a high efficiency, thereby giving an immobilized nucleic acid product with excellent qualities.

The invention discloses a specific promoter of a root and a recombinant expression vector. The promoter adopts DNA molecular: a of following a) or b) or c) DNA molecular: a) is a DNA molecular which is composed of the deoxyribonucleotide shown in the sequence 1 in the sequence table; b) is a DNA molecular hybridized with a) defined DNA sequence under the strict condition; and c) is a specific expression DNA molecular in the root of the plant and has over 90 percents of homology and the gene with a adjusting and controlling purpose with a) defined DNA sequence. The invention also discloses a recombinant expression vector with the promoter, and the recombinant expression vector can be applied for cultivating the transgenic plant with a specific expressing target gene in the plant root.

The invention provides a salmon small molecule polydeoxyribonucleotide (SMPDRN) product with a molecular weight concentrated in an efficacy range of the salmon small molecule polydeoxyribonucleotide product, a controllable precise preparation method of the salmon small molecule polydeoxyribonucleotide product, and application of the salmon small molecule polydeoxyribonucleotide product to the fields of cosmetics, medicine, nutritious food and health food. The molecular weight of small molecule polydeoxyribonucleotide is 50-1000 bp, and the mass of the molecular weight of the small molecule polydeoxyribonucleotide at the 50-500 bp fragment accounts for more than 85% of the total mass of the small molecule polydeoxyribonucleotide; and the molecular weight of the SMPDRN is concentrated in thehigh-efficacy range, the pertinence is higher, the efficacy is more stable, and the obtained product has significant effects in improving cell activity, promoting collagen synthesis, regenerating damaged skin, promoting wound healing, tightening skin, increasing skin elasticity, eliminating wrinkles, delaying skin aging, resisting oxidation, preventing formation of color spots, resisting inflammation, repairing damaged cells and other aspects by being verified by cell, animal and human experiment, and is better than an existing PDRN product.

The invention discloses an SNP label relevant to root characteristics in a wheat seedling stage and application. The invention provides a genotypic substance of 19-site deoxyribonucleotide of AX-110602569-7A gene in a detected wheat genome provided in the invention in application of authentication or auxiliary authentication for wheat root characteristics, and the 39cM position of the AX-110602569-7A gene in wheat chromosome 7AS. The invention provides a rated site QRL.caas-7A for root characteristics in the wheat seedling stage and the SNP site of the site for auxiliary sieving of the rated genes in the root system in the wheat seedling stage. Wheat with excellent related root characteristics can be sieved by using the SNP site, and the SNP plays an important role in cultivating water andfertilizer high-efficiency wheat varieties.

The invention provides a reverse transcription obstruction probe for rapidly removing target RNA in RNA library construction and application thereof. The probe has a length of 20-40 nt, is strictly complementarily paired with a target RNA sequence, and has no complementary pairing continuously exceeding 15 nt with other non-target RNAs, the Tm value of the probe is not lower than 80 DEG C, the self complementary value is less than 5, and 3'-OH of the probe is sealed by modified deoxyribonucleotide. According to the probe, a reverse transcription inhibition probe method is utilized to realize high-efficiency and high-specificity inhibition on reverse transcription of the target RNA during synthesis of one strand of cDNA, so that the target RNA is prevented from participating in a downstream library construction process. The probe has the advantages of simplicity in operation (one-step operation), extremely short consumed time (2 minutes), completeness in retention of the non-target RNAs, high specificity, high efficiency, low cost, high gene detection number and the like, and is very suitable for the fields of industrial automatic library building and rapid disease diagnosis.

The invention discloses a method and a kit for detecting the correlated mutation allele of a total farrow number of a sow as well as the application. The method adopts a PCR (Polymerase Chain Reaction)-SSCP (Single-Strand Conformation Polymorphism) method, PCR augmentation comprises a section from 315-bit deoxyribonucleotide at the 5' terminal of Gen Bank Accession Number AX752829, PCR augmentation products are detected by gel electrophoresis, and the gene type of the sow to be detected is confirmed according to an electrophoresis result: the gene type of the sow to be detected can be GG or AA which means a homozygote if the electrophoresis result presents two straps, and the gene type of the sow to be detected can be GA which means a heterozygote if the electrophoresis result presents three straps. The detection method of the invention has simple operation, low expense and high accuracy, can realize the automatic direct detection and has higher practical breeding application values. The method and the kit of the invention can be applied to breed grices, early select grices to be selected, effectively solve the problem of long time for selecting excellent grices in practical production, decrease the breeding expense, increase the total sow farrow number and improve the economic benefits.

