11 results about "Pluronic F68" patented technology

The invention provides a composite quality control product of an AIDS antibody, a syphilis antibody and a hepatitis C antibody as well as a preparation method and application of the composite quality control product. The composite quality control product comprises an AIDS (Acquired Immune Deficiency Syndrome) antibody, a syphilis antibody, a hepatitis C antibody and the following components: a matrix solution, 1.5 to 3 g / L of Pluronic F68, 0.05 to 0.15% v / v of ProClin 300, 0.1 to 1 g / L of sodium surfactin, 1 to 10 g / L of bovine serum albumin, 1 g / L of alanine, 2 to 5 g / L of trehalose, 0.5 to 1.0 g / L of EDTA (Ethylene Diamine Tetraacetic Acid) and 2 to 5 mmol / L of benzamidine. The matrix liquid comprises a buffer solution and 20-30% v / v human serum. According to the invention, the aggregation and inactivation risk of the antibody in the freezing and thawing process is effectively reduced, the antibody can be repeatedly frozen and thawed for more than three times, the degradation of the antibody at high temperature can be effectively prevented, and the reliability of the quality control product in the transportation and clinical use process is ensured.

The invention belongs to the technical field of hybridoma cell culture, and particularly relates to a serum-free culture medium of hybridoma cells, in-vitro suspension culture and application. According to the serum-free culture medium for the hybridoma cells, the polyethylene glycol and the Pluronic F68 are added at the same time and can cooperate with each other, cell membranes of the hybridoma cells are protected, growth and proliferation of the hybridoma cells are promoted, and antibody protein is secreted; the content of the added standby carbon source sodium pyruvate and genetic material raw materials purine and pyrimidine is high, and the types and the content of the added vitamins and amino acids are high, so that the requirements in the aspects of nutrient substances, carbon sources, genetic material raw materials and the like for rapid growth of the hybridoma cells can be met; therefore, the technical problem that a new serum-free hybridoma cell culture medium is lacked in the prior art is solved.

Provided herein are pharmaceutical compositions comprising an adeno-associated virus (AAV). In some aspects, a pharmaceutical composition comprises from about 5 mM to about 20 mM of a Tris buffer, from about 100 mM to about 250 mM of sodium chloride, from about 0.001% (w / v) to about 0.01% (w / v) of Pluronic F68, and has a pH of from 7.0 to 9.0. Also provided are methods of making a pharmaceutical composition comprising the AAV, methods of treating a VEGF-associated condition in a subject, and methods of storing the AAV composition.

The present application relates to a curcumin nanocrystal composition, the composition contains 1-18% (m / v) of curcumin nanocrystal, 0.1-10% (m / v) of stabilizer, 0.1-10% (m / v) of surfactant and 0-10% (m / v) of suspending agent in mass volume percentage, wherein the particle size of the curcumin nanocrystal is less than or equal to 100 nm, the stabilizer is selected from any one or combination of hydroxypropyl methyl cellulose (HPMC), polyvinylpyrrolidone PVPK12PF, polyvinylpyrrolidone PVPK15, polyvinylpyrrolidone PVPK17PF, lecithin, polyethylene glycol, poloxamer Pluronics F68, poloxamer Pluronics F108, the surfactant is selected from any one or combination of sodium cholate, sodium deoxycholate, sodium docusate, sodium dodecyl sulfate (SDS), sodium dodecyl sulfonate (SLS), sodium alginate, and the suspending agent is selected from any one or combination of sucrose, dextran, glycerol, povidone, hydroxypropyl cellulose, methyl cellulose, hypromellose, sodium carboxymethyl cellulose, carboxymethyl cellulose, polyvinyl alcohol, gum arabic, dextrin. The composition of the present application has the advantages of fast onset, good stability, high bioavailability, safety and effectiveness.

Pharmaceutical compositions comprising adeno-associated virus (AAV) are provided herein. In some aspects, the pharmaceutical compositions comprise about 5 mM to about 20 mM Tris buffer, about 100 mM to about 250 mM sodium chloride, about 0.001 % (w / v) to about 0.01 % (w / v) Pluronic F68, and has a pH between 7.0 and 9.0. Methods of preparing a pharmaceutical composition comprising AAV, methods of treating a VEGF-associated disorder in a subject, and methods of storing AAV compositions are also provided.

The application provides a complex quality control product of HIV antibody, syphilis antibody and hepatitis C antibody and a preparation method and application thereof. The complex quality control product comprises HIV antibody, syphilis antibody and hepatitis C antibody and the following components: a matrix liquid, 1.5-3 g / L Pluronic F68, 0.05-0.15 % v / v ProClin 300, 0.1-1 g / L sodium mycobacterial lipopeptide, 1-10 g / L bovine serum albumin, 1 g / L alanine, 2-5 g / L trehalose, 0.5-1.0 g / L EDTA and 2-5 mmol / L benzamidine; the matrix liquid comprises a buffer and 20-30 % v / v human serum. The application effectively reduces the aggregation and inactivation risk of the antibodies in the freeze-thaw process, can be repeatedly freeze-thawed more than three times, can effectively prevent the degradation of the antibodies under high temperature, and ensures the reliability of the quality control product in the transportation and clinical use process.

