Method for assisting judgment of Wuzhishan minipig inbred line
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for assisting identification and miniature pigs, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc.
Inactive Publication Date: 2009-12-23
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
View PDF1 Cites 7 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, due to various reasons, it has not been realized. The key is that West Germany does not easily send resources to China through cooperative research.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0021] Embodiment 1, the discovery of Pnas-4 gene C1400T polymorphism
[0022] The pigs used in the experiment were as follows: 13 Landrace pigs, 19 Large White pigs, 45 Tongcheng pigs, 42 Guizhou miniature pigs, 49 Guangxi Bama miniature pigs and 42 Wuzhishan miniature pig inbred lines.
[0023] 1. PCR amplification
[0024] A pair of primers were designed according to the second intron and the 3'UTR sequence of the Pnas-4 gene (GenBank Accession Number DQ406743) (sequence 1 of the sequence listing) as follows:
[0025] Upstream primer (sequence 2 of the sequence listing): 5'-CTAGAACCACTCAAACCAAGCAGC-3';
[0026] Downstream primer (sequence 3 of the sequence listing): 5'-ATCAGGCAGGTAAAAGGATAACGG-3'.
[0027] Utilizing the above primer pairs, PCR amplification was carried out using pig genomic DNA as a template.
[0047] Embodiment 2, the application of Pnas-4 gene C1400T polymorphism
[0048] The 75 ear skin tissue samples were all collected from the Wuzhishan miniature pig inbred line of the Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, of which F 13 Generation 15 heads, F 14 Generation 13 heads, F 15 Generation 11 head, F 16 Generation 14 heads, F 17 Generation 14 heads, F 18 Generation 8, all samples were treated with 75% alcohol and stored in -20°C refrigerator for DNA extraction.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for assisting the judgment of a Wuzhishan minipig inbred line. The method for assisting the judgment of the Wuzhishan minipig inbred line and / or a non-Wuzhishan-mountain minipig inbred line, which is provided by the invention, detects that the 1400th position of deoxyribonucleotide from the 5' end of GenBankAccession Number DQ406743 of a pig to be detected is C or T, and determines that the genotype of the pig to be detected is CC, CT or TT; the pig to be detected with the CC genotype is an alternate Wuzhishan minipig inbred line; and a pig with the CT genotype and a pig to be detected with the TT genotype are non-Wuzhishan-minipig inbred lines. The method can be applied to the seed breeding of the Wuzhishan minipig inbred line. The method can firstly carry out preliminary screening on all the pigs in a swinery to be detected, eliminates the non-Wuzhishan-minipig inbred line, finds out the alternate Wuzhishan minipig inbred line and combines with other methods to carry out further validation. The invention can also be used for detecting whether the Wuzhishan minipig inbred line purchased in the market is counterfeit or not.
Description
technical field [0001] The invention relates to a method for assisting identification of inbred lines of Wuzhishan miniature pigs. Background technique [0002] In the early days of my country's reform and opening up, when researchers were studying in West Germany from 1983 to 1984, they had the honor to visit the Gottengen Minieture Pig Breeding Center in West Germany and learned that miniature pigs have been widely used in animal models of human diseases in Western countries. At the same time, it is an excellent research object for pig embryoengineering. Göttingen minipigs have been sold to many countries and regions, so the idea of bringing them back to China for development and application came into being. Professor Schmidt, one of the miniature pig breeding experts in Göttingen, West Germany, is interested in the Chinese Meishan pig. He suggested that the West German Ministry of Agriculturelist it as a special support for bilateral cooperative research, and the resea...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
IPC IPC(8): C12Q1/68C12N15/11A01K67/027
Inventor 冯书堂杨述林李奎牟玉莲
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Login to view more 
Login to view more