The invention discloses a method for assisting the judgment of a Wuzhishan minipig inbred line. The method for assisting the judgment of the Wuzhishan minipig inbred line and/or a non-Wuzhishan-mountain minipig inbred line, which is provided by the invention, detects that the 1400th position of deoxyribonucleotide from the 5' end of GenBankAccession Number DQ406743 of a pig to be detected is C or T, and determines that the genotype of the pig to be detected is CC, CT or TT; the pig to be detected with the CC genotype is an alternate Wuzhishan minipig inbred line; and a pig with the CT genotype and a pig to be detected with the TT genotype are non-Wuzhishan-minipig inbred lines. The method can be applied to the seed breeding of the Wuzhishan minipig inbred line. The method can firstly carry out preliminary screening on all the pigs in a swinery to be detected, eliminates the non-Wuzhishan-minipig inbred line, finds out the alternate Wuzhishan minipig inbred line and combines with other methods to carry out further validation. The invention can also be used for detecting whether the Wuzhishan minipig inbred line purchased in the market is counterfeit or not.

The invention discloses a kit for single cell genome-wide amplification and application thereof. The invention protects a kit for single cell genome amplification first, comprising a second amplification system; the second amplification system is composed of 10*Phi29buffer, water and a first amplification system; the first single amplification system is composed of dNTP (1.8-2.2)*10-8 mol, N8 primers (1.8-2.2)*10-11 mol in total concentration, BSA (4.5-5.5)*10-3 mg, Pluronic F68 0.0045-0.0055Mul, and DNA polymerase 9-11 U Phi29; the N8 primers are random primers, composed of eight deoxyribonucleotides, in a random arrangement of A, G, C and T. The WGA kit low in cost, high in efficiency and stable in amplification effect is developed based on the multiple exchange amplification principle and amplification features of DNA polymerase Phi29, and the kit has an important applicable prospect and polarization value.

Improved processes for the amplification of target DNA sequences in the form of single or double stranded DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed along with methods for detecting such amplified target sequences wherein some or all of the deoxyribonucleotides are replaced by deoxyribonucleotide analogues that reduce the Tm of the amplified product. The product of this amplification is used for DNA sequencing and other analyses that involve hybridization. Kits containing components for use in the invention is also described. Also described are further uses of this amplified DNA in sequencing, single base substitution detection, modifying the restriction enzyme fragmentation patterns and other molecular biology applications.

The invention discloses an HCBP 6 (Hepatitis C Virus Core-Binding Protein 6) gene knockout cell line and a construction method thereof. The construction method for the HCBP 6 gene knockout cell line is characterized in that a CRISPER/Cas9 (CRISPR associated protein 9) technology is adopted to construct the HCBP 6 gene knockout cell line, a fragment which conforms to a 5'-N[X]-NGG-3' or 5'-CNN-N[X]-3' sequence arrangement rule in the coding sequence of HCPB6 protein is taken as a target sequence, wherein N presents any one of A, G, C and T, X is greater than or equal to 14 or less than or equalto 30, in addition, X is an integer, and N[X] presents X continuous deoxyribonucleotide. The construction method lays a foundation for HCV (hepatitis C virus) infection mechanism research. The HCBP 6gene knockout constructed by the invention forms frameshift mutation on a genome level, the gene can be transferred to the next generation along with the division and the proliferation of cells, anda stable HCBP 6 gene knockout cell line is formed.

Oligonucleotides directed against the survivin gene are provided for modulating the expression of survivin. The compositions comprise oligonucleotides, particularly antisense oligonucleotides, targeted to nucleic acids encoding the survivin. Methods of using these compounds for modulation of survivin expression and for the treatment of diseases associated with either overexpression of survivin, expression of mutated survivin or both are provided. Examples of diseases are cancer such as lung, breast, colon, prostate, pancreas, lung, liver, thyroid, kidney, brain, testes, stomach, intestine, bowel, spinal cord, sinuses, bladder, urinary tract or ovaries cancers. The oligonucleotides may be composed of deoxyribonucleosides or a nucleic acid analogue such as for example locked nucleic acid or a combination thereof.

The invention discloses a method for identifying or assisting in identifying shapes of potatoes and a special primer pair for the method. The method comprises the following steps: detecting whether the 805th deoxyribonucleotide of a target gene on a homologue of a potato to be tested is C or not, the 896th deoxyribonucleotide is T or not and the 1146th deoxyribonucleotide is A or not. The primer pair consists of DNA molecules shown in the sequence 1 of a sequence chart and DNA molecules shown in the sequence 2 of the sequence chart. Experiments show that when the primer pair is used for detecting the potato material, the fit goodness between the detection result and the phenotype identification result is 80% or above, and the fit goodness between the detection result and the round potato shape is 90% or above. The selection accuracy is high, the stability is good, the offspring can be selected in the seedling stage, influences from the plant growth stage and environmental conditions are prevented, the shape breeding process of potatoes can be effectively accelerated, and the breeding cost is lowered.