The application discloses a pH-induced photothermal enhancement type cellulose hydrogel and a preparation method and application thereof. The pH-induced photothermal enhancement type cellulose hydrogel is prepared by the following steps: preparing cellulose nanocrystals with pH / near-infrared light responsiveness by chemically grafting intrinsic state polyaniline on the surface of carboxylated cellulose nanocrystals, preparing pH-responsive drug-loaded cellulose nanocrystals by chemically modifying anti-inflammatory drugs on the surface of dialdehyde cellulose nanocrystals, and assembling the drug-loaded micelles formed by coating a carbon monoxide donor, manganese pentacarbonyl bromide, with a temperature-sensitive reagent, pluronic F68, into the pH-induced photothermal enhancement type cellulose hydrogel. The pH-induced photothermal enhancement type cellulose hydrogel has the pH-induced photothermal enhancement performance, can generate carbon monoxide under hydrogen peroxide stimulation, can controllably release anti-inflammatory drugs under pH stimulation, and can respond to sol-gel transition above 33 DEG C.

The present invention provides a drug delivery system targeting the blood-brain barrier, and its preparation method and application. The drug delivery system includes a nanocarrier and a drug, wherein the nanocarrier includes a polyamidoamine modified with poloxamer 188. The present invention creatively obtains a nanocarrier by simply modifying the PAMAM-NH2 surface with Pluronic F68, thereby simultaneously endowing the carrier with multiple functions: 1) long circulation; 2) high safety and biocompatibility; 3) inhibition of the passive physical barrier of the BBB and weakening of the active barrier of the BBB; 4) significant increase in cellular drug uptake and good drug sustained-release effect. The nanocarrier has a simple design, low cost, and is more suitable for clinical application, providing new ideas for the design of intelligent nano-drug delivery systems for anti-glioma.

The invention provides a preparation method of an enrofloxacin suspension with high stability and release rate, and belongs to the technical field of medicine. The enrofloxacin suspension is prepared from sodium phenylpropionate, disodium ethylene diamine tetraacetate, Pluronic F68, sodium lactate, carboxymethyl cellulose, glycerol, propylene glycol, enrofloxacin and water. Through reasonable proportioning and synergistic effect of all the components, the suspension has excellent stability in a high-temperature environment, degradation of drugs can be remarkably reduced, and the stability of the drugs in high-temperature storage and transportation is improved. Moreover, the synergistic effect of different components not only improves the stability, but also accelerates the release rate of the medicine. Therefore, the enrofloxacin suspension disclosed by the invention is suitable for use and storage requirements of medicines in tropical, subtropical and other high-temperature environments.

The invention relates to the field of petrochemical engineering, in particular to a ZSM-5 molecular sieve with high dispersity and low silica-alumina ratio as well as a preparation method and application of the ZSM-5 molecular sieve. The preparation method provided by the invention comprises the following steps: mixing water, a silicon source, a seed crystal and a mineralizing agent to obtain a mixed solution 1; mixing water, an aluminum source and an alkaline substance to obtain a mixed solution 2; mixing the mixed solution 1 with the mixed solution 2 to obtain a mixed solution 3; mixing the mixed solution 3 with an additive and a template agent to obtain a mixed solution 4; stirring and aging the mixed solution 4, and then crystallizing to obtain a crystallized material; carrying out ammonia exchange on the crystallized material by using an NH4Cl solution, and then calcining to obtain a ZSM-5 molecular sieve; wherein the additive is selected from at least one of poloxamer 188, poloxamer 338, poloxamer 407, Pluronic F127 and Pluronic F68, and the additive is selected from at least one of poloxamer 188, poloxamer 338, poloxamer 407, Pluronic F127 and Pluronic F68. According to the preparation method, the molecular sieve with high dispersity can be obtained.

The invention discloses a preparation method of dope-dyed polyester staple fibers for vortex spinning, which comprises the following steps: mixing polyethylene glycol terephthalate (PET) slices and a nanoscale inorganic pigment according to a mass ratio of (95-98): (2-5), drying in a vacuum drying oven at 120-135 DEG C for 4-6 hours, enabling the moisture content of the slices to be less than or equal to 50ppm, and preparing the dope-dyed polyester staple fibers for vortex spinning by using the surface-modified nano inorganic pigment, thereby obtaining the dope-dyed polyester staple fibers for vortex spinning. The particle size of the pigment is smaller than or equal to 200 nm, the Zeta potential is larger than 30 mV, and the color fastness is improved to the 4-5 level through the cooperation of the SiO coated TiO / FeO compound and the Pluronic F68 block dispersing agent, the particle size of the pigment is smaller than or equal to 200 nm, and the Zeta potential is larger than 30 mV.